PMID- 31358509
OWN - NLM
STAT- MEDLINE
DCOM- 20200305
LR  - 20201125
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 6
IP  - 4
DP  - 2019 Jul/Aug
TI  - Interneuron Functional Diversity in the Mouse Accessory Olfactory Bulb.
LID - ENEURO.0058-19.2019 [pii]
LID - 10.1523/ENEURO.0058-19.2019 [doi]
AB  - In the mouse accessory olfactory bulb (AOB), inhibitory interneurons play an 
      essential role in gating behaviors elicited by sensory exposure to social odors. 
      Several morphological classes have been described, but the full complement of 
      interneurons remains incomplete. In order to develop a more comprehensive view of 
      interneuron function in the AOB, we performed targeted patch clamp recordings 
      from partially overlapping subsets of genetically labeled and morphologically 
      defined interneuron types. Gad2 (GAD65), Calb2 (calretinin), and Cort 
      (cortistatin)-cre mouse lines were used to drive selective expression of tdTomato 
      in AOB interneurons. Gad2 and Calb2-labeled interneurons were found in the 
      internal, external, and glomerular (GL) layers, whereas Cort-labeled interneurons 
      were enriched within the lateral olfactory tract (LOT) and external cellular 
      layer (ECL). We found that external granule cells (EGCs) from all genetically 
      labeled subpopulations possessed intrinsic functional differences that allowed 
      them to be readily distinguished from internal granule cells (IGCs). EGCs showed 
      stronger voltage-gated Na(+) and non-inactivating voltage-gated K(+) currents, 
      decreased I(H) currents, and robust excitatory synaptic input. These specific 
      intrinsic properties did not correspond to any genetically labeled type, 
      suggesting that transcriptional heterogeneity among EGCs and IGCs is not 
      correlated with expression of these particular marker genes. Intrinsic 
      heterogeneity was also seen among AOB juxtaglomerular cells (JGCs), with a major 
      subset of Calb2-labeled JGCs exhibiting spontaneous and depolarization-evoked 
      plateau potentials. These data identify specific physiological features of AOB 
      interneurons types that will assist in future studies of AOB function.
CI  - Copyright (c) 2019 Maksimova et al.
FAU - Maksimova, Marina A
AU  - Maksimova MA
AD  - Department of Neuroscience, University of Texas Southwestern Medical Center, 
      Dallas, TX 75390.
FAU - Cansler, Hillary L
AU  - Cansler HL
AUID- ORCID: 0000-0001-8267-0515
AD  - Department of Neuroscience, University of Texas Southwestern Medical Center, 
      Dallas, TX 75390.
FAU - Zuk, Kelsey E
AU  - Zuk KE
AD  - Department of Neuroscience, University of Texas Southwestern Medical Center, 
      Dallas, TX 75390.
FAU - Torres, Jennifer M
AU  - Torres JM
AD  - Department of Neuroscience, University of Texas Southwestern Medical Center, 
      Dallas, TX 75390.
FAU - Roberts, Dylan J
AU  - Roberts DJ
AD  - Department of Neuroscience, University of Texas Southwestern Medical Center, 
      Dallas, TX 75390.
FAU - Meeks, Julian P
AU  - Meeks JP
AUID- ORCID: 0000-0002-7537-4491
AD  - Department of Neuroscience, University of Texas Southwestern Medical Center, 
      Dallas, TX 75390 julian.meeks@utsouthwestern.edu.
AD  - Department of Neurology and Neurotherapeutics, University of Texas Southwestern 
      Medical Center, Dallas, TX 75390.
LA  - eng
GR  - R01 DC015784/DC/NIDCD NIH HHS/United States
GR  - R21 NS104826/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190813
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Calb2 protein, mouse)
RN  - 0 (Calbindin 2)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 2)
SB  - IM
MH  - Animals
MH  - Calbindin 2/metabolism
MH  - Female
MH  - Glutamate Decarboxylase/metabolism
MH  - Interneurons/cytology/metabolism/*physiology
MH  - Male
MH  - Mice, Transgenic
MH  - Olfactory Bulb/cytology/metabolism/*physiology
MH  - Synaptic Potentials
PMC - PMC6712203
OTO - NOTNLM
OT  - accessory olfactory bulb
OT  - cell types
OT  - excitability
OT  - interneuron
EDAT- 2019/07/31 06:00
MHDA- 2020/03/07 06:00
PMCR- 2019/08/13
CRDT- 2019/07/31 06:00
PHST- 2019/02/15 00:00 [received]
PHST- 2019/07/18 00:00 [revised]
PHST- 2019/07/19 00:00 [accepted]
PHST- 2019/07/31 06:00 [pubmed]
PHST- 2020/03/07 06:00 [medline]
PHST- 2019/07/31 06:00 [entrez]
PHST- 2019/08/13 00:00 [pmc-release]
AID - ENEURO.0058-19.2019 [pii]
AID - eN-NWR-0058-19 [pii]
AID - 10.1523/ENEURO.0058-19.2019 [doi]
PST - epublish
SO  - eNeuro. 2019 Aug 13;6(4):ENEURO.0058-19.2019. doi: 10.1523/ENEURO.0058-19.2019. 
      Print 2019 Jul/Aug.