PMID- 31348766
OWN - NLM
STAT- MEDLINE
DCOM- 20200214
LR  - 20200225
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 25
DP  - 2019 Jul 26
TI  - Small Nucleolar RNA Host Gene 12 (SNHG12) Promotes Proliferation and Invasion of 
      Laryngeal Cancer Cells via Sponging miR-129-5p and Potentiating WW 
      Domain-Containing E3 Ubiquitin Protein Ligase 1 (WWP1) Expression.
PG  - 5552-5560
LID - 10.12659/MSM.917088 [doi]
AB  - BACKGROUND The clinical significance and biological function of long noncoding 
      RNA SNHG12 have not been identified in laryngeal squamous cell carcinoma (LSCC). 
      MATERIAL AND METHODS Expression levels of SNHG12, miR-129-5p, and WWP1 in LSCC 
      tissues or cells were tested by RT-qPCR. MTT assay, flow cytometry, and Transwell 
      assay were used to identify the progression of LSCC cells in vitro. Luciferase 
      reporter assay was used to assess the associations among SNHG12, WWP1, and 
      miR-129-5p. RESULTS SNHG12 was significantly overexpressed in LSCC tissues 
      compared with adjacent normal tissues. The expression level of SNHG12 was 
      significantly associated with T classification, lymph node metastasis, and cancer 
      stage of LSCC. High expression of SNHG12 predicted shorter disease-free survival. 
      Suppressing SNHG12 using siRNA inhibited proliferation and invasion and promoted 
      apoptosis in the AMC-HN-8 LSCC cell line. SNHG12, mainly located in cytoplasm of 
      AMC-HN-8 cells, was validated by dual luciferase reporter test and RT-qPCR to 
      directly interact with miR-129-5p. Inhibition of miR-129-5p significantly 
      increased proliferation and invasion of AMC-HN-8 cells and ameliorated the 
      suppressive effects of si-SNHG12. Luciferase assay showed that miR-129-5p was 
      able to combine with the 3'UTR region of WWP1, which is generally regarded as an 
      E3 ubiquitin protein ligase. RT-qPCR and Western blot showed that WWP1 was 
      positively regulated by SNHG12 and negatively regulated by miR-129-5p at the mRNA 
      level and protein level. Overexpression of WWP1 significantly increased 
      proliferation and invasion of laryngeal cancer cells. Moreover, when SNHG12 was 
      suppressed, rescue of WWP1 restored the proliferation and invasion abilities of 
      AMC-HN-8 cells. CONCLUSIONS Our study demonstrated that SNHG12 promoted LSCC 
      cells progression via sponging miR-129-5p and potentiating WWP1 expression.
FAU - Li, Jing
AU  - Li J
AD  - Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of 
      Wuhan, Tongji Medical College Huazhong University of Science and Technology, 
      Wuhan, Hubei, China (mainland).
FAU - Sun, Suguang
AU  - Sun S
AD  - Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of 
      Wuhan, Tongji Medical College Huazhong University of Science and Technology, 
      Wuhan, Hubei, China (mainland).
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of 
      Wuhan, Tongji Medical College Huazhong University of Science and Technology, 
      Wuhan, Hubei, China (mainland).
FAU - Yuan, Kun
AU  - Yuan K
AD  - Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of 
      Wuhan, Tongji Medical College Huazhong University of Science and Technology, 
      Wuhan, Hubei, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20190726
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn129 microRNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SNHG12 long non-coding RNA, human)
RN  - EC 2.3.2.26 (WWP1 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - MicroRNAs/*genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - RNA, Long Noncoding/*genetics/*metabolism
MH  - Signal Transduction
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/metabolism/pathology
MH  - Transcriptome
MH  - Ubiquitin-Protein Ligases/biosynthesis/*genetics/metabolism
PMC - PMC6681687
COIS- Conflict of interest None.
EDAT- 2019/07/28 06:00
MHDA- 2020/02/15 06:00
PMCR- 2019/07/26
CRDT- 2019/07/27 06:00
PHST- 2019/07/27 06:00 [entrez]
PHST- 2019/07/28 06:00 [pubmed]
PHST- 2020/02/15 06:00 [medline]
PHST- 2019/07/26 00:00 [pmc-release]
AID - 917088 [pii]
AID - 10.12659/MSM.917088 [doi]
PST - epublish
SO  - Med Sci Monit. 2019 Jul 26;25:5552-5560. doi: 10.12659/MSM.917088.