PMID- 31346836
OWN - NLM
STAT- MEDLINE
DCOM- 20190809
LR  - 20200225
IS  - 1573-0972 (Electronic)
IS  - 0959-3993 (Linking)
VI  - 35
IP  - 8
DP  - 2019 Jul 26
TI  - A novel unhairing enzyme produced by heterologous expression of keratinase gene 
      (kerT) in Bacillus subtilis.
PG  - 122
LID - 10.1007/s11274-019-2701-2 [doi]
AB  - To promote enzymatic unhairing for leather production, a new unhairing enzyme is 
      developed. The Keratinase (kerT) gene, which is amplified from B. 
      amyloliquefaciens TCCC11319 by PCR, is expressed in B. subtilis WB600. The 
      recombinant KerT reduces the collagenolytic protease content as well as improving 
      the keratinase content effectively. Therefore, the improved keratinase leads to 
      the obviously unhairing effect, whereas the low collagenolytic protease ensures 
      the integrity of collagen fibers in hide. It represents, the leather grain 
      surface isn't destroyed thereby the value of finished leather can be maintained. 
      In addition, by analyzing the properties of KerT, tits activity isn't inhibited 
      with Na(+), K(+) and Ca(2+) which are commonly used in leather production. The 
      freeze-dried fermentation broth can be used directly as unhairing enzyme without 
      addition of traditional sulfide chemicals. By evaluating the properties of 
      unhaired hide, the results show that the collagen degradation ability of this new 
      unhairing enzyme is slightly and it does not cause any adverse effects on the 
      leather quality. Besides, this unhairing enzyme doesn't further degrade collagen 
      in the time range of 8 h to 24 h, thus it is safely and easy-control in actual 
      production. In conclusion, the enzymatic unhairing method with recombinant KerT 
      has the potential to be more sustainable and efficient alternative than current 
      sulphur-lime method, and it does not require the further purification thereby 
      saving the cost.
FAU - Cao, Shan
AU  - Cao S
AUID- ORCID: 0000-0003-3456-4616
AD  - Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin, China. cs1988@foxmail.com.
AD  - School of Light Industry and Engineering, Qilu University of Technology, 
      Shandong, China. cs1988@foxmail.com.
FAU - Li, Dengke
AU  - Li D
AD  - Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin, China.
FAU - Ma, Xiaoxiao
AU  - Ma X
AD  - Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin, China.
FAU - Xin, Qinglong
AU  - Xin Q
AD  - Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin, China.
FAU - Song, Jinzhi
AU  - Song J
AD  - School of Light Industry and Engineering, Qilu University of Technology, 
      Shandong, China.
FAU - Lu, Fuping
AU  - Lu F
AD  - Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin, China.
FAU - Li, Yu
AU  - Li Y
AD  - Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin, China. liyu@tust.edu.cn.
LA  - eng
GR  - 21808170/National Natural Science Foundation of China/
GR  - 2017YFB0308401/Jiangsu Key Laboratory for the Research and Uti1ization of Plant 
      Resources (CN)/
PT  - Journal Article
DEP - 20190726
PL  - Germany
TA  - World J Microbiol Biotechnol
JT  - World journal of microbiology & biotechnology
JID - 9012472
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.- (keratinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacillus subtilis/*enzymology/*genetics
MH  - Bacterial Proteins/genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA Fragmentation
MH  - DNA, Bacterial/genetics/*isolation & purification
MH  - Fermentation
MH  - Gene Expression Regulation, Bacterial
MH  - Genes, Bacterial
MH  - Peptide Hydrolases/*genetics/metabolism
MH  - Recombinant Proteins/genetics/metabolism
OTO - NOTNLM
OT  - Heterologous expression
OT  - KerT gene
OT  - Unhairing enzyme
EDAT- 2019/07/28 06:00
MHDA- 2019/08/10 06:00
CRDT- 2019/07/27 06:00
PHST- 2019/02/05 00:00 [received]
PHST- 2019/07/22 00:00 [accepted]
PHST- 2019/07/27 06:00 [entrez]
PHST- 2019/07/28 06:00 [pubmed]
PHST- 2019/08/10 06:00 [medline]
AID - 10.1007/s11274-019-2701-2 [pii]
AID - 10.1007/s11274-019-2701-2 [doi]
PST - epublish
SO  - World J Microbiol Biotechnol. 2019 Jul 26;35(8):122. doi: 
      10.1007/s11274-019-2701-2.