PMID- 31330263
OWN - NLM
STAT- MEDLINE
DCOM- 20200323
LR  - 20200323
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 136
DP  - 2019 Nov
TI  - Characterization of the antioxidant and anti-inflammatory properties of a 
      polysaccharide-based bioflocculant from Bacillus subtilis F9.
PG  - 103642
LID - S0882-4010(19)31089-7 [pii]
LID - 10.1016/j.micpath.2019.103642 [doi]
AB  - Microbial flocculants are versatile class of novel biomacromolecules with 
      numerous potential industrial applications. This study sought to investigate the 
      antimicrobial, antioxidant, and anti-inflammatory potential of a 
      polysaccharide-based bioflocculant (PBB) extracted from Bacillus subtilis F9. To 
      achieve this, the antioxidant activity of different PBB concentrations(100 mug/mL 
      ̶ 1000 mug/mL) was first examined in vitro using 2,2-diphenyl-1-picrylhydrazyl 
      (DPPH), hydroxyl radicals, and superoxide radical scavenging assays. Further, the 
      anti-inflammatory activity of PBB against lipopolysaccharide (LPS; 
      1 mug/mL)-induced inflammatory mediators released from headkidney (HK)-derived 
      macrophages of Labeo rohita was investigated. Our results revealed that the 
      capacities of 800 mug/mL of PBB to scavenge DPPH, hydroxyl radicals, and 
      superoxide radicals were 81.46 +/- 1.37%, 66.34 +/- 2.63%, and 78.03 +/- 2.46%, 
      respectively, which were slightly higher that observed following treatment with 
      400 mug/mL of the positive control (ascorbic acid). Further, the radical 
      scavenging capacity of PBB was found to steadily increase with increasing 
      concentrations of PBB. Pre-treatment with PBB also inhibited nitric oxide 
      production in a dose-dependent manner. We next examined the effect of PBB on 
      proinflammatory cytokines (TNF-alpha, and IL-1beta) and anti-inflammatory cytokines 
      (IL-10, TGF-beta) via qRT-PCR and ELISA. We found that PBB markedly inhibited the 
      LPS-induced mRNA and protein expression levels of TNF-alpha and IL-1beta, while it 
      significantly increased those of IL-10 and TGF-beta. Further, PBB exhibited an 
      antibacterial activity against multiple food-borne pathogens with minimal 
      inhibitory concentration values in the range of 3 ̶ 11 mg/mL. Importantly, PBB 
      exhibited negligible cytotoxic effects against HK macrophages. Taken together 
      these results suggest that PBB may serve as a natural antioxidant for application 
      in functional therapies and may also be exploited for its anti-inflammatory 
      potential.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Giri, Sib Sankar
AU  - Giri SS
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, 08826, South 
      Korea. Electronic address: giribiotek@gmail.com.
FAU - Ryu, EunChae
AU  - Ryu E
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, 08826, South 
      Korea. Electronic address: luckyvet@naver.com.
FAU - Park, Se Chang
AU  - Park SC
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, 08826, South 
      Korea. Electronic address: parksec@snu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20190719
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Cytokines)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 11062-77-4 (Superoxides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3352-57-6 (Hydroxyl Radical)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*isolation & purification/*pharmacology
MH  - Antioxidants/*isolation & purification/*pharmacology
MH  - Bacillus subtilis/*chemistry
MH  - Cells, Cultured
MH  - Cyprinidae
MH  - Cytokines/metabolism
MH  - Hydroxyl Radical/metabolism
MH  - Macrophages/drug effects/metabolism
MH  - Nitric Oxide/metabolism
MH  - Polysaccharides, Bacterial/*isolation & purification/*pharmacology
MH  - Superoxides/metabolism
OTO - NOTNLM
OT  - Antibacterial
OT  - Antioxidant
OT  - Polysaccharide-based bioflocculant
EDAT- 2019/07/23 06:00
MHDA- 2020/03/24 06:00
CRDT- 2019/07/23 06:00
PHST- 2019/06/16 00:00 [received]
PHST- 2019/07/04 00:00 [revised]
PHST- 2019/07/19 00:00 [accepted]
PHST- 2019/07/23 06:00 [pubmed]
PHST- 2020/03/24 06:00 [medline]
PHST- 2019/07/23 06:00 [entrez]
AID - S0882-4010(19)31089-7 [pii]
AID - 10.1016/j.micpath.2019.103642 [doi]
PST - ppublish
SO  - Microb Pathog. 2019 Nov;136:103642. doi: 10.1016/j.micpath.2019.103642. Epub 2019 
      Jul 19.