PMID- 31359753
OWN - NLM
STAT- MEDLINE
DCOM- 20200928
LR  - 20200928
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 91
IP  - 17
DP  - 2019 Sep 3
TI  - Chemical Changes On, and Through, The Bacterial Envelope in Escherichia coli 
      Mutants Exhibiting Impaired Plasmid Transfer Identified Using Time-of-Flight 
      Secondary Ion Mass Spectrometry.
PG  - 11355-11361
LID - 10.1021/acs.analchem.9b02533 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a (CO(2))(6k)(+) 
      gas cluster ion beam (GCIB) was used to analyze Escherichia coli mutants 
      previously identified as having impaired plasmid transfer capability related to 
      the spread of antibiotic resistance. The subset of mutants selected were expected 
      to result in changes in the bacterial envelope composition through the deletion 
      of genes encoding for FabF, DapF, and Lpp, where the surface sensitivity of 
      ToF-SIMS can be most useful. Analysis of arrays of spotted bacteria allowed 
      changes in the lipid composition of the bacteria to be elucidated using 
      multivariate analysis and confirmed through imaging of individual ion signals. 
      Significant changes in chemical composition were observed, including a surprising 
      loss of cyclopropanated fatty acids in the fabF mutant where FabF is associated 
      with the elongation of FA(16:1) to FA(18:1) and not cyclopropane formation. The 
      ability of the GCIB to generate increased higher mass signals from biological 
      samples allowed intact lipid A (m/z 1796) to be detected on the bacteria and, 
      despite a 40 keV impact energy, depth profiled through the bacterial envelope 
      along with other high mass ions including species at m/z 1820 and 2428, 
      attributed to ECA(CYC), that were only observed below the surface of the bacteria 
      and were notably absent in the depth profile of the lpp mutant. The analysis 
      provides new insights into the action of the specific pathways targeted in this 
      study and paves the way for whole new avenues for the characterization of intact 
      molecules within the bacterial envelope.
FAU - Dimovska Nilsson, Kelly
AU  - Dimovska Nilsson K
AD  - Department of Chemistry and Molecular Biology , University of Gothenburg , 
      Gothenburg 405 30 , Sweden.
FAU - Palm, Martin
AU  - Palm M
AD  - Department of Chemistry and Molecular Biology , University of Gothenburg , 
      Gothenburg 405 30 , Sweden.
AD  - Centre for Antibiotic Resistance Research , University of Gothenburg , Gothenburg 
      405 30 , Sweden.
FAU - Hood, James
AU  - Hood J
AD  - School of Engineering , Newcastle University , Newcastle upon Tyne NE1 7RU , 
      United Kingdom.
FAU - Sheriff, Jake
AU  - Sheriff J
AD  - School of Engineering , Newcastle University , Newcastle upon Tyne NE1 7RU , 
      United Kingdom.
FAU - Farewell, Anne
AU  - Farewell A
AD  - Department of Chemistry and Molecular Biology , University of Gothenburg , 
      Gothenburg 405 30 , Sweden.
AD  - Centre for Antibiotic Resistance Research , University of Gothenburg , Gothenburg 
      405 30 , Sweden.
FAU - Fletcher, John S
AU  - Fletcher JS
AUID- ORCID: 0000-0002-9418-8571
AD  - Department of Chemistry and Molecular Biology , University of Gothenburg , 
      Gothenburg 405 30 , Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190814
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (Lipid A)
RN  - 0 (Lipids)
RN  - 0 (Lipoproteins)
RN  - 0 (Lpp protein, E coli)
RN  - 0 (Mutant Proteins)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.179 (fabF protein, E coli)
RN  - EC 5.1.1.- (Amino Acid Isomerases)
RN  - EC 5.1.1.7 (DapF protein, E coli)
RN  - EC 6.- (Fatty Acid Synthase, Type II)
SB  - IM
MH  - Acetyltransferases/genetics
MH  - Amino Acid Isomerases/genetics
MH  - Bacterial Outer Membrane Proteins/genetics
MH  - Cell Membrane/*chemistry
MH  - Drug Resistance, Microbial
MH  - Escherichia coli/chemistry/enzymology/*genetics/ultrastructure
MH  - Escherichia coli Proteins/genetics
MH  - Fatty Acid Synthase, Type II/genetics
MH  - Fatty Acids/analysis
MH  - Lipid A/analysis
MH  - Lipids/analysis
MH  - Lipoproteins/genetics
MH  - Mutant Proteins
MH  - Plasmids/*metabolism
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2019/07/31 06:00
MHDA- 2020/09/29 06:00
CRDT- 2019/07/31 06:00
PHST- 2019/07/31 06:00 [pubmed]
PHST- 2020/09/29 06:00 [medline]
PHST- 2019/07/31 06:00 [entrez]
AID - 10.1021/acs.analchem.9b02533 [doi]
PST - ppublish
SO  - Anal Chem. 2019 Sep 3;91(17):11355-11361. doi: 10.1021/acs.analchem.9b02533. Epub 
      2019 Aug 14.