PMID- 31342911
OWN - NLM
STAT- MEDLINE
DCOM- 20190801
LR  - 20190801
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 135
IP  - 2
DP  - 2019 Jul 25
TI  - Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a 
      polymicrobial infection in the Pacific oyster Crassostrea gigas.
PG  - 97-106
LID - 10.3354/dao03384 [doi]
AB  - The Pacific oyster Crassostrea gigas is currently being impacted by a 
      polymicrobial disease that involves early viral infection by ostreid 
      herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to 
      death. A widely used method of inducing infection consists of placing specific 
      pathogen-free oysters ('recipients') in cohabitation in the laboratory with 
      diseased oysters that were naturally infected in the field ('donors'). With this 
      method, we evaluated the temporal dynamics of pathogen release in seawater and 
      the cohabitation time necessary for disease transmission and expression. We 
      showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during 
      the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation with 
      donors was enough time to transmit pathogens to recipients and to induce 
      mortality later, reflecting the highly contagious nature of the disease. Finally, 
      mortality of recipients was associated with increasing cohabitation time with 
      donors until reaching a plateau at 20%. This reflects the cumulative effect of 
      exposure to pathogens. The optimal cohabitation time was 5-6 d, the mortality of 
      recipients occurring 1-2 d earlier.
FAU - Petton, Bruno
AU  - Petton B
AD  - Ifremer, LEMAR UMR 6539 (Universite de Bretagne Occidentale, CNRS, IRD, Ifremer), 
      11 presqu'ile du Vivier, 29840 Argenton-en-Landunvez, France.
FAU - de Lorgeril, Julien
AU  - de Lorgeril J
FAU - Mitta, Guillaume
AU  - Mitta G
FAU - Daigle, Gaetan
AU  - Daigle G
FAU - Pernet, Fabrice
AU  - Pernet F
FAU - Alunno-Bruscia, Marianne
AU  - Alunno-Bruscia M
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Crassostrea
MH  - DNA, Viral
MH  - *Herpesviridae
MH  - Seawater
MH  - *Vibrio
OTO - NOTNLM
OT  - Aquaculture
OT  - Bivalve
OT  - Epidemiology
OT  - Health
OT  - OSHV-1
OT  - POMS
OT  - Pacific oyster mortality syndrome
OT  - Polymicrobial disease
EDAT- 2019/07/26 06:00
MHDA- 2019/08/02 06:00
CRDT- 2019/07/26 06:00
PHST- 2019/07/26 06:00 [entrez]
PHST- 2019/07/26 06:00 [pubmed]
PHST- 2019/08/02 06:00 [medline]
AID - 10.3354/dao03384 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2019 Jul 25;135(2):97-106. doi: 10.3354/dao03384.