PMID- 31360699
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231107
IS  - 2313-433X (Print)
IS  - 2313-433X (Electronic)
IS  - 2313-433X (Linking)
VI  - 5
IP  - 7
DP  - 2019 Jul
TI  - Imaging Membrane Curvature inside a FcepsilonRI-Centric Synapse in RBL-2H3 Cells Using 
      TIRF Microscopy with Polarized Excitation.
LID - 10.3390/jimaging5070063 [doi]
LID - 63
AB  - Total internal reflection fluorescence microscopy with polarized excitation 
      (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We 
      used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed 
      with fluorescent anti-dinitrophenyl (anti-DNP) immunoglobulin E (IgE) coming into 
      contact with a supported lipid bilayer containing mobile, monovalent DNP, 
      modeling an immunological synapse. The spatial relationship of the IgE-bound high 
      affinity IgE receptor (FcepsilonRI) to the ratio image of P-polarized excitation and 
      S-polarized excitation was analyzed. These studies help correlate the dynamics of 
      cell surface molecules with the mechanical properties of the plasma membrane 
      during synapse formation.
FAU - Machado, Rosa
AU  - Machado R
AD  - UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado 
      Springs, Colorado Springs, CO 80918, USA.
FAU - Bendesky, Justin
AU  - Bendesky J
AD  - UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado 
      Springs, Colorado Springs, CO 80918, USA.
FAU - Brown, Madison
AU  - Brown M
AD  - UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado 
      Springs, Colorado Springs, CO 80918, USA.
AD  - Department of Physics and Energy Science, University of Colorado at Colorado 
      Springs, Colorado Springs, CO 80918, USA.
FAU - Spendier, Kathrin
AU  - Spendier K
AUID- ORCID: 0000-0002-5784-5253
AD  - UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado 
      Springs, Colorado Springs, CO 80918, USA.
AD  - Department of Physics and Energy Science, University of Colorado at Colorado 
      Springs, Colorado Springs, CO 80918, USA.
FAU - Hagen, Guy M
AU  - Hagen GM
AD  - UCCS Center for the Biofrontiers Institute, University of Colorado at Colorado 
      Springs, Colorado Springs, CO 80918, USA.
LA  - eng
GR  - R15 GM128166/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20190704
PL  - Switzerland
TA  - J Imaging
JT  - Journal of imaging
JID - 101698819
PMC - PMC6663088
MID - NIHMS1041488
OTO - NOTNLM
OT  - FcepsilonRI
OT  - IgE receptor
OT  - P-TIRF
OT  - RBL-2H3
OT  - plasma membrane
OT  - rat basophilic leukemia cells
OT  - supported lipid bilayer
OT  - total internal reflection fluorescence microscopy
COIS- Conflicts of Interest: The authors declare no conflict of interest. The funders 
      had no role in the design of the study; in the collection, analyses, or 
      interpretation of data; in the writing of the manuscript, or in the decision to 
      publish the results. Any opinions, findings, and conclusions or recommendations 
      expressed in this material are those of the authors and do not necessarily 
      reflect the views of the National Science Foundation.
EDAT- 2019/07/31 06:00
MHDA- 2019/07/31 06:01
PMCR- 2019/07/04
CRDT- 2019/07/31 06:00
PHST- 2019/07/31 06:00 [entrez]
PHST- 2019/07/31 06:00 [pubmed]
PHST- 2019/07/31 06:01 [medline]
PHST- 2019/07/04 00:00 [pmc-release]
AID - jimaging5070063 [pii]
AID - jimaging-05-00063 [pii]
AID - 10.3390/jimaging5070063 [doi]
PST - ppublish
SO  - J Imaging. 2019 Jul;5(7):63. doi: 10.3390/jimaging5070063. Epub 2019 Jul 4.