PMID- 31336886
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231104
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 11
IP  - 7
DP  - 2019 Jul 12
TI  - Genetic Aberration Analysis in Thai Colorectal Adenoma and Early-Stage 
      Adenocarcinoma Patients by Whole-Exome Sequencing.
LID - 10.3390/cancers11070977 [doi]
LID - 977
AB  - Colorectal adenomas are precursor lesions of colorectal adenocarcinoma. The 
      transition from adenoma to carcinoma in patients with colorectal cancer (CRC) has 
      been associated with an accumulation of genetic aberrations. However, criteria 
      that can screen adenoma progression to adenocarcinoma are still lacking. This 
      present study is the first attempt to identify genetic aberrations, such as the 
      somatic mutations, copy number variations (CNVs), and high-frequency mutated 
      genes, found in Thai patients. In this study, we identified the genomic 
      abnormality of two sample groups. In the first group, five cases matched 
      normal-colorectal adenoma-colorectal adenocarcinoma. In the second group, six 
      cases matched normal-colorectal adenomas. For both groups, whole-exome sequencing 
      was performed. We compared the genetic aberration of the two sample groups. In 
      both normal tissues compared with colorectal adenoma and colorectal 
      adenocarcinoma analyses, somatic mutations were observed in the tumor suppressor 
      gene APC (Adenomatous polyposis coli) in eight out of ten patients. In the group 
      of normal tissue comparison with colorectal adenoma tissue, somatic mutations 
      were also detected in Catenin Beta 1 (CTNNB1), Family With Sequence Similarity 
      123B (FAM123B), F-Box And WD Repeat Domain Containing 7 (FBXW7), Sex-Determining 
      Region Y-Box 9 (SOX9), Low-Density Lipoprotein Receptor-Related Protein 5 (LRP5), 
      Frizzled Class Receptor 10 (FZD10), and AT-Rich Interaction Domain 1A (ARID1A) 
      genes, which are involved in the Wingless-related integration site (Wnt) 
      signaling pathway. In the normal tissue comparison with colorectal adenocarcinoma 
      tissue, Kirsten retrovirus-associated DNA sequences (KRAS), Tumor Protein 53 
      (TP53), and Ataxia-Telangiectasia Mutated (ATM) genes are found in the receptor 
      tyrosine kinase-RAS (RTK-RAS) signaling pathway and p53 signaling pathway, 
      respectively. These results suggest that APC and TP53 may act as a potential 
      screening marker for colorectal adenoma and early-stage CRC. This preliminary 
      study may help identify patients with adenoma and early-stage CRC and may aid in 
      establishing prevention and surveillance strategies to reduce the incidence of 
      CRC.
FAU - Intarajak, Thoranin
AU  - Intarajak T
AD  - Bioinformatics and Systems Biology Program, School of Bioresources and Technology 
      and School of Information Technology, King Mongkut's University of Technology 
      Thonburi, Bangkok 10150, Thailand.
AD  - Bioinformatics Unit for Genomic Analysis, Division of Research and International 
      Relations, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal 
      Academy, Bangkok 10210, Thailand.
AD  - Systems Biology and Bioinformatics Research Group, Pilot Plant Development and 
      Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 
      10150, Thailand.
FAU - Udomchaiprasertkul, Wandee
AU  - Udomchaiprasertkul W
AD  - Molecular Biology and Genomic Laboratory, Division of Research and International 
      Relations, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal 
      Academy, Bangkok 10210, Thailand.
FAU - Bunyoo, Chakrit
AU  - Bunyoo C
AD  - Bioinformatics Unit for Genomic Analysis, Division of Research and International 
      Relations, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal 
      Academy, Bangkok 10210, Thailand.
FAU - Yimnoon, Jutamas
AU  - Yimnoon J
AD  - Cytogenetics Unit, Central Research Laboratory, Division of Research and 
      International Relations, HRH Princess Chulabhorn College of Medical Science, 
      Chulabhorn Royal Academy, Bangkok 10210, Thailand.
FAU - Soonklang, Kamonwan
AU  - Soonklang K
AD  - Data Management Unit, HRH Princess Chulabhorn College of Medical Science, 
      Chulabhorn Royal Academy, Bangkok 10210, Thailand.
FAU - Wiriyaukaradecha, Kriangpol
AU  - Wiriyaukaradecha K
AD  - Molecular Biology and Genomic Laboratory, Division of Research and International 
      Relations, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal 
      Academy, Bangkok 10210, Thailand.
FAU - Lamlertthon, Wisut
AU  - Lamlertthon W
AD  - Faculty of Medicine and Public Health, HRH Princess Chulabhorn College of Medical 
      Science, Chulabhorn Royal Academy, Bangkok 10210, Thailand.
FAU - Sricharunrat, Thaniya
AU  - Sricharunrat T
AD  - Pathology Laboratory Unit, Chulabhorn Hospital, HRH Princess Chulabhorn College 
      of Medical Science, Chulabhorn Royal Academy, Bangkok 10210, Thailand.
