PMID- 31364020
OWN - NLM
STAT- MEDLINE
DCOM- 20200309
LR  - 20220125
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 5
DP  - 2019 Oct
TI  - Seleno-beta-lactoglobulin (Se-beta-Lg) induces mitochondria-dependant apoptosis in 
      HepG2 cells.
PG  - 5025-5031
LID - 10.1007/s11033-019-04953-x [doi]
AB  - Selenium compounds have been widely investigated as novel anticancer agents due 
      to high efficacy and selectivity against cancer cells in recent years. This study 
      aimed to research the potential inhibitory effects of seleno-beta-lactoglobulin 
      (Se-beta-Lg) on HepG2 cells in vitro. MTT results demonstrated that the synthetized 
      Se-beta-Lg exhibited strong antitumor activity on HepG2 cells with few side effects 
      on human normal cells (LO2) and relatively weaker cytotoxic effects compared to 
      inorganic selenium (SeO(2)). Scanning electron microscope (SEM), hoechst 33342/PI 
      double staining, annexin V-FITC/PI staining and cell cycle detection results 
      showed that Se-beta-Lg could induce the apoptosis of HepG2 cells via arresting them 
      in S and G2/M phases and lead to the obvious morphological changes (loss of 
      adhesion, cell shrinkage, and membrane blebbing, membrane permeabilities and DNA 
      fragmentation). Besides, JC-1 staining, western blotting (WB) and polymerase 
      chain reaction (PCR) results showed that Se-beta-Lg could gradually destroy the 
      mitochondrial membrane potential of HepG2 cells, and finally resulting in the 
      mitochondria-dependant apoptosis via up-regulation of Bax, Cytochrome c, 
      Caspase-3 and down-regulation of Bcl-2. Our data could provide a theoretical 
      basis for practical application of Se-beta-Lg in food and drug industries.
FAU - Ji, Hai-Yu
AU  - Ji HY
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science and Technology, 
      No. 29, 13th Street, TEDA, Tianjin, 300457, People's Republic of China.
AD  - QingYunTang Biotech (Beijing) Co., Ltd., Beijing, 100176, China.
FAU - Yu, Juan
AU  - Yu J
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science and Technology, 
      No. 29, 13th Street, TEDA, Tianjin, 300457, People's Republic of China.
AD  - QingYunTang Biotech (Beijing) Co., Ltd., Beijing, 100176, China.
FAU - Liu, An-Jun
AU  - Liu AJ
AUID- ORCID: 0000-0003-1820-7299
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science and Technology, 
      No. 29, 13th Street, TEDA, Tianjin, 300457, People's Republic of China. 
      laj@tust.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190730
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Lactoglobulins)
RN  - 0 (Organoselenium Compounds)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Cycle/drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Hep G2 Cells
MH  - Humans
MH  - Lactoglobulins/*pharmacology
MH  - Liver Neoplasms/*drug therapy/pathology
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mitochondria/metabolism
MH  - Organoselenium Compounds/*pharmacology
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Cell apoptosis
OT  - Mitochondria pathway
OT  - Seleno-beta-lactoglobulin (Se-beta-Lg)
EDAT- 2019/08/01 06:00
MHDA- 2020/03/10 06:00
CRDT- 2019/08/01 06:00
PHST- 2019/05/12 00:00 [received]
PHST- 2019/06/27 00:00 [accepted]
PHST- 2019/08/01 06:00 [pubmed]
PHST- 2020/03/10 06:00 [medline]
PHST- 2019/08/01 06:00 [entrez]
AID - 10.1007/s11033-019-04953-x [pii]
AID - 10.1007/s11033-019-04953-x [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Oct;46(5):5025-5031. doi: 10.1007/s11033-019-04953-x. Epub 
      2019 Jul 30.