PMID- 31377002
OWN - NLM
STAT- MEDLINE
DCOM- 20200206
LR  - 20200206
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 121
IP  - 7
DP  - 2019 Oct
TI  - Hyperhomocysteinemia and myocardial remodeling in the sand rat, Psammomys obesus.
PG  - 823-832
LID - S0065-1281(19)30088-1 [pii]
LID - 10.1016/j.acthis.2019.07.008 [doi]
AB  - OBJECTIVE: Numerous studies have shown that a methionine-rich diet induces 
      hyperhomocysteinemia (Hhcy), a risk factor for cardiovascular diseases. The 
      objective of the present study was to determine the involvement of Hhcy in 
      cardiac remodeling in the sand rat Psammomys obesus. MATERIALS AND METHODS: An 
      experimental Hhcy was induced, in the sand rat Psammomys obesus, by 
      intraperitoneal injection of 300 mg/kg of body weight/day of methionine for 1 
      month. The impact of Hhcy on the cellular and matricial structures of the 
      myocardium was analyzed with histological techniques (Masson trichrome and Sirius 
      red staining). Immunohistochemistry allowed us to analyze several factors 
      involved in myocardial remodeling, such as fibrillar collagen I and III, 
      metalloproteases (MMP-2 and -9) and their inhibitors (TIMP-1 and -2), TGF-beta1 and 
      activated caspase 3. RESULTS: Our results show that Hhcy induced by an excess of 
      methionine causes, in the myocardium of Psammomys obesus, a significant 
      accumulation of fibrillar collagens I and III at the interstitial and 
      perivascular scales, indicating the appearance of fibrosis, which is associated 
      with an immuno-expression increase of TGF-beta1, MMP-9 and TIMP-2 and an 
      immuno-expression decrease of MMP-2 and TIMP-1. Also, Hhcy induces apoptosis of 
      some cardiomyocytes and cardiac fibroblasts by increasing of activated caspase 3 
      expression. These results highlight a remodeling of cardiac tissue in 
      hyperhomocysteinemic Psammomys obesus.
CI  - Copyright (c) 2019 Elsevier GmbH. All rights reserved.
FAU - Chaouad, Billel
AU  - Chaouad B
AD  - Biochemistry and Remodeling of the Extracellular Matrix, Laboratory of Cellular 
      and Molecular Biology, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouar, El Alia, 16111, 
      Algiers, Algeria; University Djilali Bounaama of Khemis Miliana, Faculty of 
      Natural and Life Sciences and Earth Sciences, Theniet El Had Road, 44225, Khemis 
      Miliana, Algeria.
FAU - Moudilou, Elara N
AU  - Moudilou EN
AD  - UMRS 449, General Biology - Reproduction and Comparative Development, Lyon 
      Catholic University, UDL, EPHE, PSL, 10, Place des Archives, 69288, Lyon Cedex 
      02, France.
FAU - Ghoul, Adel
AU  - Ghoul A
AD  - Biochemistry and Remodeling of the Extracellular Matrix, Laboratory of Cellular 
      and Molecular Biology, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouar, El Alia, 16111, 
      Algiers, Algeria.
FAU - Zerrouk, Fouzia
AU  - Zerrouk F
AD  - Biochemistry and Remodeling of the Extracellular Matrix, Laboratory of Cellular 
      and Molecular Biology, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouar, El Alia, 16111, 
      Algiers, Algeria.
FAU - Moulahoum, Anissa
AU  - Moulahoum A
AD  - Biochemistry and Remodeling of the Extracellular Matrix, Laboratory of Cellular 
      and Molecular Biology, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouar, El Alia, 16111, 
      Algiers, Algeria.
FAU - Othmani-Mecif, Khira
AU  - Othmani-Mecif K
AD  - Biochemistry and Remodeling of the Extracellular Matrix, Laboratory of Cellular 
      and Molecular Biology, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouar, El Alia, 16111, 
      Algiers, Algeria.
FAU - Cherifi, Mohamed El Hadi
AU  - Cherifi MEH
AD  - Central Laboratory of Biology, EPH Bologhine Ibn Ziri, Algiers, Algeria.
FAU - Exbrayat, Jean-Marie
AU  - Exbrayat JM
AD  - UMRS 449, General Biology - Reproduction and Comparative Development, Lyon 
      Catholic University, UDL, EPHE, PSL, 10, Place des Archives, 69288, Lyon Cedex 
      02, France.
FAU - Benazzoug, Yasmina
AU  - Benazzoug Y
AD  - Biochemistry and Remodeling of the Extracellular Matrix, Laboratory of Cellular 
      and Molecular Biology, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouar, El Alia, 16111, 
      Algiers, Algeria. Electronic address: ybenazzoug@usthb.dz.
LA  - eng
PT  - Journal Article
DEP - 20190731
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Muscle Proteins)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - *Cardiomyopathies/chemically induced/metabolism/pathology
MH  - Gerbillinae
MH  - *Hyperhomocysteinemia/chemically induced/metabolism/pathology
MH  - Methionine/adverse effects/pharmacology
MH  - Muscle Proteins/*biosynthesis
MH  - *Myocardium/metabolism/pathology
MH  - *Myocytes, Cardiac/metabolism/pathology
OTO - NOTNLM
OT  - Apoptosis
OT  - Fibrosis
OT  - Hyperhomocysteinemia
OT  - Myocardium
OT  - Psammomys obesus
EDAT- 2019/08/05 06:00
MHDA- 2020/02/07 06:00
CRDT- 2019/08/05 06:00
PHST- 2019/03/19 00:00 [received]
PHST- 2019/07/22 00:00 [revised]
PHST- 2019/07/25 00:00 [accepted]
PHST- 2019/08/05 06:00 [pubmed]
PHST- 2020/02/07 06:00 [medline]
PHST- 2019/08/05 06:00 [entrez]
AID - S0065-1281(19)30088-1 [pii]
AID - 10.1016/j.acthis.2019.07.008 [doi]
PST - ppublish
SO  - Acta Histochem. 2019 Oct;121(7):823-832. doi: 10.1016/j.acthis.2019.07.008. Epub 
      2019 Jul 31.