PMID- 31356943
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 139
DP  - 2019 Oct 15
TI  - Identification of a linear B-cell epitope on glycoprotein (GP) 2a of porcine 
      reproductive and respiratory syndrome virus (PRRSV).
PG  - 1288-1294
LID - S0141-8130(19)33843-7 [pii]
LID - 10.1016/j.ijbiomac.2019.07.171 [doi]
AB  - Glycoprotein (GP) 2a was a minor structural protein of porcine reproductive and 
      respiratory syndrome virus (PRRSV) and was one of crucial proteins for PRRSV to 
      bind cell receptor, which indicated that there were neutralizing epitopes on 
      GP2a. In the present work, we used mouse anti-GP2a(41-208aa) serum and one 
      GP2a(41-208aa) specific monoclonal antibody (McAb) to identify B-cell epitopes of 
      GP2a by peptide-based ELISA. A liner B-cell epitope F(194)PTPGSRPKLHDFQQ(208) was 
      identified. However, the results of virus neutralization experiment showed that 
      the McAb could not reduce the titers of PRRSV, which indicated that the 
      identified epitope was not the neutralizing epitope of PRRSV. While the amino 
      acid sequence of this epitope was conserved in North American (type 2) PRRSV, 
      which suggested that this epitope might be diagnostic potential for type 2 PRRSV 
      strains. In conclusion, our present work identified a new epitope on GP2a and 
      this epitope might be diagnostic potential for type 2 PRRSV strains.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - Shi, Xibao
AU  - Shi X
AD  - College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China.
FAU - Fan, Xiaomin
AU  - Fan X
AD  - College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China.
FAU - Nie, Shouyi
AU  - Nie S
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan 450000, 
      China.
FAU - Kou, Liyun
AU  - Kou L
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan 450000, 
      China.
FAU - Zhang, Xiaozhuan
AU  - Zhang X
AD  - College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China.
FAU - Liu, Hongliang
AU  - Liu H
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan 450000, 
      China.
FAU - Ji, Shengdong
AU  - Ji S
AD  - College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China.
FAU - Deng, Ruiguang
AU  - Deng R
AD  - Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of 
      Agricultural Sciences, Zhengzhou 450002, China.
FAU - Wang, Aiping
AU  - Wang A
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan 450000, 
      China. Electronic address: pingaw@126.com.
FAU - Zhang, Gaiping
AU  - Zhang G
AD  - Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan 450000, 
      China; College of Animal Science and Veterinary Medicine, Henan Agricultural 
      University, Zhengzhou, Henan 450002, China; Henan Provincial Key Laboratory of 
      Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, 
      China. Electronic address: zhanggaiping2003@163.com.
LA  - eng
PT  - Journal Article
DEP - 20190726
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Conserved Sequence
MH  - Epitope Mapping
MH  - Epitopes, B-Lymphocyte/*immunology
MH  - Mice
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Sequence Alignment
MH  - Viral Envelope Proteins/chemistry/*immunology
OTO - NOTNLM
OT  - Epitope
OT  - GP2a
OT  - PRRSV
EDAT- 2019/07/30 06:00
MHDA- 2020/04/09 06:00
CRDT- 2019/07/30 06:00
PHST- 2019/05/23 00:00 [received]
PHST- 2019/07/12 00:00 [revised]
PHST- 2019/07/25 00:00 [accepted]
PHST- 2019/07/30 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/07/30 06:00 [entrez]
AID - S0141-8130(19)33843-7 [pii]
AID - 10.1016/j.ijbiomac.2019.07.171 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Oct 15;139:1288-1294. doi: 
      10.1016/j.ijbiomac.2019.07.171. Epub 2019 Jul 26.