PMID- 31352112 OWN - NLM STAT- MEDLINE DCOM- 20200106 LR - 20200106 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 93 DP - 2019 Oct TI - Screening of immuno-modulatory potential of different herbal plant extracts using striped catfish (Pangasianodon hypophthalmus) leukocyte-based in vitro tests. PG - 296-307 LID - S1050-4648(19)30774-0 [pii] LID - 10.1016/j.fsi.2019.07.064 [doi] AB - Many medicinal plants have been shown to possess biological effects, including immuno-modulatory activities on human and other mammals. However, studies about the potential mechanisms of plant extracts on the humoral and tissular immunities in fish have received less attention. This study aimed to screen the immunestimulating properties of 20 ethanol plant extracts on striped catfish Pangasianodon hypophthalmus leukocytes. The peripheral blood mononuclear cells (PBMCs) and head kidney leukocytes (HKLs) of striped catfish (50 +/- 5 g per fish) were stimulated at 10 and 100 mug of each plant extract per mL of cell culture medium. Several humoral immune parameters (lysozyme, complement and total immunoglobulin) were examined at 24-h post stimulation (hps). Furthermore, the responses of four cytokine genes, namely il1beta, ifrgamma 2a and b, and mhc class II were assessed by quantitative real-time PCR at 6, 12, 24, and 48 hps. The results showed that lysozyme, complement as well as total immunoglobulin levels in both PBMCs and HKLs were regulated by some of the plant extracts tested in a concentration-dependent manner; some plant extracts induced the highest immune responses at the low dose (10 mug mL(-1)) while others were more efficient at high dose (100 mug mL(-1)). Among the extracts, five extracts including garlic Allium sativum L. (As), neem Azadirachta indica A. Juss (Ai), asthma-plant Euphorbia hirta L. (Eh), bhumi amla Phyllanthus amarus Schum. et Thonn (Pa), and ginger Zingiber officinale Rosc (Zo) induced significant changes in the expression of pro-inflammatory cytokine (il1beta), antiviral cytokines (ifrgamma 2a and b) and adaptive immune cytokine (mhc class II) in striped catfish cells. Pa always modulated the strongest expression of the four cytokines in PBMCs and HKLs over the whole experimental period (p < 0.05), whereas Zo did not stimulate the mhc class II expression in striped catfish leukocytes throughout experimental periods. These in vitro results demonstrated that some plant extracts could differently modulate great potential immune response in fish, supporting their applications in further in vivo experiments. CI - Copyright (c) 2019 Elsevier Ltd. All rights reserved. FAU - Nhu, Truong Quynh AU - Nhu TQ AD - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Rue de Bruxelles 61, B-5000, Namur, Belgium; College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: tqnhu@ctu.edu.vn. FAU - Bich Hang, Bui Thi AU - Bich Hang BT AD - College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: btbhang@ctu.edu.vn. FAU - Vinikas, Anais AU - Vinikas A AD - Pharmacognosy Research Group, Louvain Drug Research Institute, Universite Catholique de Louvain, Avenue E. Mounier, 72 bte B1. 72.03, Brussels, Belgium. Electronic address: anais.vinikas@student.uclouvain.be. FAU - Bach, Le Thi AU - Bach LT AD - College of Natural Sciences, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: ltbach@ctu.edu.vn. FAU - Buu Hue, Bui Thi AU - Buu Hue BT AD - College of Natural Sciences, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: Btbhue@ctu.edu.vn. FAU - Thanh Huong, Do Thi AU - Thanh Huong DT AD - College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: dtthuong@ctu.edu.vn. FAU - Quetin-Leclercq, Joelle AU - Quetin-Leclercq J AD - Pharmacognosy Research Group, Louvain Drug Research Institute, Universite Catholique de Louvain, Avenue E. Mounier, 72 bte B1. 72.03, Brussels, Belgium. Electronic address: joelle.leclercq@uclouvain.be. FAU - Scippo, Marie-Louise AU - Scippo ML AD - Department of Food Sciences, Laboratory of Food Analysis, Faculty of Veterinary Medicine, Fundamental and Applied Research for Animals & Health (FARAH), Veterinary Public Health, University of Liege, bat. B43bis, 10 Avenue de Cureghem, Sart-Tilman, Liege, Belgium. Electronic address: mlscippo@ulg.ac.be. FAU - Phuong, Nguyen Thanh AU - Phuong NT AD - College of Aquaculture and Fisheries, Cantho University, Campus II, Cantho City, Viet Nam. Electronic address: ntphuong@ctu.edu.vn. FAU - Kestemont, Patrick AU - Kestemont P AD - Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth and Environment (ILEE), University of Namur, Rue de Bruxelles 61, B-5000, Namur, Belgium. Electronic address: patrick.kestemont@unamur.be. LA - eng PT - Journal Article DEP - 20190725 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Immunologic Factors) RN - 0 (Plant Extracts) SB - IM MH - Animals MH - Catfishes/*immunology MH - Head Kidney/drug effects/immunology MH - Immunologic Factors/administration & dosage/*pharmacology MH - *Immunomodulation MH - Leukocytes/drug effects/*immunology MH - Leukocytes, Mononuclear/drug effects/immunology MH - Plant Extracts/administration & dosage/*pharmacology MH - Species Specificity OTO - NOTNLM OT - Ethanol plant extracts OT - Head kidney leukocytes OT - Immune system OT - Pangasianodon hypophthalmus OT - Peripheral blood mononuclear cells OT - Striped catfish EDAT- 2019/07/29 06:00 MHDA- 2020/01/07 06:00 CRDT- 2019/07/29 06:00 PHST- 2019/03/02 00:00 [received] PHST- 2019/07/16 00:00 [revised] PHST- 2019/07/24 00:00 [accepted] PHST- 2019/07/29 06:00 [pubmed] PHST- 2020/01/07 06:00 [medline] PHST- 2019/07/29 06:00 [entrez] AID - S1050-4648(19)30774-0 [pii] AID - 10.1016/j.fsi.2019.07.064 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2019 Oct;93:296-307. doi: 10.1016/j.fsi.2019.07.064. Epub 2019 Jul 25.