PMID- 31361553
OWN - NLM
STAT- MEDLINE
DCOM- 20200320
LR  - 20200320
IS  - 1931-8448 (Electronic)
IS  - 1076-6294 (Linking)
VI  - 25
IP  - 9
DP  - 2019 Nov
TI  - High Prevalence of the aac(6')-Ie-aph(2'')-Ia Gene in Hospital Isolates of 
      Enterococcus faecalis Co-Resistant to Gentamicin and Penicillin.
PG  - 1275-1281
LID - 10.1089/mdr.2018.0466 [doi]
AB  - Objectives: This study aimed to characterize the molecular mechanism of 
      resistance to gentamicin among penicillin-resistant, ampicillin-susceptible 
      Enterococcus faecalis (PRASEF) isolates by investigating the presence of the 
      aac(6')-Ie-aph(2'')-Ia gene. The co-resistance to antimicrobials of other classes 
      was also evaluated. Results: Among the 151 isolates evaluated, 70 were PRASEF and 
      81 were penicillin-susceptible and ampicillin-susceptible E. faecalis (PSASEF). 
      No beta-lactamase producing isolate was detected. Eighty-three (55.0%) and 35 
      (23.2%) out of the 151 E. faecalis isolates showed high-level gentamicin 
      resistance (HLGR) and high-level streptomycin resistance (HLSR) phenotypes. 
      However, a significantly higher rate of PRASEF (88.6%) showed HLGR phenotype in 
      comparison with PSASEF (23.5%) (p < 0.01). Conversely, a significantly lower rate 
      of PRASEF (14.3%) showing HLSR was observed in comparison with PSASEF (30.9%) 
      (p = 0.02). The prevalence of isolates displaying multidrug resistance (MDR) 
      phenotype was significantly higher (p < 0.01) in the group of PRASEF (81.4%) than 
      in PSASEF (18.6%). The majority of PSASEF (61.9%) and PRASEF (90.3%) isolates 
      showing HLGR phenotype was harboring the aac(6')-Ie-aph(2'')-Ia gene, which 
      encodes a bifunctional enzyme that inactivates all aminoglycosides except 
      streptomycin. Conclusion: The aac(6')-Ie-aph(2'')-Ia gene was prevalent among the 
      Brazilian PRASEF isolates that usually exhibit co-resistance to gentamicin and to 
      multiple other drugs.
FAU - Costa, Luciano Bernardino da
AU  - Costa LBD
AD  - Instituto de Ciencias Biologicas e Naturais, Universidade Federal do Triangulo 
      Mineiro, Uberaba, Brazil.
FAU - Cora, Luiza Franco
AU  - Cora LF
AD  - Instituto de Ciencias Biologicas e Naturais, Universidade Federal do Triangulo 
      Mineiro, Uberaba, Brazil.
FAU - Correa, Fabio Ederson Lopes
AU  - Correa FEL
AD  - Instituto de Ciencias Biologicas e Naturais, Universidade Federal do Triangulo 
      Mineiro, Uberaba, Brazil.
FAU - Gabrielli, Lucas Cabrini
AU  - Gabrielli LC
AD  - Instituto de Ciencias Biologicas e Naturais, Universidade Federal do Triangulo 
      Mineiro, Uberaba, Brazil.
FAU - de Oliveira, Matheus Ribeiro
AU  - de Oliveira MR
AD  - Instituto de Ciencias Biologicas e Naturais, Universidade Federal do Triangulo 
      Mineiro, Uberaba, Brazil.
FAU - Conceicao, Natalia
AU  - Conceicao N
AD  - Instituto de Ciencias Biologicas e Naturais, Universidade Federal do Triangulo 
      Mineiro, Uberaba, Brazil.
AD  - Instituto Federal de Educacao, Ciencia e Tecnologia de Rondonia, Colorado do 
      Oeste, Brazil.
FAU - Oliveira, Adriana Goncalves
AU  - Oliveira AG
AD  - Instituto de Ciencias Biologicas e Naturais, Universidade Federal do Triangulo 
      Mineiro, Uberaba, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20190730
PL  - United States
TA  - Microb Drug Resist
JT  - Microbial drug resistance (Larchmont, N.Y.)
JID - 9508567
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Gentamicins)
RN  - 0 (Penicillins)
RN  - EC 2.- (6'-aminoglycoside acetyltransferase-2''-aminoglycoside 
      phosphotransferase)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
SB  - IM
MH  - Acetyltransferases/genetics
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Brazil
MH  - Child
MH  - Child, Preschool
MH  - Drug Resistance, Multiple, Bacterial
MH  - Enterococcus faecalis/*drug effects/genetics/isolation & purification
MH  - Female
MH  - Gentamicins/administration & dosage/*pharmacology
MH  - Gram-Positive Bacterial Infections/*drug therapy/microbiology
MH  - Hospitals
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Penicillins/administration & dosage/*pharmacology
MH  - Phosphotransferases (Alcohol Group Acceptor)/genetics
MH  - Prevalence
MH  - Young Adult
OTO - NOTNLM
OT  - aminoglycoside-modifying enzyme
OT  - gentamicin resistance
OT  - high-level aminoglycoside resistance
OT  - multidrug-resistant enterococci
OT  - penicillin resistance
EDAT- 2019/07/31 06:00
MHDA- 2020/03/21 06:00
CRDT- 2019/07/31 06:00
PHST- 2019/07/31 06:00 [pubmed]
PHST- 2020/03/21 06:00 [medline]
PHST- 2019/07/31 06:00 [entrez]
AID - 10.1089/mdr.2018.0466 [doi]
PST - ppublish
SO  - Microb Drug Resist. 2019 Nov;25(9):1275-1281. doi: 10.1089/mdr.2018.0466. Epub 
      2019 Jul 30.