PMID- 31378908
OWN - NLM
STAT- MEDLINE
DCOM- 20201015
LR  - 20201015
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 15
DP  - 2019 Aug
TI  - Effect of miR-124 on neuronal apoptosis in rats with cerebral infarction through 
      Wnt/beta-catenin signaling pathway.
PG  - 6657-6664
LID - 18556 [pii]
LID - 10.26355/eurrev_201908_18556 [doi]
AB  - OBJECTIVE: The aim of this study was to observe the influence of micro 
      ribonucleic acid (miR)-124 on neuronal apoptosis in rats with cerebral infarction 
      (CI), and to further investigate the underlying mechanism of miR-124 in CI 
      occurrence and development. MATERIALS AND METHODS: A total of 60 adult male 
      Wistar rats were randomly divided into the Sham group, the CI group and the CI + 
      miR-124 mimics group using a random number table. The focal CI model was 
      established using the suture-occluded method. After successful modeling, miR-124 
      mimics were stereotactically injected into the lateral ventricle of rats. 24 h 
      after operation, the neurological function of rats in each group was scored using 
      modified neurological severity score (mNSS). Meanwhile, the infarction area of 
      brain tissues was evaluated by the triphenyl tetrazolium chloride (TTC) method. 
      The protein expression levels of apoptosis-related genes, including B-cell 
      lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), C-Caspase and T-Caspase, 
      were detected via Western blotting. The expression and location of caspase-3 in 
      brain tissues were detected via immunofluorescence staining. Moreover, the level 
      of apoptosis in each group was detected via terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay. In addition, the 
      expression levels of the Wnt/beta-catenin signaling pathway-related proteins were 
      detected via Western blotting. RESULTS: Polymerase Chain Reaction (PCR) results 
      revealed that the expression level of miR-124 in the CI group was significantly 
      decreased when compared with that of the Sham group (p<0.05). The mNSS and TTC 
      staining results manifested that injection of miR-124 mimics could significantly 
      reduce the CI-induced neurological deficits and CI area (p<0.05). At the same 
      time, the levels of Bax and C-caspase/T-Caspase were significantly decreased, 
      whereas the expression of Bcl-2 was remarkably increased after the injection of 
      miR-124 mimics (p<0.05). Besides, the number of apoptotic cells in the CI + 
      miR-124 mimic group was remarkably decreased (p<0.05). In addition, miR-124 
      mimics significantly activated the expression levels of Wnt and beta-catenin 
      (p<0.05). CONCLUSIONS: The inhibitory effect of miR-124 on neuronal apoptosis in 
      CI rats is probably related to the activation of the Wnt/beta-catenin signaling 
      pathway. Furthermore, miR-124 is expected to be a target drug for the clinical 
      treatment of CI.
FAU - Che, Q-Q
AU  - Che QQ
AD  - Department of Neurology, Cixi People's Hospital, Ningbo, China. 
      1091374055@qq.com.
FAU - Huang, T
AU  - Huang T
FAU - Zhang, Y-D
AU  - Zhang YD
FAU - Qian, X-J
AU  - Qian XJ
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN124 microRNA, rat)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Brain/*pathology
MH  - Cerebral Infarction/diagnosis/genetics/*pathology/therapy
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Humans
MH  - Injections, Intraventricular
MH  - Male
MH  - MicroRNAs/administration & dosage/analysis/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Severity of Illness Index
MH  - Stereotaxic Techniques
MH  - Wnt Signaling Pathway/*genetics
EDAT- 2019/08/06 06:00
MHDA- 2020/10/21 06:00
CRDT- 2019/08/06 06:00
PHST- 2019/08/06 06:00 [entrez]
PHST- 2019/08/06 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 18556 [pii]
AID - 10.26355/eurrev_201908_18556 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Aug;23(15):6657-6664. doi: 
      10.26355/eurrev_201908_18556.