PMID- 31356818
OWN - NLM
STAT- MEDLINE
DCOM- 20200904
LR  - 20200904
IS  - 1096-0805 (Electronic)
IS  - 0022-2011 (Linking)
VI  - 166
DP  - 2019 Sep
TI  - First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina.
PG  - 107222
LID - S0022-2011(18)30350-1 [pii]
LID - 10.1016/j.jip.2019.107222 [doi]
AB  - Ostreid herpesvirus 1 (OsHV-1) is a DNA virus of the genus Ostreavirus 
      (Malacoherpesviridae family, Herpesvirales order). Worldwide, OsHV-1 and its 
      microvariants have been associated with increased mortality of Pacific oysters, 
      Crassostrea gigas. Adult asymptomatic oysters also have shown a high prevalence 
      of viral infection. As a consequence, surveillance is needed to better describe 
      OsHV-1 diversity, pathogenicity, clinical signs, and geographical distribution. 
      We examined Crassostrea gigas sampled in October 2017 from the inner zone of the 
      Bahia Blanca Estuary, Argentina, and found that 8 of 30 specimens (26.7%) 
      presented macroscopic lesions in mantle tissues. Histological analysis revealed 
      abnormal presentation of mantle epithelial cells and connective tissues. 
      Conventional and real-time PCR conducted on the oyster samples revealed 70% to be 
      positive for presence of OsHV-1 DNA. The nucleotide sequence of the amplicon 
      obtained from one sample using the primer pair IA1/IA2 (targeting ORF 42/43) was 
      99% identical to OsHV-1 reference as well as microVar strains B and A (KY271630, 
      KY242785.1), sequenced from France and Ireland. This finding represents the first 
      detection of OsHV-1 DNA in a wild population of C. gigas in Argentina in 
      association with gross mantle lesions.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Barbieri, Elena S
AU  - Barbieri ES
AD  - CESIMAR-CONICET, Laboratorio de Oceanografia Biologica, Boulevard Brown 2915, 
      Puerto Madryn (U9120ACD), Chubut, Argentina. Electronic address: 
      barbieri@cenpat-conicet.gob.ar.
FAU - Medina, Cintia D
AU  - Medina CD
AD  - UAT CCT CONICET-CENPAT, Laboratorio de Genetica Molecular, Boulevard Brown 2915, 
      Puerto Madryn (U9120ACD), Chubut, Argentina.
FAU - Vazquez, Nuria
AU  - Vazquez N
AD  - IBIOMAR-CONICET, Laboratorio de Parasitologia, Boulevard Brown 2915, Puerto 
      Madryn (U9120ACD), Chubut, Argentina.
FAU - Fiorito, Carla
AU  - Fiorito C
AD  - CESIMAR-CONICET, Grupo de Ecofisiologia Aplicada, Boulevard Brown 2915, Puerto 
      Madryn (U9120ACD), Chubut, Argentina.
FAU - Martelli, Antonela
AU  - Martelli A
AD  - CESIMAR-CONICET, Laboratorio de Oceanografia Biologica, Boulevard Brown 2915, 
      Puerto Madryn (U9120ACD), Chubut, Argentina.
FAU - Wigdorovitz, Andres
AU  - Wigdorovitz A
AD  - CICVyA, Laboratorio de IncuINTA, Nicolas Repetto y de los Reseros s/n, (1686) 
      Hurlingham, Buenos Aires, Argentina.
FAU - Schwindt, Evangelina
AU  - Schwindt E
AD  - IBIOMAR-CONICET, Grupo de Ecologia en Ambientes Costeros, Boulevard Brown 2915, 
      Puerto Madryn (U9120ACD), Chubut, Argentina.
FAU - Morga, Benjamin
AU  - Morga B
AD  - IFREMER, SG2M-LGPMM, Laboratoire de Genetique et Pathologie des Mollusques 
      Marins, La Tremblade, France.
FAU - Renault, Tristan
AU  - Renault T
AD  - IFREMER, Departement Ressources Biologiques et Environnement, Nantes, France.
FAU - Parreno, Viviana
AU  - Parreno V
AD  - CICVyA, Laboratorio de IncuINTA, Nicolas Repetto y de los Reseros s/n, (1686) 
      Hurlingham, Buenos Aires, Argentina.
FAU - Baron, Pedro J
AU  - Baron PJ
AD  - CESIMAR-CONICET, Laboratorio de Oceanografia Biologica, Boulevard Brown 2915, 
      Puerto Madryn (U9120ACD), Chubut, Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190726
PL  - United States
TA  - J Invertebr Pathol
JT  - Journal of invertebrate pathology
JID - 0014067
RN  - 0 (DNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Argentina
MH  - Crassostrea/*virology
MH  - DNA Viruses/*genetics
MH  - DNA, Viral/analysis
MH  - Introduced Species
MH  - Phylogeny
MH  - Shellfish/*virology
OTO - NOTNLM
OT  - Argentina
OT  - Crassostrea gigas
OT  - Ostreid herpesvirus 1
OT  - Pacific oyster
EDAT- 2019/07/30 06:00
MHDA- 2020/09/05 06:00
CRDT- 2019/07/30 06:00
PHST- 2018/11/08 00:00 [received]
PHST- 2019/07/23 00:00 [revised]
PHST- 2019/07/25 00:00 [accepted]
PHST- 2019/07/30 06:00 [pubmed]
PHST- 2020/09/05 06:00 [medline]
PHST- 2019/07/30 06:00 [entrez]
AID - S0022-2011(18)30350-1 [pii]
AID - 10.1016/j.jip.2019.107222 [doi]
PST - ppublish
SO  - J Invertebr Pathol. 2019 Sep;166:107222. doi: 10.1016/j.jip.2019.107222. Epub 
      2019 Jul 26.