PMID- 31370803
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20231013
IS  - 1528-3658 (Electronic)
IS  - 1076-1551 (Print)
IS  - 1076-1551 (Linking)
VI  - 25
IP  - 1
DP  - 2019 Aug 1
TI  - Gut microbiota promote the inflammatory response in the pathogenesis of systemic 
      lupus erythematosus.
PG  - 35
LID - 10.1186/s10020-019-0102-5 [doi]
LID - 35
AB  - OBJECTIVES: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease 
      whose onset and progression are affected by genetic and environmental factors. 
      The purpose of this study is to identify the influence of gut microbiota in the 
      pathogenesis of SLE, and to investigate the mechanism involved. METHODS: Fecal 
      microbiota from C57/BL6 mice and SLE prone mice were examined using 
      next-generation sequencing (NGS). Germ free mice were given fecal microbiota 
      transplantation (FMT), and their gut microbiome and gene expression in 
      recipients' colons were examined by NGS. The anti-double stranded DNA 
      (anti-dsDNA) antibodies in recipients were determined using an enzyme-linked 
      immunosorbent assay (ELISA). The immune cell profiles of mice were analyzed by 
      flow cytometry at the 3rd week after FMT, and the expression of genes associated 
      with SLE after FMT was determined using quantitative real-time PCR (qRT-PCR). 
      RESULTS: The fecal microbiota of SLE mice had lower community richness and 
      diversity than healthy mice. Fecal microbiota of recipient mice were similar to 
      their donors. Fecal microbiome from SLE mice could lead to a significant increase 
      of anti-dsDNA antibodies and promote the immune response in recipient mice. Our 
      results also indicated that fecal microbiome from SLE mice resulted in 
      significant changes in the distribution of immune cells and upregulated 
      expression of certain lupus susceptibility genes. CONCLUSIONS: SLE is associated 
      with alterations of gut microbiota. Fecal microbiome from SLE mice can induce the 
      production of anti-dsDNA antibodies in germ free mice and stimulate the 
      inflammatory response, and alter the expression of SLE susceptibility genes in 
      these mice.
FAU - Ma, Yiyangzi
AU  - Ma Y
AD  - NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of 
      Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union 
      Medical College; Key Laboratory of Human Diseases Animal Model, State 
      Administration of Traditional Chinese Medicine, Beijing, China.
FAU - Xu, Xiaoxue
AU  - Xu X
AD  - Department of Core Facility Center, Capital Medical University, Beijing, China.
FAU - Li, Mengtao
AU  - Li M
AD  - Division of Rheumatology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences & Peking Union Medical College, Beijing, China.
FAU - Cai, Jun
AU  - Cai J
AD  - Hypertension Center, Fuwai hospital, State Key Laboratory of Cardiovascular 
      Disease of China, National Center for Cardiovascular Disease of China, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Beijing, China. 
      caijun7879@126.com.
FAU - Wei, Qiang
AU  - Wei Q
AD  - NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of 
      Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union 
      Medical College; Key Laboratory of Human Diseases Animal Model, State 
      Administration of Traditional Chinese Medicine, Beijing, China. 
      weiqiang0430@cnilas.org.
FAU - Niu, Haitao
AU  - Niu H
AUID- ORCID: 0000-0003-2210-2051
AD  - NHC Key Laboratory of Human Disease Comparative Medicine, The Institute of 
      Laboratory Animal Sciences, Chinese Academy of Medical Sciences & Peking Union 
      Medical College; Key Laboratory of Human Diseases Animal Model, State 
      Administration of Traditional Chinese Medicine, Beijing, China. 
      niuhaitao@cnilas.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190801
PL  - England
TA  - Mol Med
JT  - Molecular medicine (Cambridge, Mass.)
JID - 9501023
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fecal Microbiota Transplantation
MH  - Feces/*microbiology
MH  - Female
MH  - Gastrointestinal Microbiome/*physiology
MH  - High-Throughput Nucleotide Sequencing
MH  - Inflammation/*microbiology
MH  - Lupus Erythematosus, Systemic/*immunology/*microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microbiota/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC6676588
OTO - NOTNLM
OT  - Fecal microbiota transplantation2
OT  - Gut microbiota1
OT  - Immune response4
OT  - Lupus susceptibility gene5
OT  - Systemic lupus erythematosus3
COIS- The authors declare that they have no competing interests.
EDAT- 2019/08/03 06:00
MHDA- 2020/06/23 06:00
PMCR- 2019/08/01
CRDT- 2019/08/03 06:00
PHST- 2019/04/06 00:00 [received]
PHST- 2019/06/25 00:00 [accepted]
PHST- 2019/08/03 06:00 [entrez]
PHST- 2019/08/03 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2019/08/01 00:00 [pmc-release]
AID - 10.1186/s10020-019-0102-5 [pii]
AID - 102 [pii]
AID - 10.1186/s10020-019-0102-5 [doi]
PST - epublish
SO  - Mol Med. 2019 Aug 1;25(1):35. doi: 10.1186/s10020-019-0102-5.