PMID- 31364099
OWN - NLM
STAT- MEDLINE
DCOM- 20210128
LR  - 20210128
IS  - 1735-546X (Electronic)
IS  - 1735-1308 (Linking)
VI  - 17
IP  - 3
DP  - 2020 May 16
TI  - Protective Effects of Colchicine on Testicular Torsion/Detorsion-Induced 
      Ischemia/Reperfusion Injury in Rats.
PG  - 294-300
LID - 10.22037/uj.v0i0.4918 [doi]
AB  - PURPOSE: To evaluate the short-term use of colchicine on preventing 
      ischemia-reperfusion injury after surgery in an experimental animal model. 
      MATERIALS AND METHODS: A total of 40 rats were divided into five groups (n = 8). 
      Sham (Sh), ischemia-reperfusion (I/R), I/R and colchicine-treated for once 
      per-operatively (I/Rc1), I/R and colchicine-treated for 5 days postoperatively 
      (I/Rc5), and I/R and placebo given for 5 days (I/Rp) groups. Testicular torsion 
      was created by rotating the testicle 720o in clockwise direction and held for 3 
      hours. In group I/Rc1 30 minutes before detorsion, p.o. 1 mg/kg mL infusion of 
      colchicine was given only once. In group I/Rc5, colchicine continued p.o. once 
      daily for five days. Tissue malonyldialdehite (MDA), superoxide dismutase (SOD), 
      glutathione peroxidase (GPx) and catalase (CAT) were measured for evaluating the 
      oxidative stress. Apoptosis levels shown with Caspase-3 staining and mean 
      seminiferous tubular diameter (MSTD), germinal epithelial cell thickness (GECT), 
      and mean testicular biopsy score (MTBS) were used to evaluate the germ cell 
      damage. RESULTS: Decreased protein MDA levels therewithal increased SOD, CAT and 
      GPx levels achieved in I/Rc5 group when compared to I/R group and did not differ 
      from the I/Rp group (p<0.05). MSTD, GECT, and JS were better in I/Rc5 than I/Rp 
      which showed the natural course of I/R damage in testis (p<0.005). Caspase 3 
      positivity, as an apoptosis indicator, were significantly lower (p<0.05) in I/Rc5 
      group in comparison with I/R, I/Rc1, and I/Rp groups. CONCLUSION: The usage of 
      colchicine as a complementary treatment after definitive surgery reduce 
      early-onset ischemia-reperfusion damage and diminishes apoptosis.
FAU - Gozukara, Kerem Han
AU  - Gozukara KH
AD  - Urology Department , Mustafa Kemal University School of Medicine, Turkey. 
      keremgozukara@gmail.com.
FAU - Ozcan, Oguzhan
AU  - Ozcan O
AD  - Biochemistry Department , Mustafa Kemal University School of Medicine, Turkey.
FAU - Ozgur, Tumay
AU  - Ozgur T
AD  - Pathology Department, Mustafa Kemal University School of Medicine, Turkey.
FAU - Kaya, Yusuf Selim
AU  - Kaya YS
AD  - Urology Department , Mustafa Kemal University School of Medicine, Turkey.
FAU - Tutuk, Okan
AU  - Tutuk O
AD  - Physiology Department, Mustafa Kemal University School of Medicine, Turkey.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20200516
PL  - Iran
TA  - Urol J
JT  - Urology journal
JID - 101286676
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Animals
MH  - Colchicine/*therapeutic use
MH  - Male
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*etiology/*prevention & control
MH  - Spermatic Cord Torsion/*complications
MH  - Testis/*blood supply
EDAT- 2019/08/01 06:00
MHDA- 2021/01/29 06:00
CRDT- 2019/08/01 06:00
PHST- 2018/10/29 00:00 [received]
PHST- 2019/06/20 00:00 [accepted]
PHST- 2019/05/26 00:00 [revised]
PHST- 2019/08/01 06:00 [pubmed]
PHST- 2021/01/29 06:00 [medline]
PHST- 2019/08/01 06:00 [entrez]
AID - 4918 [pii]
AID - 10.22037/uj.v0i0.4918 [doi]
PST - epublish
SO  - Urol J. 2020 May 16;17(3):294-300. doi: 10.22037/uj.v0i0.4918.