PMID- 31373105
OWN - NLM
STAT- MEDLINE
DCOM- 20200707
LR  - 20200707
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 86
IP  - 11
DP  - 2019 Nov
TI  - The Cxadr-Adam10 complex plays pivotal roles in tight junction integrity and 
      early trophoblast development in mice.
PG  - 1628-1638
LID - 10.1002/mrd.23250 [doi]
AB  - Understanding preimplantation embryo development has important implications for 
      assisted reproductive technologies (ARTs) after the introduction of in vitro 
      fertilisation and embryo transfer because most embryonic losses occur during 
      pre/peri-implantation. Recent studies have shown that tight junctions (TJs) are 
      important components for embryos to develop to the blastocyst stage. However, 
      their biological function after cavitation has not been extensively studied. We 
      examined TJ assembly focusing on coxsackievirus and adenovirus receptor (Cxadr) 
      and A disintegrin and metalloproteinase 10 (Adam10) using siRNA and/or an 
      Adam10-specific inhibitor (GI254023X). TJ-associated genes, including occludin 
      and tight junction protein 1 (Tjp1), were downregulated in the Cxadr knockdown 
      (KD) embryos but were unaltered in Adam10 KD embryos. However, Adam10 KD or 
      chemical inhibition affected subcellular localisation of Adam10, Cxadr, and Tjp1, 
      leading to disrupted TJ assembly. Furthermore, Cxadr KD or GI254023X-treated 
      blastocysts showed a relatively smaller outgrowth area and aberrant expression of 
      transcription factor AP-2gamma, a trophoblast-specific marker in the in vitro embryo 
      outgrowth assay. In summary, we demonstrated that the Cxadr-Adam10 complex might 
      moderate TJ integrity/stability and play pivotal roles during early embryonic 
      development. Collectively, understanding the establishment of the TJ complex and 
      its integrity will provide insight into translational research for predicting and 
      selecting developmental competency for ART.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Jeong, Yelin
AU  - Jeong Y
AD  - Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, 
      Chungnam National University, Daejeon, Republic of Korea.
AD  - Disease Model Research Laboratory, Genome Editing Research Center, Korea Research 
      Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
FAU - Ock, Sun-A
AU  - Ock SA
AD  - National Institute of Animal Science, Rural Development Administration, 
      Jeollabuk-do, Republic of Korea.
FAU - Yoo, Jae Gyu
AU  - Yoo JG
AD  - National Institute of Animal Science, Rural Development Administration, 
      Jeollabuk-do, Republic of Korea.
FAU - Yu, Dae-Yeul
AU  - Yu DY
AD  - Disease Model Research Laboratory, Genome Editing Research Center, Korea Research 
      Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
AD  - Department of Functional Genomics, University of Science and Technology, Daejeon, 
      Republic of Korea.
FAU - Choi, Inchul
AU  - Choi I
AUID- ORCID: 0000-0001-5011-2658
AD  - Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, 
      Chungnam National University, Daejeon, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190802
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (CLMP protein, mouse)
RN  - 0 (Coxsackie and Adenovirus Receptor-Like Membrane Protein)
RN  - 0 (Membrane Proteins)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tjp1 protein, mouse)
RN  - 0 (Transcription Factor AP-2)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - EC 3.4.- (Amyloid Precursor Protein Secretases)
RN  - EC 3.4.24.81 (ADAM10 Protein)
RN  - EC 3.4.24.81 (Adam10 protein, mouse)
SB  - IM
MH  - ADAM10 Protein/genetics/*metabolism
MH  - Amyloid Precursor Protein Secretases/genetics/*metabolism
MH  - Animals
MH  - Coxsackie and Adenovirus Receptor-Like Membrane Protein/genetics/*metabolism
MH  - Down-Regulation
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Multiprotein Complexes/genetics/*metabolism
MH  - RNA, Small Interfering/genetics
MH  - Tight Junctions/genetics/*metabolism
MH  - Transcription Factor AP-2/genetics/metabolism
MH  - Trophoblasts/cytology/*metabolism
MH  - Zonula Occludens-1 Protein/biosynthesis/genetics
OTO - NOTNLM
OT  - Adam10
OT  - Cxadr
OT  - blastocyst
OT  - embryo transfer
OT  - tight junction
EDAT- 2019/08/03 06:00
MHDA- 2020/07/08 06:00
CRDT- 2019/08/03 06:00
PHST- 2018/12/10 00:00 [received]
PHST- 2019/07/16 00:00 [accepted]
PHST- 2019/08/03 06:00 [pubmed]
PHST- 2020/07/08 06:00 [medline]
PHST- 2019/08/03 06:00 [entrez]
AID - 10.1002/mrd.23250 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2019 Nov;86(11):1628-1638. doi: 10.1002/mrd.23250. Epub 2019 Aug 
      2.