PMID- 31338984
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20200731
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 63
IP  - 19
DP  - 2019 Oct
TI  - Quercetin-Induced miR-369-3p Suppresses Chronic Inflammatory Response Targeting 
      C/EBP-beta.
PG  - e1801390
LID - 10.1002/mnfr.201801390 [doi]
AB  - SCOPE: Dendritic cells (DCs) are the most potent antigen-presenting cells that 
      play an important role in the crosstalk between the innate and the adaptive 
      immune response. Quercetin exposure is identified as an effective strategy to 
      suppress the inflammatory response induced by LPS. METHODS AND RESULTS: In this 
      study, using a next-generation sequencing analysis, the effect of quercetin on 
      microRNAs (miRNAs) expression in DCs is examined. A signature of 113 miRNAs that 
      are differentially regulated in LPS-stimulated DCs after quercetin exposure is 
      defined. It is demonstrated that the loss of function of miR-369-3p in 
      LPS-stimulated DCs during quercetin exposure led to an increase of CCAAT/enhancer 
      binding protein beta (C/EBP-beta) mRNA and protein and its downstream targets tumor 
      necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL6). Conversely, it is shown that 
      the ectopic induction of miR-369-3p without quercetin suppresses the inflammatory 
      response of LPS reducing C/EBP-beta, TNF-alpha, and IL6 production. In vivo, oral 
      administration of quercetin in dextran-sulfate-sodium-induced colitis induces 
      miR-369-3p expression. CONCLUSIONS: These findings indicate that 
      quercetin-induced miR-369-3p regulates the inflammatory cascade in chronic 
      inflammatory response and present promising therapeutic implications.
CI  - (c) 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Galleggiante, Vanessa
AU  - Galleggiante V
AD  - National Institute of Gastroenterology "S. de Bellis", Research Hospital, 
      Castellana Grotte, 70013, Bari, Italy.
FAU - De Santis, Stefania
AU  - De Santis S
AD  - Department of Pharmacy, School of Pharmacy, University of Salerno, 84084, 
      Fisciano, Italy.
FAU - Liso, Marina
AU  - Liso M
AD  - National Institute of Gastroenterology "S. de Bellis", Research Hospital, 
      Castellana Grotte, 70013, Bari, Italy.
FAU - Verna, Giulio
AU  - Verna G
AD  - National Institute of Gastroenterology "S. de Bellis", Research Hospital, 
      Castellana Grotte, 70013, Bari, Italy.
FAU - Sommella, Eduardo
AU  - Sommella E
AD  - Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 
      80131, Naples, Italy.
FAU - Mastronardi, Mauro
AU  - Mastronardi M
AD  - National Institute of Gastroenterology "S. de Bellis", Research Hospital, 
      Castellana Grotte, 70013, Bari, Italy.
FAU - Campiglia, Pietro
AU  - Campiglia P
AD  - Department of Pharmacy, School of Pharmacy, University of Salerno, 84084, 
      Fisciano, Italy.
FAU - Chieppa, Marcello
AU  - Chieppa M
AD  - National Institute of Gastroenterology "S. de Bellis", Research Hospital, 
      Castellana Grotte, 70013, Bari, Italy.
FAU - Serino, Grazia
AU  - Serino G
AUID- ORCID: 0000-0002-2971-0802
AD  - National Institute of Gastroenterology "S. de Bellis", Research Hospital, 
      Castellana Grotte, 70013, Bari, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190731
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MIRN369 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - CCAAT-Enhancer-Binding Protein-beta/analysis/*genetics
MH  - Cells, Cultured/chemistry
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Gene Expression/*drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Gene Silencing
MH  - High-Throughput Nucleotide Sequencing
MH  - Inflammation/*genetics
MH  - Interleukin-6/genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/antagonists & inhibitors/genetics/*physiology
MH  - Quercetin/*pharmacology
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/genetics
OTO - NOTNLM
OT  - dendritic cells
OT  - inflammation
OT  - inflammatory bowel disease
OT  - microRNAs
OT  - quercetin
EDAT- 2019/07/25 06:00
MHDA- 2020/06/23 06:00
CRDT- 2019/07/25 06:00
PHST- 2018/12/19 00:00 [received]
PHST- 2019/06/11 00:00 [revised]
PHST- 2019/07/25 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2019/07/25 06:00 [entrez]
AID - 10.1002/mnfr.201801390 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2019 Oct;63(19):e1801390. doi: 10.1002/mnfr.201801390. Epub 
      2019 Jul 31.