PMID- 31355218
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 6
DP  - 2019
TI  - Synthetic Cationic Peptide IDR-1002 and Human Cathelicidin LL37 Modulate the Cell 
      Innate Response but Differentially Impact PRRSV Replication in vitro.
PG  - 233
LID - 10.3389/fvets.2019.00233 [doi]
LID - 233
AB  - Host defense peptides (HDPs) show both antimicrobial and immunomodulatory 
      properties making them important mediators of the host immune system. In humans 
      but also in pigs many HDPs have been identified and important families such as 
      cathelicidins and defensins have been established. In our study, we assessed: (i) 
      the potential interactions that could occur between three peptides (LL37, PR39, 
      and synthetic innate defense regulator (IDR)-1002) and a common TLR ligand called 
      poly(I:C); (ii) the impact of selected peptides on the response of alveolar 
      macrophage (AM) to poly(I:C) stimulation; (iii) the anti-porcine respiratory and 
      reproductive syndrome virus (PRRSV) properties of the peptides; and (iv) their 
      adjuvant potential in a PRRSV challenge experiment after immunization with 
      different vaccine formulations. The results are as following: LL37, PR39, and 
      IDR-1002 were able to interact with poly(I:C) using an agarose gel migration 
      assay. Then, an alteration of AM's response to poly(I:C) stimulation was observed 
      when the cells were co-stimulated with LL37 and IDR-1002. Regarding the 
      anti-PRRSV potential of the peptides only LL37 showed a PRRSV inhibition in 
      infected AM as well as precision cut lung slices (PCLS). However, in our 
      conditions and despite their immunomodulatory properties, neither LL37 nor 
      IDR-1002 showed any convincing potential as an adjuvant when associated to killed 
      PRRSV in a challenge experiment. In conclusion, both antiviral and 
      immunomodulatory properties could be identified for LL37, only immunomodulatory 
      properties for IDR-1002, and both peptides failed to improve the immune response 
      consecutive to an immunization with a killed vaccine in a PPRSV challenge 
      experiment. However, further studies are needed to fully decipher and explain 
      differences between peptide properties.
FAU - Levast, Benoit
AU  - Levast B
AD  - Vaccine and Infectious Disease Organization-International Vaccine Centre, 
      University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Hogan, Daniel
AU  - Hogan D
AD  - Department of Computer Science, University of Saskatchewan, Saskatoon, SK, 
      Canada.
FAU - van Kessel, Jill
AU  - van Kessel J
AD  - Vaccine and Infectious Disease Organization-International Vaccine Centre, 
      University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Strom, Stacy
AU  - Strom S
AD  - Vaccine and Infectious Disease Organization-International Vaccine Centre, 
      University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Walker, Stew
AU  - Walker S
AD  - Vaccine and Infectious Disease Organization-International Vaccine Centre, 
      University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Zhu, Jianzhong
AU  - Zhu J
AD  - College of Veterinary Medicine, Comparative Medicine Research Institute, Yangzhou 
      University, Yangzhou, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou, China.
FAU - Meurens, Francois
AU  - Meurens F
AD  - BIOEPAR, INRA, Oniris, Nantes, France.
FAU - Gerdts, Volker
AU  - Gerdts V
AD  - Vaccine and Infectious Disease Organization-International Vaccine Centre, 
      University of Saskatchewan, Saskatoon, SK, Canada.
AD  - Department of Veterinary Microbiology and Immunology, Western College of 
      Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
LA  - eng
PT  - Journal Article
DEP - 20190712
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC6640542
OTO - NOTNLM
OT  - PRRSV
OT  - adjuvant
OT  - host defense peptides
OT  - poly(I:C)
OT  - porcine alveolar macrophages
EDAT- 2019/07/30 06:00
MHDA- 2019/07/30 06:01
PMCR- 2019/01/01
CRDT- 2019/07/30 06:00
PHST- 2019/02/16 00:00 [received]
PHST- 2019/06/27 00:00 [accepted]
PHST- 2019/07/30 06:00 [entrez]
PHST- 2019/07/30 06:00 [pubmed]
PHST- 2019/07/30 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2019.00233 [doi]
PST - epublish
SO  - Front Vet Sci. 2019 Jul 12;6:233. doi: 10.3389/fvets.2019.00233. eCollection 
      2019.