PMID- 31354164
OWN - NLM
STAT- MEDLINE
DCOM- 20200214
LR  - 20200225
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 25
DP  - 2019 Jul 29
TI  - Emodin Alleviates the Airway Inflammation of Cough Variant Asthma in Mice by 
      Regulating the Notch Pathway.
PG  - 5621-5629
LID - 10.12659/MSM.915080 [doi]
AB  - BACKGROUND This study investigated the effects and underlying mechanisms of 
      emodin on cough variant asthma (CVA) in mice. MATERIAL AND METHODS The bronchial 
      asthma mouse model was successfully established by use of ovalbumin (OVA) 
      sensitization and challenge. The BALB/c mice were divided into 6 groups: a 
      control group, an OVA model without or with emodin (15, 30, 60 mg/kg) group, and 
      a dexamethasone (0.5 mg/g) group. The effect of the treatment was determined by 
      measuring airway responsiveness. The levels of immunoglobulin molecules, as well 
      as inflammatory cytokines in bronchoalveolar lavage fluid (BALF) and serum, were 
      determined by ELISA. The lung tissues were stained by hematoxylin-eosin (HE). The 
      expressions of Notch receptors (Notch 1-3) and Delta-like (DLL) 4 in the lung 
      tissues were detected by RT-PCR and Western blot analysis. RESULTS Compared with 
      the model group, emodin treatment significantly increased the levels of 
      immunoglobulin E (IgE) and IgG1/IgG2a in BALF and serum (p<0.05). HE results 
      indicated that emodin inhibited the infiltration of inflammatory cells and that 
      emodin reduced the levels of inflammatory cytokines, interleukin (IL)-5, IL-17, 
      and interferon (IFN)-gamma in BALF and serum (p<0.05). Furthermore, the expressions 
      of Notch 1, 2, 3, and DLL4 in lung tissue were inhibited by emodin treatment. 
      CONCLUSIONS The results demonstrated that emodin alleviated inflammation in CVA 
      mice, which might be associated with suppression of the Notch pathway. Emodin 
      might be a promising therapeutic agent for allergic asthma.
FAU - Hua, Shu
AU  - Hua S
AD  - Department of Pediatrics, Yantai Hospital of Traditional of Chinese Medicine, 
      Yantai, Shandong, China (mainland).
FAU - Liu, Fengai
AU  - Liu F
AD  - Department of Pediatrics, Haiyang People's Hospital, Haiyang, Shandong, China 
      (mainland).
FAU - Wang, Manman
AU  - Wang M
AD  - Department of Pediatrics, Zhejiang Xiaoshan Hospital, Hangzhou, Zhejiang, China 
      (mainland).
LA  - eng
PT  - Journal Article
DEP - 20190729
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Notch)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - KA46RNI6HN (Emodin)
SB  - IM
MH  - Animals
MH  - Asthma/*drug therapy/metabolism/pathology
MH  - Bronchoalveolar Lavage Fluid
MH  - Cough/drug therapy/pathology
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Emodin/*pharmacology
MH  - Eosinophils/metabolism
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Inflammation/metabolism
MH  - Lung/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/pharmacology
MH  - Receptors, Notch/*metabolism
PMC - PMC6685324
COIS- Conflict of interests None.
EDAT- 2019/07/30 06:00
MHDA- 2020/02/15 06:00
PMCR- 2019/07/29
CRDT- 2019/07/30 06:00
PHST- 2019/07/30 06:00 [entrez]
PHST- 2019/07/30 06:00 [pubmed]
PHST- 2020/02/15 06:00 [medline]
PHST- 2019/07/29 00:00 [pmc-release]
AID - 915080 [pii]
AID - 10.12659/MSM.915080 [doi]
PST - epublish
SO  - Med Sci Monit. 2019 Jul 29;25:5621-5629. doi: 10.12659/MSM.915080.