PMID- 31345961
OWN - NLM
STAT- MEDLINE
DCOM- 20200909
LR  - 20200909
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 39
IP  - 8
DP  - 2019 Aug 30
TI  - Protective effects of delphinidin against H(2)O(2)-induced oxidative injuries in 
      human retinal pigment epithelial cells.
LID - BSR20190689 [pii]
LID - 10.1042/BSR20190689 [doi]
AB  - Age-related macular degeneration (AMD) is now one of the leading causes of 
      blindness in the elderly population and oxidative stress-induced damage to 
      retinal pigment epithelial (RPE) cells occurs as part of the pathogenesis of AMD. 
      In the present study, we evaluated the protective effect of delphinidin 
      (2-(3,4,5-trihydroxyphenyl) chromenylium-3,5,7-triol) against hydrogen peroxide 
      (H(2)O(2))-induced toxicity in human ARPE-19 cells and its molecular mechanism. 
      MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow 
      cytometry demonstrated that pretreatment of ARPE-19 cells with delphinidin (25, 
      50, and 100 mug/ml) significantly increased cell viability and reduced the 
      apoptosis from H(2)O(2) (0.5 mM)-induced oxidative stress in a 
      concentration-dependent manner, which was achieved by the inhibition of Bax, 
      cytochrome c, and caspase-3 protein expression and enhancement of Bcl-2 protein. 
      The same tendency was observed in ARPE-19 cells pre-treated with 15 mM of 
      N-acetylcysteine (NAC) before the addition of H(2)O(2) Furthermore, 
      pre-incubation of ARPE-19 cells with delphinidin markedly inhibited the 
      intracellular reactive oxygen species (ROS) generation and Nox1 protein 
      expression induced by H(2)O(2) Moreover, the decreased antioxidant enzymes 
      activities of superoxide dismutase (SOD), catalase (CAT), and 
      glutathione-peroxidase (GSH-PX) and elevated (MDA) level in H(2)O(2)-treated 
      cells were reversed to the normal standard by the addition of delphinidin, which 
      was regulated by increasing nuclear Nrf2 protein expression in ARPE-19 cells. Our 
      results suggest that delphinidin effectively protects human ARPE-19 cells from 
      H(2)O(2)-induced oxidative damage via anti-apoptotic and antioxidant effects.
CI  - (c) 2019 The Author(s).
FAU - Ni, Timin
AU  - Ni T
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, 
      China.
AD  - Department of Ophthalmology, Wuhan Central Hospital, Wuhan 430014, China.
FAU - Yang, Wanju
AU  - Yang W
AD  - Department of Ophthalmology, Wuhan Central Hospital, Wuhan 430014, China.
FAU - Xing, Yiqiao
AU  - Xing Y
AUID- ORCID: 0000-0002-0980-1598
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, 
      China yiqiaoxingwuhan@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190815
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Anthocyanins)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EM6MD4AEHE (delphinidin)
SB  - IM
MH  - Anthocyanins/*pharmacology
MH  - Cell Line
MH  - Epithelial Cells/*metabolism/pathology
MH  - Humans
MH  - Hydrogen Peroxide/*pharmacology
MH  - Oxidative Stress/*drug effects
MH  - Retinal Pigment Epithelium/*injuries/*metabolism/pathology
PMC - PMC6695502
OTO - NOTNLM
OT  - Age-related macular degeneration
OT  - Antioxidant
OT  - Apoptosis
OT  - Delphinidin
OT  - Human retinal pigment epithelial cells
OT  - Oxidative stress
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2019/07/28 06:00
MHDA- 2020/09/10 06:00
PMCR- 2019/08/15
CRDT- 2019/07/27 06:00
PHST- 2019/03/20 00:00 [received]
PHST- 2019/06/20 00:00 [revised]
PHST- 2019/07/14 00:00 [accepted]
PHST- 2019/07/28 06:00 [pubmed]
PHST- 2020/09/10 06:00 [medline]
PHST- 2019/07/27 06:00 [entrez]
PHST- 2019/08/15 00:00 [pmc-release]
AID - BSR20190689 [pii]
AID - 10.1042/BSR20190689 [doi]
PST - epublish
SO  - Biosci Rep. 2019 Aug 15;39(8):BSR20190689. doi: 10.1042/BSR20190689. Print 2019 
      Aug 30.