PMID- 31367490
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220409
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 7
DP  - 2019
TI  - Construction and integrated analysis of crosstalking ceRNAs networks in laryngeal 
      squamous cell carcinoma.
PG  - e7380
LID - 10.7717/peerj.7380 [doi]
LID - e7380
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is one of the most common 
      malignant tumours of the head and neck. Recent evidence has demonstrated that 
      lncRNAs play important roles in tumour progression and could be used as 
      biomarkers for early diagnosis, prognosis, and potential therapeutic targets. The 
      "competitive endogenous RNA (ceRNA)" hypothesis states that lncRNAs competitively 
      bind to miRNAs through their intramolecular miRNA reaction elements (MREs) to 
      construct a wide range of ceRNA regulatory networks. This study aims to predict 
      the role of ceRNA network in LSCC, for advancing the understanding of underlying 
      mechanisms of tumorigenesis. MATERIAL AND METHODS: In this study, the functions 
      of lncRNAs as ceRNAs in LSCC and their prognostic significance were investigated 
      via comprehensive integrated expression profiles data of lncRNAs, mRNAs, and 
      miRNAs obtained from The Cancer Genome Atlas (TCGA). Protein-protein interaction, 
      gene ontology, pathway, and Kaplan-Meier curves analysis were used to profile the 
      expression and function of altered RNAs in LSCC. RESULTS: As a result, 889 
      lncRNAs, 55 miRNAs and 1946 mRNAs were found to be differentially expressed in 
      LSCC. These altered mRNAs were mainly involved in extracellular matrix 
      organization, calcium signaling, and metabolic pathways. To study the regulatory 
      function of lncRNAs, an lncRNA-mediated ceRNA network was constructed. This ceRNA 
      network included 61 lncRNAs, seven miRNAs and seven target mRNAs. Of these RNAs, 
      lncRNAs (TSPEAR-AS, CASK-AS1, MIR137HG, PART1, LSAMP-AS1), miRNA (has-mir-210) 
      and mRNAs (HOXC13, STC2, DIO1, FOXD4L1) had a significant effect on the prognosis 
      of LSCC. CONCLUSION: The results of this study broaden the understanding of the 
      mechanisms by which lncRNAs are involved in tumorigenesis. Furthermore, five 
      lncRNAs (TSPEAR-AS, CASK-AS1, MIR137HG, PART1, LSAMP-AS1) were identified as 
      potential prognostic biomarkers and therapeutic targets for LSCC. These results 
      provide a basis for further experimental and clinical research.
FAU - Liu, Yuehui
AU  - Liu Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi Province, People's Republic of 
      China.
FAU - Ye, Fan
AU  - Ye F
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi Province, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
DEP - 20190722
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC6657684
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - The cancer genome atlas
OT  - ceRNA
OT  - lncRNA
COIS- The authors declare there are no competing interests.
EDAT- 2019/08/02 06:00
MHDA- 2019/08/02 06:01
PMCR- 2019/07/22
CRDT- 2019/08/02 06:00
PHST- 2019/05/06 00:00 [received]
PHST- 2019/06/29 00:00 [accepted]
PHST- 2019/08/02 06:00 [entrez]
PHST- 2019/08/02 06:00 [pubmed]
PHST- 2019/08/02 06:01 [medline]
PHST- 2019/07/22 00:00 [pmc-release]
AID - 7380 [pii]
AID - 10.7717/peerj.7380 [doi]
PST - epublish
SO  - PeerJ. 2019 Jul 22;7:e7380. doi: 10.7717/peerj.7380. eCollection 2019.