PMID- 31382976
OWN - NLM
STAT- MEDLINE
DCOM- 20200709
LR  - 20211204
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 17
IP  - 1
DP  - 2019 Aug 5
TI  - Novel method for simultaneously detecting HPA and HLA antibodies using Luminex 
      microbeads.
PG  - 249
LID - 10.1186/s12967-019-2002-4 [doi]
LID - 249
AB  - BACKGROUND: Alloantibodies against human platelet antigens (HPAs) and human 
      leukocyte antigen (HLA) are implicated in several immune-mediated platelet 
      disorders. Detection of these antibodies is crucial in the diagnosis and 
      management of these disorders. The aim of this study was to establish a novel 
      method to simultaneously detect HPA-1, HPA-2, HPA-3, HPA-5 and HLA antibodies 
      with Luminex microbeads technology. METHODS: Monoclonal antibodies specific for 
      platelet glycoproteins and HLA class I molecules were separately coupled to the 
      Luminex microbeads. We validated specificity of the Luminex platform using the 
      following antibodies: anti-HPA-1a, anti-HPA-2b, anti-HPA-3a, anti-HPA-5a, and 
      anti-HLA positive samples. Sensitivity was evaluated by a serial dilution (from 
      neat to 1/1024) using the following antibodies: anti-HPA-1a, anti-HPA-3a standard 
      sera, and anti-HPA-5a positive serum. Serum samples were collected from 36 
      neonatal alloimmune thrombocytopenia (NAIT) patients suspected of having HPA or 
      HLA antibodies and 8 samples from ISBT platelet workshop were tested using the 
      Luminex assay. RESULTS: The Luminex assay detected all antibodies tested from the 
      known samples. The sensitivities of the Luminex assay detecting anti-HPA-1a, 
      anti-HPA-3a, and anti-HPA-5a were 1:512, 1:64, and 1:128, respectively. The 
      sensitivity of Luminex assay was higher than monoclonal antibody immobilization 
      of platelet antigen method (MAIPA). No cross-reactivity was observed in the 
      samples containing multi-platelet antibodies or mixture antibodies against HPA 
      and HLA. The results of 44 samples with platelet disorders were consistent with 
      those of the same samples processed with the MAIPA assay. CONCLUSION: Luminex 
      microbeads coupled with monoclonal antibodies could be successfully used to 
      detect HPA and HLA antibodies simultaneously, especially with high sensitivity in 
      detecting HPA antibodies.
FAU - Tao, Sudan
AU  - Tao S
AD  - Blood Center of Zhejiang Province, Jianye Road 789, Hangzhou, 310052, Zhejiang, 
      China.
AD  - Key Laboratory of Blood Safety Research, Zhejiang Province, Jianye Road 789, 
      Hangzhou, 310052, Zhejiang, China.
FAU - Chen, Shu
AU  - Chen S
AD  - Blood Center of Zhejiang Province, Jianye Road 789, Hangzhou, 310052, Zhejiang, 
      China.
AD  - Key Laboratory of Blood Safety Research, Zhejiang Province, Jianye Road 789, 
      Hangzhou, 310052, Zhejiang, China.
FAU - Hong, Xiaozhen
AU  - Hong X
AD  - Blood Center of Zhejiang Province, Jianye Road 789, Hangzhou, 310052, Zhejiang, 
      China.
AD  - Key Laboratory of Blood Safety Research, Zhejiang Province, Jianye Road 789, 
      Hangzhou, 310052, Zhejiang, China.
FAU - He, Ji
AU  - He J
AD  - Blood Center of Zhejiang Province, Jianye Road 789, Hangzhou, 310052, Zhejiang, 
      China.
AD  - Key Laboratory of Blood Safety Research, Zhejiang Province, Jianye Road 789, 
      Hangzhou, 310052, Zhejiang, China.
FAU - Zhu, Faming
AU  - Zhu F
AUID- ORCID: 0000-0002-1963-9176
AD  - Blood Center of Zhejiang Province, Jianye Road 789, Hangzhou, 310052, Zhejiang, 
      China. zfm00@hotmail.com.
AD  - Key Laboratory of Blood Safety Research, Zhejiang Province, Jianye Road 789, 
      Hangzhou, 310052, Zhejiang, China. zfm00@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190805
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (3a alloantigen, human)
RN  - 0 (5a alloantigen, human)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (HLA Antigens)
RN  - 0 (ITGB3 protein, human)
RN  - 0 (Integrin beta3)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, Human Platelet/*analysis
MH  - Blood Platelet Disorders/*immunology
MH  - Blood Platelets/cytology/*immunology
MH  - Genotype
MH  - HLA Antigens/*analysis
MH  - Humans
MH  - Immune System
MH  - Immunoassay/instrumentation/*methods
MH  - Integrin beta3
MH  - Isoantibodies
MH  - *Microspheres
MH  - Purpura, Thrombocytopenic, Idiopathic/blood
PMC - PMC6683390
OTO - NOTNLM
OT  - HLA antibodies
OT  - HPA antibodies
OT  - Luminex assay
OT  - MAIPA
COIS- The authors declare that they have no competing interests.
EDAT- 2019/08/07 06:00
MHDA- 2020/07/10 06:00
PMCR- 2019/08/05
CRDT- 2019/08/07 06:00
PHST- 2019/03/26 00:00 [received]
PHST- 2019/07/27 00:00 [accepted]
PHST- 2019/08/07 06:00 [entrez]
PHST- 2019/08/07 06:00 [pubmed]
PHST- 2020/07/10 06:00 [medline]
PHST- 2019/08/05 00:00 [pmc-release]
AID - 10.1186/s12967-019-2002-4 [pii]
AID - 2002 [pii]
AID - 10.1186/s12967-019-2002-4 [doi]
PST - epublish
SO  - J Transl Med. 2019 Aug 5;17(1):249. doi: 10.1186/s12967-019-2002-4.