PMID- 31356836
OWN - NLM
STAT- MEDLINE
DCOM- 20201019
LR  - 20201019
IS  - 1872-678X (Electronic)
IS  - 0165-0270 (Linking)
VI  - 326
DP  - 2019 Oct 1
TI  - Single-cell memory trace imaging with immediate-early genes.
PG  - 108368
LID - S0165-0270(19)30225-0 [pii]
LID - 10.1016/j.jneumeth.2019.108368 [doi]
AB  - For the past decades, an increasing number of studies has taken advantage of 
      molecular imaging methods involving the detection of immediate-early genes' 
      (IEGs) expression for investigating neural substrates underlying plasticity 
      processes and memory function. The detection of IEGs RNA by Fluorescent In-Situ 
      Hybridization (FISH) yields single-cell as well as high temporal resolution and 
      has recently enabled the mapping of medial temporal lobe subareas/subnetworks 
      activity induced by single or multiple behavioural events in the same animal. 
      After briefly reviewing the function and the ties of the typical IEGs (Fos, 
      Zif268, Arc, Homer1a) used for mapping plasticity, we focus on discussing 
      technical considerations vital for the successful detection of IEGs with FISH 
      with emphasis on the design of RNA probes, the optimization of experimental 
      conditions and the necessity for controls. Finally, we discuss recent 
      developments in brain clearing methods that in combination with FISH detection of 
      IEGs' expression allow for 3D imaging with single cell resolution as well as 
      whole brain analyses. This, in parallel with the recent development of fMRI 
      cognitive tasks in awake rats and the use of high resolution fMRI in humans, 
      holds great promises for bridging further memory in humans and animals.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Sauvage, Magdalena
AU  - Sauvage M
AD  - Leibniz-Institute for Neurobiology, Functional Architecture of Memory Dept., 
      39118, Magdeburg, Germany; Otto von Guericke University, Medical Faculty, 
      Functional Neuroplasticity Dept., 39120, Magdeburg, Germany; Otto von Guericke 
      University, Center for Behavioral Brain Sciences, 39106, Magdeburg, Germany. 
      Electronic address: magdalena.sauvage@lin-magdeburg.de.
FAU - Kitsukawa, Takashi
AU  - Kitsukawa T
AD  - Graduate school of Frontier Biosciences, Osaka University, Suita, Osaka, 
      565-0871, Japan.
FAU - Atucha, Erika
AU  - Atucha E
AD  - Leibniz-Institute for Neurobiology, Functional Architecture of Memory Dept., 
      39118, Magdeburg, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20190726
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
SB  - IM
MH  - Animals
MH  - Brain/*physiology
MH  - Gene Expression/*physiology
MH  - Genes, Immediate-Early/*physiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Memory/*physiology
MH  - Neuronal Plasticity/*physiology
MH  - Neurosciences/*methods
MH  - Single-Cell Analysis/*methods
OTO - NOTNLM
OT  - Arc
OT  - Catfish
OT  - FISH
OT  - IEG
OT  - Immediate-early-gene
OT  - Memory
EDAT- 2019/07/30 06:00
MHDA- 2020/10/21 06:00
CRDT- 2019/07/30 06:00
PHST- 2019/05/29 00:00 [received]
PHST- 2019/07/15 00:00 [revised]
PHST- 2019/07/17 00:00 [accepted]
PHST- 2019/07/30 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/07/30 06:00 [entrez]
AID - S0165-0270(19)30225-0 [pii]
AID - 10.1016/j.jneumeth.2019.108368 [doi]
PST - ppublish
SO  - J Neurosci Methods. 2019 Oct 1;326:108368. doi: 10.1016/j.jneumeth.2019.108368. 
      Epub 2019 Jul 26.