FAU - Chaiwiriyawong, Worawit
AU  - Chaiwiriyawong W
AD  - Department of Medical Oncology, Chulabhorn Hospital, HRH Princess Chulabhorn 
      College of Medical Science, Chulabhorn Royal Academy, Bangkok 10210, Thailand.
FAU - Siriphongpreeda, Bunchorn
AU  - Siriphongpreeda B
AD  - Faculty of Medicine and Public Health, HRH Princess Chulabhorn College of Medical 
      Science, Chulabhorn Royal Academy, Bangkok 10210, Thailand.
FAU - Sutheeworapong, Sawannee
AU  - Sutheeworapong S
AD  - Bioinformatics and Systems Biology Program, School of Bioresources and Technology 
      and School of Information Technology, King Mongkut's University of Technology 
      Thonburi, Bangkok 10150, Thailand.
AD  - Systems Biology and Bioinformatics Research Group, Pilot Plant Development and 
      Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 
      10150, Thailand.
FAU - Kusonmano, Kanthida
AU  - Kusonmano K
AD  - Bioinformatics and Systems Biology Program, School of Bioresources and Technology 
      and School of Information Technology, King Mongkut's University of Technology 
      Thonburi, Bangkok 10150, Thailand.
AD  - Systems Biology and Bioinformatics Research Group, Pilot Plant Development and 
      Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 
      10150, Thailand.
AD  - School of Bioresources and Technology, King Mongkut's University of Technology 
      Thonburi, Bangkok 10150, Thailand.
FAU - Kittichotirat, Weerayuth
AU  - Kittichotirat W
AUID- ORCID: 0000-0001-8397-633X
AD  - Bioinformatics and Systems Biology Program, School of Bioresources and Technology 
      and School of Information Technology, King Mongkut's University of Technology 
      Thonburi, Bangkok 10150, Thailand.
AD  - Systems Biology and Bioinformatics Research Group, Pilot Plant Development and 
      Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 
      10150, Thailand.
FAU - Thammarongtham, Chinae
AU  - Thammarongtham C
AD  - Biochemical Engineering and Systems Biology research group, National Center for 
      Genetic Engineering and Biotechnology (BIOTEC) at King Mongkut's University of 
      Technology Thonburi, Bangkhuntien, Bangkok 10150, Thailand.
FAU - Jenjaroenpun, Piroon
AU  - Jenjaroenpun P
AUID- ORCID: 0000-0002-1555-401X
AD  - Department of Biomedical Informatics, College of Medicine, University of Arkansas 
      for Medical Sciences, Little Rock, AR 72205, USA.
FAU - Wongsurawat, Thidathip
AU  - Wongsurawat T
AUID- ORCID: 0000-0002-3659-2074
AD  - Department of Biomedical Informatics, College of Medicine, University of Arkansas 
      for Medical Sciences, Little Rock, AR 72205, USA.
FAU - Nookaew, Intawat
AU  - Nookaew I
AD  - Department of Biomedical Informatics, College of Medicine, University of Arkansas 
      for Medical Sciences, Little Rock, AR 72205, USA.
AD  - Department of Biology and Biological Engineering, Chalmers University of 
      Technology, SE-412 96 Gothenburg, Sweden.
AD  - Department of Physiology and Biophysics, College of Medicine, The University of 
      Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
FAU - Auewarakul, Chirayu
AU  - Auewarakul C
AD  - Faculty of Medicine and Public Health, HRH Princess Chulabhorn College of Medical 
      Science, Chulabhorn Royal Academy, Bangkok 10210, Thailand. 
      chirayu.aue@cra.ac.th.
FAU - Cheevadhanarak, Supapon
AU  - Cheevadhanarak S
AD  - Systems Biology and Bioinformatics Research Group, Pilot Plant Development and 
      Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 
      10150, Thailand. supapon.che@mail.kmutt.ac.th.
AD  - School of Bioresources and Technology, King Mongkut's University of Technology 
      Thonburi, Bangkok 10150, Thailand. supapon.che@mail.kmutt.ac.th.
LA  - eng
GR  - P20 GM125503/GM/NIGMS NIH HHS/United States
GR  - 002.07.09/HRH princess Chulabhorn College/
PT  - Journal Article
DEP - 20190712
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC6679221
OTO - NOTNLM
OT  - colorectal adenoma
OT  - early-stage colorectal adenocarcinoma
OT  - screen marker
OT  - somatic mutation
COIS- The authors declare no conflict of interest.
EDAT- 2019/07/25 06:00
MHDA- 2019/07/25 06:01
PMCR- 2019/07/12
CRDT- 2019/07/25 06:00
PHST- 2019/05/01 00:00 [received]
PHST- 2019/05/31 00:00 [revised]
PHST- 2019/06/03 00:00 [accepted]
PHST- 2019/07/25 06:00 [entrez]
PHST- 2019/07/25 06:00 [pubmed]
PHST- 2019/07/25 06:01 [medline]
PHST- 2019/07/12 00:00 [pmc-release]
AID - cancers11070977 [pii]
AID - cancers-11-00977 [pii]
AID - 10.3390/cancers11070977 [doi]
PST - epublish
SO  - Cancers (Basel). 2019 Jul 12;11(7):977. doi: 10.3390/cancers11070977.