PMID- 31332976
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 11
IP  - 5
DP  - 2019 Sep
TI  - Survey of IgE Reactivity to Nonbiting Midges in Korea and Identification of 
      IgE-Binding Protein.
PG  - 644-654
LID - 10.4168/aair.2019.11.5.644 [doi]
AB  - PURPOSE: Chironomids (nonbiting midges) are widely and abundantly distributed 
      near ponds, rivers, and artificially dammed pools used for irrigation. 
      Chironomids contain allergens and cause airway allergy in humans. In this study, 
      we aimed to examine the allergic potential of chironomids in inhabitants living 
      near artificially dammed pools. METHODS: We examined immunoglobulin E (IgE) 
      reactivity to chironomid extracts in the sera of residents living around 
      installed dams and assessed the correlations of IgE responses between chironomids 
      (Chironomus flaviplumus, Chironomus kiiensis, Cricotopus bicinctus) and house 
      dust mites (Dermatophagoides farinae). In addition, we identified potential IgE 
      binding proteins specific for adult C. bicinctus, a popular species in Korea. 
      Specific IgE antibodies in sera collected from the participants against the 
      extracts were tested using enzyme-linked immunosorbent assay (ELISA). RESULTS: 
      The average IgE-positive rates were 10.4%, 8.1%, and 8.2% in C. bicinctus, C. 
      flaviplumus, and C. kiiensis, respectively. The IgE-positive rate and IgE titer 
      of C. bicinctus antigen were higher in residents living around installed dams 
      than in those who lived other places (P = 0.013). Western blotting using sera 
      having high IgE titers to C. bicinctus in ELISA showed the presence of a protein 
      of approximately 42 kDa that was homologous to the actin protein isoform in C. 
      bicinctus extracts as demonstrated using mass spectrometry. CONCLUSIONS: Our 
      results showed that people living near installed dams were more sensitized to C. 
      bicinctus and that the 42 kDa IgE-binding protein could be useful for further 
      studies on chironomid allergic disease and clinical applications.
CI  - Copyright (c) 2019 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease.
FAU - Yi, Myung Hee
AU  - Yi MH
AUID- ORCID: 0000-0001-9537-5726
AD  - Department of Environmental Medical Biology, Arthropods of Medical Importance 
      Resource Bank, Institute of Tropical Medicine, Yonsei University College of 
      Medicine, Seoul, Korea.
FAU - Kim, Ju Yeong
AU  - Kim JY
AUID- ORCID: 0000-0003-2456-6298
AD  - Department of Environmental Medical Biology, Arthropods of Medical Importance 
      Resource Bank, Institute of Tropical Medicine, Yonsei University College of 
      Medicine, Seoul, Korea.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AUID- ORCID: 0000-0001-9887-1426
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul, Korea.
FAU - Ree, Han Il
AU  - Ree HI
AD  - Department of Environmental Medical Biology, Arthropods of Medical Importance 
      Resource Bank, Institute of Tropical Medicine, Yonsei University College of 
      Medicine, Seoul, Korea.
FAU - Yong, Tai Soon
AU  - Yong TS
AD  - Department of Environmental Medical Biology, Arthropods of Medical Importance 
      Resource Bank, Institute of Tropical Medicine, Yonsei University College of 
      Medicine, Seoul, Korea. tsyong212@yuhs.ac.
LA  - eng
GR  - NRF-2012M3A9B8021806/NRF/National Research Foundation of Korea/Korea
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC6658401
OTO - NOTNLM
OT  - IgE binding protein
OT  - Nonbiting midges
OT  - allergy
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2019/07/25 06:00
MHDA- 2019/07/25 06:01
PMCR- 2019/09/01
CRDT- 2019/07/24 06:00
PHST- 2018/12/30 00:00 [received]
PHST- 2019/04/06 00:00 [revised]
PHST- 2019/05/01 00:00 [accepted]
PHST- 2019/07/24 06:00 [entrez]
PHST- 2019/07/25 06:00 [pubmed]
PHST- 2019/07/25 06:01 [medline]
PHST- 2019/09/01 00:00 [pmc-release]
AID - 11.644 [pii]
AID - 10.4168/aair.2019.11.5.644 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2019 Sep;11(5):644-654. doi: 
      10.4168/aair.2019.11.5.644.