PMID- 31350619
OWN - NLM
STAT- MEDLINE
DCOM- 20191115
LR  - 20200225
IS  - 1432-1955 (Electronic)
IS  - 0932-0113 (Linking)
VI  - 118
IP  - 9
DP  - 2019 Sep
TI  - Histopathological characterization of Toxocara canis- and T. cati-induced 
      neurotoxocarosis in the mouse model.
PG  - 2591-2600
LID - 10.1007/s00436-019-06395-7 [doi]
AB  - Infective larvae of Toxocara canis and T. cati, the common roundworms of dogs and 
      cats, may invade the central nervous system of paratenic hosts, including humans, 
      causing neurotoxocarosis (NT). Previous studies on NT in the model organism 
      "mouse" have indicated distinct differences between T. canis and T. cati 
      regarding larval migration patterns as well as the severity of clinical symptoms 
      and behavioural alterations. The objective of the present study was to provide an 
      extensive characterization of the underlying histopathological alterations, 
      comparing T. canis- and T. cati-induced changes in different brain areas over the 
      course of murine infection. Four histological sections of five brains each of T. 
      canis- and T. cati-infected as well as uninfected C57Bl/6 mice were investigated 
      7, 14, 28, 42, 70 and 98 days post infection (dpi), while brains of T. 
      cati-infected and control mice were also available 120 and 150 dpi. In addition 
      to haematoxylin-eosin and luxol fast blue-cresyl violet staining, 
      immunohistochemistry was employed to study microglia/macrophage cell morphology 
      and to detect accumulation of beta-amyloid precursor protein (beta-APP) as an indicator 
      of axonal damage. Haemorrhages, eosinophilic vasculitis and activated 
      microglia/macrophages were detected in both infection groups starting 7 dpi, 
      followed by eosinophilic meningitis in cerebra as from 14 dpi. Overall, little 
      differences in the proportion of animals affected by these alterations were found 
      between the two infection groups. In contrast, the proportion of animals 
      displaying beta-APP accumulation was significantly higher in the T. canis than T. 
      cati group as from 28 dpi regarding the cerebrum as well as at 98 dpi regarding 
      the cerebellum. In T. canis-infected mice, myelinophagic microglia/macrophages 
      ("gitter cells") appeared as from 14 dpi, whereas these were first observed at 70 
      dpi in T. cati-infected animals. The proportion of animals displaying 
      demyelination and/or gitter cells in the cerebrum was significantly higher in the 
      T. canis than T. cati group as from 28 dpi, and at 28 and 42 dpi regarding the 
      cerebellum. Earlier and more severe neurodegeneration during T. canis- than T. 
      cati-induced NT, especially in the cerebrum, may explain the differences in 
      behavioural alterations observed in previous studies. In addition to differences 
      in larval migration preferences, immunological processes may contribute to these 
      patterns, which warrant further investigation.
FAU - Springer, Andrea
AU  - Springer A
AD  - Institute for Parasitology, Centre for Infection Medicine, University of 
      Veterinary Medicine Hannover, Buenteweg 17, 30559, Hanover, Germany.
FAU - Heuer, Lea
AU  - Heuer L
AD  - Institute for Parasitology, Centre for Infection Medicine, University of 
      Veterinary Medicine Hannover, Buenteweg 17, 30559, Hanover, Germany.
AD  - Bayer Animal Health, Alfred-Nobel-Str. 50, 40789, Monheim am Rhein, Germany.
FAU - Janecek-Erfurth, Elisabeth
AU  - Janecek-Erfurth E
AD  - Institute for Parasitology, Centre for Infection Medicine, University of 
      Veterinary Medicine Hannover, Buenteweg 17, 30559, Hanover, Germany.
AD  - Institute for Experimental Infection Research, TWINCORE, Centre for Experimental 
      and Clinical Infection Research, Hanover Medical School and the Helmholtz Centre 
      for Infection Research, 30625, Hannover, Germany.
FAU - Beineke, Andreas
AU  - Beineke A
AD  - Department of Pathology, University of Veterinary Medicine Hannover, Buenteweg 
      17, 30559, Hanover, Germany.
FAU - Strube, Christina
AU  - Strube C
AD  - Institute for Parasitology, Centre for Infection Medicine, University of 
      Veterinary Medicine Hannover, Buenteweg 17, 30559, Hanover, Germany. 
      christina.strube@tiho-hannover.de.
LA  - eng
PT  - Journal Article
DEP - 20190727
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Amyloid beta-Peptides)
SB  - IM
MH  - Amyloid beta-Peptides/metabolism
MH  - Animals
MH  - Brain/metabolism/parasitology/pathology
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Larva/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Toxocara canis/immunology/*physiology
MH  - Toxocariasis/metabolism/*parasitology/pathology
MH  - Toxoplasmosis, Cerebral/metabolism/*parasitology/pathology
OTO - NOTNLM
OT  - Demyelination
OT  - Neurodegeneration
OT  - Neurotoxocarosis
OT  - Roundworms
OT  - Zoonosis
EDAT- 2019/07/28 06:00
MHDA- 2019/11/16 06:00
CRDT- 2019/07/28 06:00
PHST- 2019/03/19 00:00 [received]
PHST- 2019/07/05 00:00 [accepted]
PHST- 2019/07/28 06:00 [pubmed]
PHST- 2019/11/16 06:00 [medline]
PHST- 2019/07/28 06:00 [entrez]
AID - 10.1007/s00436-019-06395-7 [pii]
AID - 10.1007/s00436-019-06395-7 [doi]
PST - ppublish
SO  - Parasitol Res. 2019 Sep;118(9):2591-2600. doi: 10.1007/s00436-019-06395-7. Epub 
      2019 Jul 27.