PMID- 31376159
OWN - NLM
STAT- MEDLINE
DCOM- 20200702
LR  - 20221019
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 91
IP  - 2
DP  - 2020 Feb
TI  - Platelet-rich fibrin elicits an anti-inflammatory response in macrophages in 
      vitro.
PG  - 244-252
LID - 10.1002/JPER.19-0216 [doi]
AB  - BACKGROUND: Platelet-rich fibrin (PRF) serves as a reservoir of bioactive 
      molecules to support wound healing and bone regeneration. The beneficial action 
      of PRF might involve macrophage polarization from proinflammatory M1 toward 
      pro-resolving M2 phenotypes. This study aims to evaluate the effect of PRF on 
      macrophage polarization. METHODS: Murine primary macrophages and RAW 264.7 cells 
      were exposed to saliva and lipopolysaccharides (LPS) with and without PRF lysates 
      obtained by repeated freeze-thawing or the secretome of PRF membranes, termed PRF 
      conditioned medium. The expression of the M1 marker genes interleukin 1beta (IL1beta) 
      and interleukin 6 (IL6) along with the M2 markers arginase-1 and chitinase-like 3 
      (Chil3 or YM1) were evaluated by real time polymerase chain reaction. Immunoassay 
      and immunofluorescence staining were performed for IL6 and p65 translocation, a 
      subunit nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), 
      respectively. RESULTS: We report here that PRF lysates and PRF conditioned 
      medium, the latter containing the secretome, greatly decreased the 
      proinflammatory response of primary macrophages and RAW 264.7 cells as indicated 
      by the expression of IL1beta and IL6. The anti-inflammatory activity of PRF lysates 
      was further confirmed by IL6 immunoassay. Moreover, PRF lysates suppressed the 
      translocation of p65 from the cytoplasm into the nucleus after incubation with 
      saliva. In support of M2 polarization, PRF lysates and PRF conditioned medium 
      enhanced the expression of arginase-1 and YM1 in primary macrophages. CONCLUSION: 
      Our results indicate that PRF holds an anti-inflammatory activity and shifts the 
      macrophage polarization from an M1 toward an M2 phenotype.
CI  - (c) 2019 The Authors. Journal of Periodontology published by Wiley Periodicals, 
      Inc. on behalf of American Academy of Periodontology.
FAU - Nasirzade, Jila
AU  - Nasirzade J
AUID- ORCID: 0000-0002-7913-4823
AD  - Department of Oral Biology, Medical University of Vienna, Vienna, Austria.
AD  - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Kargarpour, Zahra
AU  - Kargarpour Z
AUID- ORCID: 0000-0003-3404-3773
AD  - Department of Oral Biology, Medical University of Vienna, Vienna, Austria.
AD  - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Hasannia, Sadegh
AU  - Hasannia S
AD  - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Strauss, Franz Josef
AU  - Strauss FJ
AUID- ORCID: 0000-0002-5832-7327
AD  - Department of Oral Biology, Medical University of Vienna, Vienna, Austria.
AD  - Department of Conservative Dentistry, School of Dentistry, University of Chile, 
      Santiago, Chile.
FAU - Gruber, Reinhard
AU  - Gruber R
AUID- ORCID: 0000-0001-5400-9009
AD  - Department of Oral Biology, Medical University of Vienna, Vienna, Austria.
AD  - Department of Periodontology, School of Dental Medicine, University of Bern, 
      Bern, Switzerland.
LA  - eng
GR  - I 4072/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190914
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents
MH  - Lipopolysaccharides
MH  - Macrophage Activation
MH  - Macrophages
MH  - Mice
MH  - *Platelet-Rich Fibrin
MH  - RAW 264.7 Cells
PMC - PMC7065136
OTO - NOTNLM
OT  - anti-inflammatory agents
OT  - arginase
OT  - blood platelets
OT  - cytokines
OT  - macrophages
OT  - platelet-rich fibrin
EDAT- 2019/08/04 06:00
MHDA- 2020/07/03 06:00
PMCR- 2020/03/11
CRDT- 2019/08/04 06:00
PHST- 2019/04/06 00:00 [received]
PHST- 2019/05/31 00:00 [revised]
PHST- 2019/06/17 00:00 [accepted]
PHST- 2019/08/04 06:00 [pubmed]
PHST- 2020/07/03 06:00 [medline]
PHST- 2019/08/04 06:00 [entrez]
PHST- 2020/03/11 00:00 [pmc-release]
AID - JPER10408 [pii]
AID - 10.1002/JPER.19-0216 [doi]
PST - ppublish
SO  - J Periodontol. 2020 Feb;91(2):244-252. doi: 10.1002/JPER.19-0216. Epub 2019 Sep 
      14.