PMID- 3138311
OWN - NLM
STAT- MEDLINE
DCOM- 19881025
LR  - 20151119
IS  - 0737-1454 (Print)
IS  - 0737-1454 (Linking)
VI  - 6
IP  - 4
DP  - 1988 Jul
TI  - The suppressive effects of recombinant human tumor necrosis factor-alpha on 
      normal and malignant myelopoiesis: synergism with interferon-gamma.
PG  - 241-61
AB  - The modulation of growth of normal and leukemic myeloid progenitor cells in soft 
      agar cultures by recombinant human tumor necrosis factor-alpha (TNF alpha) and 
      recombinant human interferon-gamma (IFN gamma) was investigated. TNF alpha 
      inhibited colony formation of all colony types representing different 
      maturational stages of normal progenitor cells committed to the myeloid lineage 
      with different orders of sensitivity. Blast-type colonies derived from patients 
      with acute myelogenous leukemia were more sensitive to TNF alpha inhibition than 
      progenitor cells purified from normal bone marrow or bone marrow from patients 
      with stable-phase chronic myelogenous leukemia. The response of most colony types 
      to IFN gamma was poor. However, when IFN gamma was administered together with TNF 
      alpha, synergistically enhanced antiproliferative effects were detected in all 
      colony types tested. The antiproliferative action of IFN gamma on myelopoiesis 
      was enhanced in culture by the presence of autologous monocytes, presumedly by 
      inducing endogenous production of TNF alpha. However, TNF alpha seemed to act 
      directly on the progenitor cells themselves to suppress their clonal growth, 
      rather than involving accessory marrow elements such as monocytes and/or T 
      lymphocytes.
FAU - Herrmann, F
AU  - Herrmann F
AD  - Department of Hematology, Johannes Gutenberg-University, Mainz, FRG.
FAU - Bambach, T
AU  - Bambach T
FAU - Bonifer, R
AU  - Bonifer R
FAU - Lindemann, A
AU  - Lindemann A
FAU - Riedel, D
AU  - Riedel D
FAU - Oster, W
AU  - Oster W
FAU - Mertelsmann, R
AU  - Mertelsmann R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cell Cloning
JT  - International journal of cell cloning
JID - 8308172
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Bone Marrow/*drug effects
MH  - Bone Marrow Cells
MH  - Drug Synergism
MH  - Hematopoietic Stem Cells/drug effects
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Leukemia, Myeloid/*pathology
MH  - Leukemia, Myeloid, Acute/*pathology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1988/07/01 00:00
MHDA- 1988/07/01 00:01
CRDT- 1988/07/01 00:00
PHST- 1988/07/01 00:00 [pubmed]
PHST- 1988/07/01 00:01 [medline]
PHST- 1988/07/01 00:00 [entrez]
AID - 10.1002/stem.5530060403 [doi]
PST - ppublish
SO  - Int J Cell Cloning. 1988 Jul;6(4):241-61. doi: 10.1002/stem.5530060403.