PMID- 31372553
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 5
IP  - 7
DP  - 2019 Jul
TI  - Influence of environment on the pathogenesis of Ostreid herpesvirus-1 (OsHV-1) 
      infections in Pacific oysters (Crassostrea gigas) through differential microbiome 
      responses.
PG  - e02101
LID - 10.1016/j.heliyon.2019.e02101 [doi]
LID - e02101
AB  - The oyster microbiome is thought to contribute to the pathogenesis of mass 
      mortality disease in Pacific oysters, associated with OsHV-1. As filter-feeders, 
      oysters host a microbiota that can be influenced by the estuarine environment. 
      This may alter susceptibility to OsHV-1 infections, causing variable mortality. 
      This study aimed at: (1) differences in the microbiome of Pacific oysters with a 
      common origin but grown in geographically distinct estuaries; (2) evaluating 
      changes occurring in the microbiota, especially in Vibrio, and (3) differential 
      responses of the oyster microbiome, in response to an OsHV-1 infection. Pacific 
      oysters sourced from a single hatchery but raised separately in Patonga Creek, 
      Shoalhaven River and Clyde River of NSW, Australia, were used and challenged with 
      OsHV-1. The initial microbiome composition was different in the three batches and 
      changed further, post-injection (p < 0.05). The Patonga oysters with the highest 
      mortality also had higher OsHV-1 and Vibrio quantities compared to the other two 
      batches (p < 0.05). The higher initial bacterial diversity in Patonga oysters 
      decreased in moribund oysters which was not observed in the other two batches (p 
      < 0.05). The microbiome of survivors of OsHV-1 infection and negative control 
      oysters of two batches, did not show any changes with the relevant pre-challenged 
      microbiome. A strong correlation was observed between the OsHV-1 and Vibrio 
      quantities in OsHV-1 infected oysters (r = 0.6; p < 0.001). In conclusion, the 
      Pacific oyster microbiome differed in different batches despite a common hatchery 
      origin. Different microbiomes responded differently with a differential outcome 
      of OsHV-1 challenge. The higher Vibrio load in oysters with higher OsHV-1 content 
      and higher mortality, suggests a role in Vibrio in the pathogenesis of this 
      mortality disease. This study provided insights of the potential of different 
      estuarine environments to shape the Pacific oyster microbiome and how different 
      microbiomes are associated with different outcomes of OsHV-1 infection.
FAU - Pathirana, Erandi
AU  - Pathirana E
AD  - Sydney School of Veterinary Science, Faculty of Science, University of Sydney, 
      Camden, NSW, 2570, Australia.
FAU - Fuhrmann, Marine
AU  - Fuhrmann M
AD  - Sydney School of Veterinary Science, Faculty of Science, University of Sydney, 
      Camden, NSW, 2570, Australia.
FAU - Whittington, Richard
AU  - Whittington R
AD  - Sydney School of Veterinary Science, Faculty of Science, University of Sydney, 
      Camden, NSW, 2570, Australia.
FAU - Hick, Paul
AU  - Hick P
AD  - Sydney School of Veterinary Science, Faculty of Science, University of Sydney, 
      Camden, NSW, 2570, Australia.
LA  - eng
PT  - Journal Article
DEP - 20190720
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC6656993
OTO - NOTNLM
OT  - Bioinformatics
OT  - Microbiology
OT  - Molecular biology
OT  - Virology
EDAT- 2019/08/03 06:00
MHDA- 2019/08/03 06:01
PMCR- 2019/07/20
CRDT- 2019/08/03 06:00
PHST- 2019/03/12 00:00 [received]
PHST- 2019/04/26 00:00 [revised]
PHST- 2019/07/15 00:00 [accepted]
PHST- 2019/08/03 06:00 [entrez]
PHST- 2019/08/03 06:00 [pubmed]
PHST- 2019/08/03 06:01 [medline]
PHST- 2019/07/20 00:00 [pmc-release]
AID - S2405-8440(19)35761-5 [pii]
AID - e02101 [pii]
AID - 10.1016/j.heliyon.2019.e02101 [doi]
PST - epublish
SO  - Heliyon. 2019 Jul 20;5(7):e02101. doi: 10.1016/j.heliyon.2019.e02101. eCollection 
      2019 Jul.