PMID- 31383894 OWN - NLM STAT- MEDLINE DCOM- 20201023 LR - 20210110 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 9 IP - 1 DP - 2019 Aug 5 TI - Characterization of subcutaneous and omental adipose tissue in patients with obesity and with different degrees of glucose impairment. PG - 11333 LID - 10.1038/s41598-019-47719-y [doi] LID - 11333 AB - Although obesity represents a risk factor for the development of type 2 diabetes mellitus (T2DM), the link between these pathological conditions is not so clear. The manner in which the different elements of adipose tissue (AT) interplay in order to grow has been suggested to have a role in the genesis of metabolic complications, but this has not yet been fully addressed in humans. Through IHC, transmission electron microscopy, cytometry, and in vitro cultures, we described the morphological and functional changes of subcutaneous and visceral AT (SAT and VAT) in normoglycemic, prediabetic and T2DM patients with obesity compared to lean subjects. In both SAT and VAT we measured a hypertrophic and hyperplastic expansion, causing similar vascular rarefaction in obese patients with different degrees of metabolic complications. Capillaries display dysfunctional basement membrane thickening only in T2DM patients evidencing VAT as a new target of T2DM microangiopathy. The largest increase in adipocyte size and decrease in adipose stem cell number and adipogenic potential occur both in T2DM and in prediabetes. We showed that SAT and VAT remodeling with stemness deficit is associated with early glucose metabolism impairment suggesting the benefit of an AT-target therapy controlling hypertrophy and hyperplasia already in prediabetic obese patients. FAU - Belligoli, Anna AU - Belligoli A AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Compagnin, Chiara AU - Compagnin C AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Sanna, Marta AU - Sanna M AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Favaretto, Francesca AU - Favaretto F AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Fabris, Roberto AU - Fabris R AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Busetto, Luca AU - Busetto L AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Foletto, Mirto AU - Foletto M AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Dal Pra, Chiara AU - Dal Pra C AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Serra, Roberto AU - Serra R AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Prevedello, Luca AU - Prevedello L AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Da Re, Chiara AU - Da Re C AD - Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Division of General Surgery, 35128, Padua, Italy. FAU - Bardini, Romeo AU - Bardini R AD - Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Division of General Surgery, 35128, Padua, Italy. FAU - Mescoli, Claudia AU - Mescoli C AD - Department of Medicine, University of Padua, Surgical Pathology and Cytopathology Unit, 35121, Padua, Italy. FAU - Rugge, Massimo AU - Rugge M AD - Department of Medicine, University of Padua, Surgical Pathology and Cytopathology Unit, 35121, Padua, Italy. FAU - Fioretto, Paola AU - Fioretto P AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. FAU - Conci, Scilla AU - Conci S AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Bettini, Silvia AU - Bettini S AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. FAU - Milan, Gabriella AU - Milan G AUID- ORCID: 0000-0001-8987-5310 AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. gabriella.milan@unipd.it. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. gabriella.milan@unipd.it. FAU - Vettor, Roberto AU - Vettor R AD - Department of Medicine, University of Padua, Internal Medicine 3, 35128, Padua, Italy. AD - Center for the Study and the Integrated Treatment of Obesity, Padua Hospital, 35128, Padua, Italy. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190805 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - IY9XDZ35W2 (Glucose) SB - IM MH - Abdominal Fat/metabolism/*pathology MH - Adult MH - Diabetes Mellitus, Type 2/complications/metabolism/*pathology MH - Female MH - Glucose/*metabolism MH - Humans MH - Male MH - Middle Aged MH - Obesity/complications/metabolism/*pathology MH - Subcutaneous Fat/metabolism/*pathology PMC - PMC6683173 COIS- The authors declare no competing interests. EDAT- 2019/08/07 06:00 MHDA- 2020/10/24 06:00 PMCR- 2019/08/05 CRDT- 2019/08/07 06:00 PHST- 2019/04/01 00:00 [received] PHST- 2019/07/12 00:00 [accepted] PHST- 2019/08/07 06:00 [entrez] PHST- 2019/08/07 06:00 [pubmed] PHST- 2020/10/24 06:00 [medline] PHST- 2019/08/05 00:00 [pmc-release] AID - 10.1038/s41598-019-47719-y [pii] AID - 47719 [pii] AID - 10.1038/s41598-019-47719-y [doi] PST - epublish SO - Sci Rep. 2019 Aug 5;9(1):11333. doi: 10.1038/s41598-019-47719-y.