PMID- 31359237
OWN - NLM
STAT- MEDLINE
DCOM- 20200512
LR  - 20240421
IS  - 2509-2723 (Electronic)
IS  - 2509-2715 (Print)
IS  - 2509-2723 (Linking)
VI  - 41
IP  - 4
DP  - 2019 Aug
TI  - Effect of caloric restriction and rapamycin on ovarian aging in mice.
PG  - 395-408
LID - 10.1007/s11357-019-00087-x [doi]
AB  - Caloric restriction (CR) increases the preservation of the ovarian primordial 
      follicular reserve, which can potentially delay menopause. Rapamycin also 
      increases preservation on the ovarian reserve, with similar mechanism to CR. 
      Therefore, the aim of our study was to evaluate the effects of rapamycin and CR 
      on metabolism, ovarian reserve, and gene expression in mice. Thirty-six female 
      mice were allocated into three groups: control, rapamycin-treated (4 mg/kg body 
      weight every other day), and 30% CR. Caloric restricted females had lower body 
      weight (P < 0.05) and increased insulin sensitivity (P = 0.003), while rapamycin 
      injection did not change body weight (P > 0.05) and induced insulin resistance 
      (P < 0.05). Both CR and rapamycin females displayed a higher number of primordial 
      follicles (P = 0.02 and 0.04, respectively), fewer primary, secondary, and 
      tertiary follicles (P < 0.05) and displayed increased ovarian Foxo3a gene 
      expression (P < 0.05). Despite the divergent metabolic effects of the CR and 
      rapamycin treatments, females from both groups displayed a similar increase in 
      ovarian reserve, which was associated with higher expression of ovarian Foxo3a.
FAU - Garcia, Driele N
AU  - Garcia DN
AD  - Faculdade de Nutricao, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
FAU - Saccon, Tatiana D
AU  - Saccon TD
AD  - Faculdade de Nutricao, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
FAU - Pradiee, Jorgea
AU  - Pradiee J
AD  - Faculdade de Medicina Veterinaria, Universidade Federal de Pelotas, Pelotas, RS, 
      Brazil.
FAU - Rincon, Joao A A
AU  - Rincon JAA
AD  - Faculdade de Medicina Veterinaria, Universidade Federal de Pelotas, Pelotas, RS, 
      Brazil.
FAU - Andrade, Kelvin R S
AU  - Andrade KRS
AD  - Faculdade de Nutricao, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
FAU - Rovani, Monique T
AU  - Rovani MT
AD  - Faculdade de Medicina Veterinaria, Universidade Federal de Pelotas, Pelotas, RS, 
      Brazil.
FAU - Mondadori, Rafael G
AU  - Mondadori RG
AD  - Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
FAU - Cruz, Luis A X
AU  - Cruz LAX
AD  - Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
FAU - Barros, Carlos C
AU  - Barros CC
AD  - Faculdade de Nutricao, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
FAU - Masternak, Michal M
AU  - Masternak MM
AD  - College of Medicine, Burnett School of Biomedical Sciences, University of Central 
      Florida, Orlando, FL, USA.
AD  - Department of Head and Neck Surgery, The Greater Poland Cancer Centre, Poznan, 
      Poland.
FAU - Bartke, Andrzej
AU  - Bartke A
AD  - Departments of Internal Medicine and Physiology, Southern Illinois University 
      School of Medicine, Springfield, IL, USA.
FAU - Mason, Jeffrey B
AU  - Mason JB
AD  - Department of Animal, Dairy and Veterinary Sciences, Center for Integrated 
      BioSystems, School of Veterinary Medicine, Utah State University, Logan, UT, USA.
FAU - Schneider, Augusto
AU  - Schneider A
AUID- ORCID: 0000-0002-3410-2860
AD  - Faculdade de Nutricao, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
LA  - eng
GR  - R03 AG059846/AG/NIA NIH HHS/United States
GR  - R15 AG059190/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190729
PL  - Switzerland
TA  - Geroscience
JT  - GeroScience
JID - 101686284
RN  - 0 (Forkhead Box Protein O3)
RN  - 0 (FoxO3 protein, mouse)
RN  - 0 (Immunosuppressive Agents)
RN  - 63231-63-0 (RNA)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - *Caloric Restriction
MH  - Female
MH  - Forkhead Box Protein O3/genetics/metabolism
MH  - Gene Expression
MH  - Immunosuppressive Agents/*pharmacology
MH  - Insulin Resistance
MH  - Mice, Inbred C57BL
MH  - Ovarian Follicle/*pathology
MH  - *Ovarian Reserve
MH  - Ovary/metabolism
MH  - RNA/metabolism
MH  - Sirolimus/*pharmacology
PMC - PMC6815295
OTO - NOTNLM
OT  - FOXO3a
OT  - Ovarian reserve
OT  - Rapamycin
OT  - mTOR
COIS- The authors declare that they have no conflict of interest.
EDAT- 2019/07/31 06:00
MHDA- 2020/05/19 06:00
PMCR- 2019/07/29
CRDT- 2019/07/31 06:00
PHST- 2019/06/07 00:00 [received]
PHST- 2019/07/16 00:00 [accepted]
PHST- 2019/07/31 06:00 [pubmed]
PHST- 2020/05/19 06:00 [medline]
PHST- 2019/07/31 06:00 [entrez]
PHST- 2019/07/29 00:00 [pmc-release]
AID - 10.1007/s11357-019-00087-x [pii]
AID - 87 [pii]
AID - 10.1007/s11357-019-00087-x [doi]
PST - ppublish
SO  - Geroscience. 2019 Aug;41(4):395-408. doi: 10.1007/s11357-019-00087-x. Epub 2019 
      Jul 29.