PMID- 31332273
OWN - NLM
STAT- MEDLINE
DCOM- 20201023
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Jul 22
TI  - Identification of novel breakpoints for locus- and region-specific translocations 
      in 293 cells by molecular cytogenetics before and after irradiation.
PG  - 10554
LID - 10.1038/s41598-019-47002-0 [doi]
LID - 10554
AB  - The human kidney embryonic 293 cell line (293 cells) is extensively used in 
      biomedical and pharmaceutical research. These cells exhibit a number of numerical 
      and structural chromosomal anomalies. However, the breakpoints responsible for 
      these structural chromosomal rearrangements have not been comprehensively 
      characterized. In addition, it is not known whether chromosomes with structural 
      rearrangement are more sensitive to external toxic agents, such as ionizing 
      radiation. We used G-banding, spectral karyotyping (SKY), and locus- and 
      region-specific fluorescence in situ hybridization (FISH) probes designed in our 
      lab or obtained from commercial vendor to address this gap. Our G-banding 
      analysis revealed that the chromosome number varies from 66 to 71, with multiple 
      rearrangements and partial additions and deletions. SKY analysis confirmed 3 
      consistent rearrangements, two simple and one complex in nature. Multicolor FISH 
      analysis identified an array of breakpoints responsible for locus- and 
      region-specific translocations. Finally, SKY analysis revealed that 
      radio-sensitivity of structurally rearranged chromosomes is dependent on 
      radiation dose. These findings will advance our knowledge in 293 cell biology and 
      will enrich the understanding of radiation biology studies.
FAU - Binz, Regina L
AU  - Binz RL
AUID- ORCID: 0000-0002-4689-3073
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
FAU - Tian, Erming
AU  - Tian E
AD  - Myeloma Center, Winthrop P. Rockefeller Cancer Institute, University of Arkansas 
      for Medical Sciences, Little Rock, AR, USA.
FAU - Sadhukhan, Ratan
AU  - Sadhukhan R
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
FAU - Zhou, Daohong
AU  - Zhou D
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
AD  - Department of Pharmacodynamics, College of Pharmacy, Gainesville, FL, USA.
FAU - Hauer-Jensen, Martin
AU  - Hauer-Jensen M
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
FAU - Pathak, Rupak
AU  - Pathak R
AUID- ORCID: 0000-0002-6239-301X
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR, USA. 
      rpathak@uams.edu.
LA  - eng
GR  - P20 GM109005/GM/NIGMS NIH HHS/United States
GR  - R01 CA211963/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190722
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosome Breakpoints/*radiation effects
MH  - Chromosome Painting
MH  - Cytogenetics
MH  - Gene Rearrangement/radiation effects
MH  - HEK293 Cells
MH  - Humans
MH  - Radiation Tolerance/genetics
MH  - Spectral Karyotyping
MH  - Translocation, Genetic/*radiation effects
PMC - PMC6646394
COIS- The authors declare no competing interests.
EDAT- 2019/07/25 06:00
MHDA- 2020/10/24 06:00
PMCR- 2019/07/22
CRDT- 2019/07/24 06:00
PHST- 2019/03/01 00:00 [received]
PHST- 2019/06/24 00:00 [accepted]
PHST- 2019/07/24 06:00 [entrez]
PHST- 2019/07/25 06:00 [pubmed]
PHST- 2020/10/24 06:00 [medline]
PHST- 2019/07/22 00:00 [pmc-release]
AID - 10.1038/s41598-019-47002-0 [pii]
AID - 47002 [pii]
AID - 10.1038/s41598-019-47002-0 [doi]
PST - epublish
SO  - Sci Rep. 2019 Jul 22;9(1):10554. doi: 10.1038/s41598-019-47002-0.