PMID- 31377977
OWN - NLM
STAT- MEDLINE
DCOM- 20200309
LR  - 20220125
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 5
DP  - 2019 Oct
TI  - Association of endothelial nitric oxide synthase (eNOS) and norepinephrine 
      transporter (NET) genes polymorphism with type 2 diabetes mellitus.
PG  - 5433-5441
LID - 10.1007/s11033-019-04998-y [doi]
AB  - Genetic factors in combination with environmental factors play a critical role in 
      the development type 2 diabetes mellitus (T2DM) which is growing as an epidemic 
      globally. In present study we aim to assess the association of eNOS (G894T, 
      rs1799983) and NET (G1287A, rs5569) genes polymorphism with T2DM. A case-control 
      study including a total of 400 North Indian subjects (200 T2DM cases and 200 
      controls) was performed using the polymerase chain reaction-restriction fragment 
      length polymorphism (PCR-RFLP) approach to analyze genetic polymorphism. 
      Alleles/genotype frequencies between cases and controls were compared using chi(2) 
      and Student's t-tests. Odds ratios and 95% confidence intervals were calculated 
      by logistic regression to assess the relative association between disease and 
      genotypes. In case of NET gene, GG (P = 0.002 in T2DM males, 0.053 in overall 
      T2DM cases) genotype and G allele (P = 0.003 in T2DM males, 0.027 in overall T2DM 
      cases) were found to be a positive risk factors and AG genotype (P = 0.012 in 
      T2DM males) and A allele (P = 0.003 in T2DM males, P = 0.027 in overall T2DM 
      cases) as negative risk factor for T2DM. No association of eNOS gene polymorphism 
      was found with T2DM (P values of all genotypes and alleles were greater than 
      0.05). NET gene polymorphism might be associated with the risk of T2DM whereas; 
      eNOS gene polymorphism do not confer any risk of T2DM in North Indian Ethnic 
      group. It is hoped that understanding genetic causes of T2DM will lead to earlier 
      diagnosis, preventive measures and more effective and specific treatment.
FAU - Rizvi, Saliha
AU  - Rizvi S
AD  - Molecular Biology Lab, Department of Biochemistry, Era's Lucknow Medical College 
      and Hospital, Lucknow, Uttar Pradesh, 226003, India.
FAU - Raza, Syed Tasleem
AU  - Raza ST
AD  - Molecular Biology Lab, Department of Biochemistry, Era's Lucknow Medical College 
      and Hospital, Lucknow, Uttar Pradesh, 226003, India. tasleem24@gmail.com.
FAU - Rahman, Qamar
AU  - Rahman Q
AD  - Amity University, Lucknow Campus, Lucknow, India.
FAU - Eba, Ale
AU  - Eba A
AD  - Molecular Biology Lab, Department of Biochemistry, Era's Lucknow Medical College 
      and Hospital, Lucknow, Uttar Pradesh, 226003, India.
FAU - Zaidi, Zeashan H
AU  - Zaidi ZH
AD  - Department of Statistics, Era's Lucknow Medical College and Hospital, Lucknow, 
      India.
FAU - Mahdi, Farzana
AU  - Mahdi F
AD  - Molecular Biology Lab, Department of Biochemistry, Era's Lucknow Medical College 
      and Hospital, Lucknow, Uttar Pradesh, 226003, India.
LA  - eng
PT  - Journal Article
DEP - 20190803
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Norepinephrine Plasma Membrane Transport Proteins)
RN  - 0 (SLC6A2 protein, human)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/enzymology/*genetics/metabolism
MH  - Female
MH  - Gene Frequency
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nitric Oxide Synthase Type III/*genetics/metabolism
MH  - Norepinephrine Plasma Membrane Transport Proteins/*genetics/metabolism
MH  - Polymorphism, Restriction Fragment Length
MH  - Polymorphism, Single Nucleotide
MH  - Risk Factors
OTO - NOTNLM
OT  - Case-control study
OT  - Gene polymorphism
OT  - NET
OT  - Type 2 diabetes mellitus
OT  - eNOS
EDAT- 2019/08/05 06:00
MHDA- 2020/03/10 06:00
CRDT- 2019/08/05 06:00
PHST- 2019/04/25 00:00 [received]
PHST- 2019/07/23 00:00 [accepted]
PHST- 2019/08/05 06:00 [pubmed]
PHST- 2020/03/10 06:00 [medline]
PHST- 2019/08/05 06:00 [entrez]
AID - 10.1007/s11033-019-04998-y [pii]
AID - 10.1007/s11033-019-04998-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Oct;46(5):5433-5441. doi: 10.1007/s11033-019-04998-y. Epub 
      2019 Aug 3.