PMID- 31377732
OWN - NLM
STAT- MEDLINE
DCOM- 20200917
LR  - 20210213
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 57
IP  - 12
DP  - 2019 Nov 26
TI  - Measurement of alpha-dicarbonyl compounds in human saliva by pre-column 
      derivatization HPLC.
PG  - 1915-1922
LID - 10.1515/cclm-2019-0350 [doi]
AB  - Background alpha-Dicarbonyl compounds (alpha-DCs) have been detected in body fluids 
      including plasma and urine and elevation of this sort of compounds in vivo has 
      been associated with the development of many kinds of chronic diseases. However 
      whether alpha-DCs are present in human saliva, and if their presence/absence can be 
      related with various chronic diseases is yet to be determined. Methods In this 
      study, a pre-column derivatization HPLC-UV method was developed to measure 
      3-deoxyglucosone (3-DG), glyoxal (GO), methylglyoxal (MGO), diacetyl (DA), and 
      pentane-2,3-dione (PD) in human saliva employing 
      4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine (DQB) as a derivatizing 
      reagent. The derivatization of the alpha-DCs is fast and the conditions are facile. 
      The method was evaluated and the results show that it is suitable for the 
      quantification of alpha-DCs in human saliva. Results In the measurements of these 
      alpha-DCs in the saliva of 15 healthy subjects and 23 type 2 diabetes mellitus (T2DM) 
      patients, we found that the concentrations of GO and MGO in the saliva of the 
      diabetic patients were significantly higher than those in healthy subjects. As 
      far as we know, this is the first time that salivary alpha-DC concentrations have 
      been determined and associated with T2DM. Conclusions The developed method would 
      be useful for the measurement of the salivary alpha-DC levels and the data acquired 
      could be informative in the early screening for diabetes.
FAU - Wang, Xin-Jie
AU  - Wang XJ
AD  - National Engineering Research Center for Miniaturized Detection Systems and 
      College of Life Sciences, Northwest University, Xi'an, Shaanxi, P.R. China.
FAU - Zhang, Hong-Xia
AU  - Zhang HX
AD  - National Engineering Research Center for Miniaturized Detection Systems and 
      College of Life Sciences, Northwest University, Xi'an, Shaanxi, P.R. China.
FAU - Li, Heng
AU  - Li H
AD  - National Engineering Research Center for Miniaturized Detection Systems and 
      College of Life Sciences, Northwest University, Xi'an, Shaanxi, P.R. China.
FAU - Zhu, Ai-Hua
AU  - Zhu AH
AD  - The Shaanxi Key Laboratory of Chinese Medicine Research and Development, Xi'an, 
      P.R. China.
FAU - Gao, Wen-Yun
AU  - Gao WY
AD  - National Engineering Research Center for Miniaturized Detection Systems and 
      College of Life Sciences, Northwest University, 229 North Taibai Road, Xi'an, 
      Shaanxi 710069, P.R. China.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 50NP6JJ975 (Glyoxal)
RN  - 722KLD7415 (Pyruvaldehyde)
RN  - 9G2MP84A8W (Deoxyglucose)
RN  - EXV5374VEY (3-deoxyglucosone)
SB  - IM
MH  - Adult
MH  - Chromatography, High Pressure Liquid/methods
MH  - Deoxyglucose/*analogs & derivatives/analysis
MH  - Diabetes Mellitus, Type 2/blood
MH  - Female
MH  - Glyoxal/*analysis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pyruvaldehyde/*analysis
MH  - Saliva/chemistry
OTO - NOTNLM
OT  - HPLC determination
OT  - diabetes
OT  - saliva
OT  - alpha-dicarbonyl compounds
EDAT- 2019/08/05 06:00
MHDA- 2020/09/18 06:00
CRDT- 2019/08/05 06:00
PHST- 2019/01/02 00:00 [received]
PHST- 2019/06/17 00:00 [accepted]
PHST- 2019/08/05 06:00 [pubmed]
PHST- 2020/09/18 06:00 [medline]
PHST- 2019/08/05 06:00 [entrez]
AID - cclm-2019-0350 [pii]
AID - 10.1515/cclm-2019-0350 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2019 Nov 26;57(12):1915-1922. doi: 10.1515/cclm-2019-0350.