PMID- 31369718
OWN - NLM
STAT- MEDLINE
DCOM- 20200923
LR  - 20231213
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 415
DP  - 2019 Sep 1
TI  - Over-Expression of TRPC6 via CRISPR Based Synergistic Activation Mediator in 
      BMSCs Ameliorates Brain Injury in a Rat Model of Cerebral Ischemia/Reperfusion.
PG  - 147-160
LID - S0306-4522(19)30468-3 [pii]
LID - 10.1016/j.neuroscience.2019.06.041 [doi]
AB  - Stroke is a major life-threatening and disabling disease with a restricted 
      therapeutic approach. Bone marrow stromal cells (BMSCs) possess proliferative 
      ability and a multi-directional differentiation potential, and secrete a range of 
      trophic/growth factors that can protect neurons after cerebral 
      ischemia/reperfusion. Transient receptor potential canonical (TRPC) is a family 
      of non-selective channels permeable to Ca(2+), with several functions including 
      neuronal survival. Over-expression of TRPC6, a subtype of the TRPC family, was 
      shown to protect neurons against cerebral ischemia/reperfusion injury. However, 
      it remains unclear whether over-expression of TRPC6 in BMSCs can further reduce 
      brain injury after ischemia/reperfusion. In the present study, we report that 
      over-expression of TRPC6 via a CRISPR-based synergistic activation mediator in 
      BMSCs provided a greater reduction of brain injury in a rat model of 
      ischemia/reperfusion. Further, the improved neurofunctional outcomes were 
      associated with increased TRPC6 and brain derived neurotrophic factor expression 
      levels. Overall, these data suggest that TRPC6 over-expressing BMSCs may be a 
      promising therapeutic agent for ischemic stroke.
CI  - Copyright (c) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Li, Wenbin
AU  - Li W
AD  - Department of Neurology, First Clinical College of Harbin Medical University, 
      Room 501, Building 3, 23 Youzheng Street, Harbin, Heilongjiang Province, 150001, 
      People's Republic of China.
FAU - Yang, Fan
AU  - Yang F
AD  - Department of Neurology, First Clinical College of Harbin Medical University, 
      Room 501, Building 3, 23 Youzheng Street, Harbin, Heilongjiang Province, 150001, 
      People's Republic of China.
FAU - Gao, Jinxing
AU  - Gao J
AD  - Department of Neurology, First Clinical College of Harbin Medical University, 
      Room 501, Building 3, 23 Youzheng Street, Harbin, Heilongjiang Province, 150001, 
      People's Republic of China.
FAU - Tang, Yushi
AU  - Tang Y
AD  - Department of Neurology, First Clinical College of Harbin Medical University, 
      Room 501, Building 3, 23 Youzheng Street, Harbin, Heilongjiang Province, 150001, 
      People's Republic of China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Neurology, First Clinical College of Harbin Medical University, 
      Room 501, Building 3, 23 Youzheng Street, Harbin, Heilongjiang Province, 150001, 
      People's Republic of China.
FAU - Pan, Yujun
AU  - Pan Y
AD  - Department of Neurology, First Clinical College of Harbin Medical University, 
      Room 501, Building 3, 23 Youzheng Street, Harbin, Heilongjiang Province, 150001, 
      People's Republic of China. Electronic address: yujunpan@ems.hrbmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190729
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Creb1 protein, rat)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (Trpc6 protein, rat)
RN  - 12634-43-4 (Spectrin)
RN  - EC 3.4.22.- (Calpain)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Calpain/metabolism
MH  - Cell Survival
MH  - Clustered Regularly Interspaced Short Palindromic Repeats
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells
MH  - Models, Animal
MH  - Neurons/metabolism
MH  - Neuroprotection/*genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*metabolism
MH  - Spectrin/metabolism
MH  - TRPC Cation Channels/genetics/*metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - BDNF
OT  - BMSCs
OT  - CRISPR
OT  - TRPC6
OT  - ischemia/reperfusion
EDAT- 2019/08/02 06:00
MHDA- 2020/09/24 06:00
CRDT- 2019/08/02 06:00
PHST- 2018/12/15 00:00 [received]
PHST- 2019/06/26 00:00 [revised]
PHST- 2019/06/27 00:00 [accepted]
PHST- 2019/08/02 06:00 [pubmed]
PHST- 2020/09/24 06:00 [medline]
PHST- 2019/08/02 06:00 [entrez]
AID - S0306-4522(19)30468-3 [pii]
AID - 10.1016/j.neuroscience.2019.06.041 [doi]
PST - ppublish
SO  - Neuroscience. 2019 Sep 1;415:147-160. doi: 10.1016/j.neuroscience.2019.06.041. 
      Epub 2019 Jul 29.