PMID- 31384983
OWN - NLM
STAT- MEDLINE
DCOM- 20191119
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 411
IP  - 25
DP  - 2019 Oct
TI  - Electrochemical aptasensor for the detection of HER2 in human serum to assist in 
      the diagnosis of early stage breast cancer.
PG  - 6667-6676
LID - 10.1007/s00216-019-02040-5 [doi]
AB  - Human epidermal growth factor receptor-2 (HER2) is an important biomarker in the 
      diagnosis and prognosis of breast cancer. This work aimed to develop an 
      aptasensor to detect HER2 in human serum. HER2 aptamer was immobilized by 
      electrostatic adsorption on the surface of a homemade screen-printed electrode 
      modified with poly-L-lysine. Measurements were made by differential pulse 
      voltammetry using methylene blue as a redox indicator. A calibration curve was 
      constructed (R(2) = 0.997) using different concentrations of HER2 protein (10-60 
      ng/mL) in PBS buffer (pH 7.4), with a detection limit of 3.0 ng/mL. The 
      aptasensor showed good reproducibility with relative standard deviations (RSDs) 
      of 3% and remained stable for 3 days with an RSD around 2%. When the tests were 
      performed with serum from a healthy woman, a peak of 6.72 muA was found without 
      the addition of the protein. When we tested the serum of a woman with HER2+ 
      breast cancer, we obtained a signal of 2.65 muA; the same pattern was found when 
      adding to protein in serum control, i.e., the higher the concentration of 
      protein, the lower the signal. The aptasensor was characterized by scanning 
      electron microscopy and isothermal titration calorimetry (ITC), showing excellent 
      interaction between aptamer and target protein. The results revealed a promising 
      and sensitive tool capable of detecting HER2 protein in human serum with albumin 
      depletion, aiding in the molecular diagnosis of breast cancer. Graphical 
      abstract.
FAU - Bezerra, Giselda
AU  - Bezerra G
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil. 
      giseldamilamari@hotmail.com.
FAU - Cordula, Carolina
AU  - Cordula C
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Campos, Danielly
AU  - Campos D
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Nascimento, Gustavo
AU  - Nascimento G
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Oliveira, Natalia
AU  - Oliveira N
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Seabra, Maria Aparecida
AU  - Seabra MA
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Visani, Valeria
AU  - Visani V
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Lucas, Sampaio
AU  - Lucas S
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Lopes, Iasmim
AU  - Lopes I
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Santos, Joana
AU  - Santos J
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Xavier, Francisco Jr
AU  - Xavier F Jr
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Borba, Maria Amelia
AU  - Borba MA
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Martins, Danyelly
AU  - Martins D
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
FAU - Lima-Filho, Jose
AU  - Lima-Filho J
AD  - Laboratorio de Imunopatologia Keizo Asami - LIKA, Universidade Federal de 
      Pernambuco - UFPE, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20190805
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Aptamers, Nucleotide)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/instrumentation/methods
MH  - Breast Neoplasms/*blood/diagnosis
MH  - Early Detection of Cancer
MH  - Electrochemical Techniques/instrumentation/methods
MH  - Electrodes
MH  - Equipment Design
MH  - Female
MH  - Humans
MH  - Limit of Detection
MH  - Receptor, ErbB-2/analysis/*blood
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Aptamer
OT  - Aptasensor
OT  - Breast cancer
OT  - Diagnosis
OT  - HER2
OT  - Screen-printed electrode
EDAT- 2019/08/07 06:00
MHDA- 2019/11/20 06:00
CRDT- 2019/08/07 06:00
PHST- 2019/05/04 00:00 [received]
PHST- 2019/07/17 00:00 [accepted]
PHST- 2019/07/07 00:00 [revised]
PHST- 2019/08/07 06:00 [pubmed]
PHST- 2019/11/20 06:00 [medline]
PHST- 2019/08/07 06:00 [entrez]
AID - 10.1007/s00216-019-02040-5 [pii]
AID - 10.1007/s00216-019-02040-5 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2019 Oct;411(25):6667-6676. doi: 10.1007/s00216-019-02040-5. 
      Epub 2019 Aug 5.