PMID- 31376054
OWN - NLM
STAT- MEDLINE
DCOM- 20200120
LR  - 20200225
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 44
IP  - 9
DP  - 2019 Sep
TI  - NGF, BDNF and Arc mRNA Expression in the Hippocampus of Rats After Administration 
      of Morphine.
PG  - 2139-2146
LID - 10.1007/s11064-019-02851-z [doi]
AB  - Morphine can influence immediate early genes (IEG) of activity-regulated 
      cytoskeletal-associated protein (Arc) and brain-derived neurotrophic factor 
      (BDNF) which are activated in response to physiological stimuli during learning, 
      as well as the nerve growth factor (NGF) gene which increases the expression of 
      several IEGs for memory formation. The purpose of the current study was first to 
      evaluate the effect of acute (1-day) and subchronic (15-days) morphine 
      administration on memory retrieval of rats and second to determine the 
      hippocampal expression of NGF, BDNF and Arc genes as potential contributors in 
      the observed effects in each setting. The effects of morphine (intraperitoneal, 
      10, 15 and 20 mg/kg) on memory function and gene expression were assessed using 
      inhibitory avoidance test and real-time polymerase chain reaction, respectively. 
      We found that a single dose of morphine at the highest dose of 20 mg/kg decreases 
      the post-training step-through-latency, while repeated administration of the same 
      dose for 15 successive days increases this indicator of memory retrieval. We did 
      not detect a significant change in the hippocampal expression of Arc, BDNF or NGF 
      genes after a single episode of morphine treatment. However, subchronic morphine 
      administration (15 and 20 mg/kg) increased the expression of Arc and BDNF genes 
      in a dose dependent manner. A higher mRNA expression for the NGF was observed at 
      the higher dose of 20 mg/kg. We hypothesize that the subchronic effects were 
      morphine-induced behavioral sensitization which may have been enhanced through 
      increased hippocampal Arc expression.
FAU - Rouhani, Fatemeh
AU  - Rouhani F
AD  - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.
FAU - Khodarahmi, Parvin
AU  - Khodarahmi P
AUID- ORCID: 0000-0001-6252-4254
AD  - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran. 
      khodarahmiparvin@yahoo.com.
FAU - Naseh, Vahid
AU  - Naseh V
AD  - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.
LA  - eng
PT  - Journal Article
DEP - 20190802
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (activity regulated cytoskeletal-associated protein)
RN  - 76I7G6D29C (Morphine)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Cytoskeletal Proteins/genetics
MH  - Gene Expression/*drug effects
MH  - Genes, Immediate-Early/drug effects
MH  - Hippocampus/*metabolism
MH  - Learning/*drug effects
MH  - Male
MH  - Memory/*drug effects
MH  - Morphine/*pharmacology
MH  - Nerve Growth Factor/genetics
MH  - Nerve Tissue Proteins/genetics
MH  - RNA, Messenger/*metabolism
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Arc gene
OT  - BDNF gene
OT  - Memory
OT  - Morphine
OT  - NGF gene
OT  - Rat
EDAT- 2019/08/04 06:00
MHDA- 2020/01/21 06:00
CRDT- 2019/08/04 06:00
PHST- 2019/03/27 00:00 [received]
PHST- 2019/07/29 00:00 [accepted]
PHST- 2019/07/26 00:00 [revised]
PHST- 2019/08/04 06:00 [pubmed]
PHST- 2020/01/21 06:00 [medline]
PHST- 2019/08/04 06:00 [entrez]
AID - 10.1007/s11064-019-02851-z [pii]
AID - 10.1007/s11064-019-02851-z [doi]
PST - ppublish
SO  - Neurochem Res. 2019 Sep;44(9):2139-2146. doi: 10.1007/s11064-019-02851-z. Epub 
      2019 Aug 2.