PMID- 31370145
OWN - NLM
STAT- MEDLINE
DCOM- 20191231
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 15
DP  - 2019 Jul 31
TI  - Silencing of the Chitin Synthase Gene Is Lethal to the Asian Citrus Psyllid, 
      Diaphorina citri.
LID - 10.3390/ijms20153734 [doi]
LID - 3734
AB  - Chitin synthase is a critical enzyme that catalyzes N-acetylglucosamine to form 
      chitin, which plays an important role in the growth and development of insects. 
      In this study, we identified a chitin synthase gene (CHS) with a complete open 
      reading frame (ORF) of 3180 bp from the genome database of Diaphorina citri, 
      encoding a protein of 1059 amino acid residues with the appropriate signature 
      motifs (EDR and QRRRW). Reverse transcription-quantitative PCR (RT-qPCR) analysis 
      suggested that D. citri CHS (DcCHS) was expressed throughout all developmental 
      stages and all tissues. DcCHS had the highest expression level in the integument 
      and fifth-instar nymph stage. Furthermore, the effects of diflubenzuron (DFB) on 
      D. citri mortality and DcCHS expression level were investigated using 
      fifth-instar nymph through leaf dip bioassay, and the results revealed that the 
      nymph exposed to DFB had the highest mortality compared with control group 
      (Triton-100). Silencing of DcCHS by RNA interference resulted in malformed 
      phenotypes and increased mortality with decreased molting rate. In addition, 
      transmission electron microscopy (TEM) and fluorescence in situ hybridization 
      (FISH) also revealed corresponding ultrastructural defects. Our results suggest 
      that DcCHS might play an important role in the development of D. citri and can be 
      used as a potential target for psyllid control.
FAU - Lu, Zhan-Jun
AU  - Lu ZJ
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Huang, Yu-Ling
AU  - Huang YL
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Yu, Hai-Zhong
AU  - Yu HZ
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China. 
      yuhaizhong1988@163.com.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China. 
      yuhaizhong1988@163.com.
FAU - Li, Ning-Yan
AU  - Li NY
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Xie, Yan-Xin
AU  - Xie YX
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Zhang, Qin
AU  - Zhang Q
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Zeng, Xiang-Dong
AU  - Zeng XD
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Hu, Hao
AU  - Hu H
AD  - Saskatoon Research Center, Agriculture and Agri-Food Canada, Saskatoon, SK S7N 
      0X2, Canada.
FAU - Huang, Ai-Jun
AU  - Huang AJ
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Yi, Long
AU  - Yi L
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
FAU - Su, Hua-Nan
AU  - Su HN
AD  - College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
AD  - National Navel Orange Engineering Research Center, Ganzhou 341000, China.
LA  - eng
GR  - 31560602/National Natural Science Foundation of China/
GR  - 2018YFD0201504/Ministry of Science and Technology of China/
GR  - GJJ180747/Educational Commission of Jiangxi Province of China/
GR  - 20171BCB23074/Science and Technology Department of Jiangxi Province/
PT  - Journal Article
DEP - 20190731
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Insect Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.4.1.16 (Chitin Synthase)
RN  - J76U6ZSI8D (Diflubenzuron)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chitin Synthase/antagonists & inhibitors/*genetics/metabolism
MH  - Citrus/parasitology
MH  - Diflubenzuron/pharmacology
MH  - Fruit/parasitology
MH  - Gene Expression Regulation, Developmental
MH  - *Genome, Insect
MH  - Hemiptera/drug effects/enzymology/*genetics/growth & development
MH  - Insect Control
MH  - Insect Proteins/antagonists & inhibitors/*genetics/metabolism
MH  - Molting/drug effects/genetics
MH  - Nymph/drug effects/*genetics/growth & development/metabolism
MH  - Open Reading Frames
MH  - Phylogeny
MH  - *RNA Interference
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
PMC - PMC6696430
OTO - NOTNLM
OT  - Diaphorina citri
OT  - RNA interference
OT  - chitin synthase
OT  - diflubenzuron
COIS- The authors declare no conflict of interest.
EDAT- 2019/08/03 06:00
MHDA- 2020/01/01 06:00
PMCR- 2019/08/01
CRDT- 2019/08/03 06:00
PHST- 2019/06/29 00:00 [received]
PHST- 2019/07/27 00:00 [revised]
PHST- 2019/07/29 00:00 [accepted]
PHST- 2019/08/03 06:00 [entrez]
PHST- 2019/08/03 06:00 [pubmed]
PHST- 2020/01/01 06:00 [medline]
PHST- 2019/08/01 00:00 [pmc-release]
AID - ijms20153734 [pii]
AID - ijms-20-03734 [pii]
AID - 10.3390/ijms20153734 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Jul 31;20(15):3734. doi: 10.3390/ijms20153734.