PMID- 31382405
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 11
IP  - 8
DP  - 2019 Aug 2
TI  - Sustained Release of Minor-Groove-Binding Antibiotic Netropsin from 
      Calcium-Coated Groove-Rich DNA Particles.
LID - 10.3390/pharmaceutics11080387 [doi]
LID - 387
AB  - Control of the release properties of drugs has been considered a key factor in 
      the development of drug delivery systems (DDSs). However, drug delivery has 
      limitations including cytotoxicity, low loading efficiency, and burst release. To 
      overcome these challenges, nano or micro-particles have been suggested as carrier 
      systems to deliver chemical drugs. Herein, nano-sized DNA particles (DNAp) were 
      manufactured to deliver netropsin, which is known to bind to DNA minor grooves. 
      The rationally designed particles with exposed rich minor grooves were prepared 
      by DNAp synthesis via rolling circle amplification (RCA). DNAp could load large 
      quantities of netropsin in its minor grooves. An analytical method was also 
      developed for the quantification of netropsin binding to DNAp by UV-visible 
      spectrometry. Moreover, controlled release of netropsin was achieved by forming a 
      layer of Ca(2+) on the DNAp (CaDNAp). As a proof of concept, the sustained 
      release of netropsin by CaDNAp highlights the potential of the DNAp-based 
      delivery approach.
FAU - Jeon, Hyunsu
AU  - Jeon H
AD  - Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, 
      Dongdaemungu, Seoul 02504, Korea.
FAU - Nam, Hyangsu
AU  - Nam H
AD  - Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, 
      Dongdaemungu, Seoul 02504, Korea.
FAU - Lee, Jong Bum
AU  - Lee JB
AD  - Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, 
      Dongdaemungu, Seoul 02504, Korea. jblee@uos.ac.kr.
LA  - eng
GR  - 2017M3D1A1039423/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20190802
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC6724015
OTO - NOTNLM
OT  - DNA particle (DNAp)
OT  - calcium
OT  - minor groove binder
OT  - netropsin
OT  - rolling circle amplification (RCA)
COIS- The authors declare no conflict of interest.
EDAT- 2019/08/07 06:00
MHDA- 2019/08/07 06:01
PMCR- 2019/08/01
CRDT- 2019/08/07 06:00
PHST- 2019/05/20 00:00 [received]
PHST- 2019/07/14 00:00 [revised]
PHST- 2019/07/22 00:00 [accepted]
PHST- 2019/08/07 06:00 [entrez]
PHST- 2019/08/07 06:00 [pubmed]
PHST- 2019/08/07 06:01 [medline]
PHST- 2019/08/01 00:00 [pmc-release]
AID - pharmaceutics11080387 [pii]
AID - pharmaceutics-11-00387 [pii]
AID - 10.3390/pharmaceutics11080387 [doi]
PST - epublish
SO  - Pharmaceutics. 2019 Aug 2;11(8):387. doi: 10.3390/pharmaceutics11080387.