PMID- 31384171
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220409
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 19
DP  - 2019
TI  - Upregulation of circFLNA contributes to laryngeal squamous cell carcinoma 
      migration by circFLNA-miR-486-3p-FLNA axis.
PG  - 196
LID - 10.1186/s12935-019-0924-9 [doi]
LID - 196
AB  - BACKGROUND: Accumulating evidence shows that circular RNAs (circRNAs) plays vital 
      roles in tumor progression. However, the biological functions of circRNAs in 
      laryngeal squamous cell carcinoma (LSCC) metastasis is still unclear. METHODS: 
      qRT-PCR was used to detect circFLNA, miRNAs and FLNA mRNA expression. Transwell 
      assay and western blot were performed to evaluate cell migration ability and to 
      detect FLNA, MMP2 and MLK1 protein expression, respectively. RNA pull-down 
      analysis was used to find the binding-miRNAs of circFLNA. Luciferase reporter 
      assay was used to examine the effect of circFLNA on miRNAs and miR-486-3p on FLNA 
      expression. RESULTS: In this study, we confirmed that a Filamin A (FLNA)-derived 
      hsa_circ_0092012 known as circFLNA, was upregulated in LSCC, and the higher 
      expression of circFLNA was correlated with LSCC lymph node metastasis. Increased 
      circFLNA facilitates LSCC cell migration ability through upregulating FLNA and 
      MMP2 protein expression. Mechanistically, we find that circFLNA sponges 
      miR-486-3p in LSCC cells, relieving miR-486-3p-induced repression of FLNA which 
      promotes LSCC cell migration. Accordingly, FLNA mRNA is overexpressed in LSCC 
      tissues and a higher FLNA level is correlated with poor survival. Dysregulation 
      of the circFLNA/miR-486-3p/FLNA regulatory pathway contributes to LSCC migration. 
      CONCLUSIONS: In summary, our study sheds light on the regulatory mechanism of 
      circFLNA in LSCC migration via sponging miR-486-3p, which downregulates the FLNA 
      protein expression. Targeting circFLNA/miR-486-3p/FLAN axis provides a potential 
      therapeutic target for aggressive LSCC.
FAU - Wang, Jian-Xing
AU  - Wang JX
AD  - 1Department of Pathology, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017 People's Republic of China. ISNI: 0000 0004 1760 8442. GRID: 
      grid.256883.2
AD  - 2Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Liu, Yan
AU  - Liu Y
AD  - 1Department of Pathology, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017 People's Republic of China. ISNI: 0000 0004 1760 8442. GRID: 
      grid.256883.2
AD  - 3Department of Anesthesiology, The 4th Hospital of Hebei Medical University, 169 
      Tianshan Street, 050000 Shijiazhuang, People's Republic of China. ISNI: 0000 0004 
      1760 8442. GRID: grid.256883.2
FAU - Jia, Xin-Ju
AU  - Jia XJ
AD  - 4Departmen of Endocrinology, The First Hospital of Hebei Medical University, 89 
      Donggang Road, Shijiazhuang, 050000 People's Republic of China. GRID: 
      grid.452458.a
FAU - Liu, Shu-Xia
AU  - Liu SX
AD  - 2Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Dong, Jin-Hui
AU  - Dong JH
AD  - 2Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Ren, Xiu-Min
AU  - Ren XM
AD  - 2Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Xu, Ou
AU  - Xu O
AD  - 2Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Zhang, Hai-Zhong
AU  - Zhang HZ
AD  - 2Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
FAU - Duan, Hui-Jun
AU  - Duan HJ
AD  - 1Department of Pathology, Hebei Medical University, 361 Zhongshan East Road, 
      Shijiazhuang, 050017 People's Republic of China. ISNI: 0000 0004 1760 8442. GRID: 
      grid.256883.2
FAU - Shan, Chun-Guang
AU  - Shan CG
AD  - 2Department of Otolaryngology, The Second Hospital of Hebei Medical University, 
      215 Heping West Road, Shijiazhuang, 050000 People's Republic of China. ISNI: 0000 
      0004 1804 3009. GRID: grid.452702.6
LA  - eng
PT  - Journal Article
DEP - 20190729
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC6664525
OTO - NOTNLM
OT  - FLNA
OT  - Laryngeal squamous cell carcinoma
OT  - Migration
OT  - circRNAs
OT  - miRNA
COIS- Competing interestsThe authors declare that they have no competing interests.
EDAT- 2019/08/07 06:00
MHDA- 2019/08/07 06:01
PMCR- 2019/07/29
CRDT- 2019/08/07 06:00
PHST- 2019/04/23 00:00 [received]
PHST- 2019/07/22 00:00 [accepted]
PHST- 2019/08/07 06:00 [entrez]
PHST- 2019/08/07 06:00 [pubmed]
PHST- 2019/08/07 06:01 [medline]
PHST- 2019/07/29 00:00 [pmc-release]
AID - 924 [pii]
AID - 10.1186/s12935-019-0924-9 [doi]
PST - epublish
SO  - Cancer Cell Int. 2019 Jul 29;19:196. doi: 10.1186/s12935-019-0924-9. eCollection 
      2019.