PMID- 31382472
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 9
IP  - 8
DP  - 2019 Aug 2
TI  - Global Mapping of H3K4 Trimethylation (H3K4me3) and Transcriptome Analysis Reveal 
      Genes Involved in the Response to Epidemic Diarrhea Virus Infections in Pigs.
LID - 10.3390/ani9080523 [doi]
LID - 523
AB  - Porcine epidemic diarrhea virus (PEDV) is currently detected as the main pathogen 
      causing severe diarrhea in pig farms. The phenotypic alterations induced by 
      pathogenic infections are usually tightly linked with marked changes in 
      epigenetic modification and gene expression. We performed global mapping of H3K4 
      trimethylation (H3K4me3) and transcriptomic analyses in the jejunum of 
      PEDV-infected and healthy piglets using chromatin immunoprecipitation sequencing 
      and RNA-seq techniques. A total of 1885 H3K4me3 peaks that are associated with 
      1723 genes were characterized. Moreover, 290 differentially expressed genes were 
      identified, including 104 up-regulated and 186 down-regulated genes. Several 
      antiviral genes including 2'-5'-oligoadenylate synthetase 1 (OAS1), 
      2'-5'-oligoadenylate synthetase 2 (OAS2), ephrin B2 (EFNB2), and CDC28 protein 
      kinase regulatory subunit 1B (CKS1B) with higher H3K4me3 enrichment and 
      expression levels in PEDV-infected samples suggested the potential roles of 
      H3K4me3 deposition in promoting their expressions. Transcription factor 
      annotation analysis highlighted the potential roles of two transcription factors 
      interferon regulatory factor 8 (IRF8) and Kruppel like factor 4 (KLF4) in 
      modulating the differential expression of genes involved in PEDV infection. The 
      results provided novel insights into PEDV infection from the transcriptomic and 
      epigenetic layers and revealed previously unknown and intriguing elements 
      potentially involved in the host responses.
FAU - Wang, Haifei
AU  - Wang H
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, 
      College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
FAU - Yang, Li
AU  - Yang L
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, 
      College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
FAU - Qu, Huan
AU  - Qu H
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, 
      College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
FAU - Feng, Haiyue
AU  - Feng H
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, 
      College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
FAU - Wu, Shenglong
AU  - Wu S
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, 
      College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China.
FAU - Bao, Wenbin
AU  - Bao W
AD  - Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, 
      College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China. wbbao@yzu.edu.cn.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. wbbao@yzu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190802
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC6719071
OTO - NOTNLM
OT  - H3K4me3
OT  - PEDV
OT  - gene expression
OT  - pig
OT  - transcriptional regulation
COIS- The authors declare that there is no conflict of interest.
EDAT- 2019/08/07 06:00
MHDA- 2019/08/07 06:01
PMCR- 2019/08/02
CRDT- 2019/08/07 06:00
PHST- 2019/06/12 00:00 [received]
PHST- 2019/07/29 00:00 [revised]
PHST- 2019/07/30 00:00 [accepted]
PHST- 2019/08/07 06:00 [entrez]
PHST- 2019/08/07 06:00 [pubmed]
PHST- 2019/08/07 06:01 [medline]
PHST- 2019/08/02 00:00 [pmc-release]
AID - ani9080523 [pii]
AID - animals-09-00523 [pii]
AID - 10.3390/ani9080523 [doi]
PST - epublish
SO  - Animals (Basel). 2019 Aug 2;9(8):523. doi: 10.3390/ani9080523.