PMID- 31387006
OWN - NLM
STAT- MEDLINE
DCOM- 20200204
LR  - 20200204
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 118
DP  - 2019 Oct
TI  - The protective effect of icariin and phosphorylated icariin against LPS-induced 
      intestinal epithelial cells injury.
PG  - 109246
LID - S0753-3322(19)32344-3 [pii]
LID - 10.1016/j.biopha.2019.109246 [doi]
AB  - Icariin (ICA) and phosphorylated icariin (pICA) have excellent antiviral and 
      antioxidant effects. However, whether ICA and pICA cause anti-LPS-induced 
      intestinal damage remains unclear. In this study, we used Caco-2 cells as a model 
      to investigate the protective effects of ICA and pICA on human colonic epithelial 
      cells and explore their potential mechanisms. Our results indicated that ICA and 
      pICA increased cell viability and decreased lactate dehydrogenase activity in 
      Caco-2 cells. ICA and pICA also attenuated LPS-induced changes in intestinal 
      epithelial cell permeability and reduced the levels of oxidative stress 
      indicators, such as reactive oxygen species, malondialdehyde, and hydrogen 
      peroxide, in Caco-2 cells. Antioxidant indicators, such as superoxide dismutase, 
      glutathione peroxidase, catalase and total antioxidant capacity, were increased, 
      while the levels of IL-1beta, IL-6, IL-8 and TNF-alpha were reduced in the ICA and pICA 
      groups. Furthermore, ICA and pICA decreased the gene abundance and enzyme 
      activities of caspase-3, -8, -9 and -10 in Caco-2 cells. Our data suggest that 
      ICA and pICA effectively attenuated LPS-induced changes in the oxidative stress, 
      inflammation, apoptosis and intestinal permeability of intestinal epithelial 
      cells. These findings provide new insight for treating LPS-induced intestinal 
      injury.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Masson SAS.. All rights 
      reserved.
FAU - Xiong, Wen
AU  - Xiong W
AD  - College of Animal Science and Technology, Southwest University, Chongqing, China. 
      Electronic address: pansquito@hotmail.com.
FAU - Ma, Haoyue
AU  - Ma H
AD  - College of Pharmaceutical Sciences, Southwest University, Chongqing, China. 
      Electronic address: mhy950708@vip.qq.com.
FAU - Zhang, Zhu
AU  - Zhang Z
AD  - College of Animal Science and Technology, Southwest University, Chongqing, China. 
      Electronic address: zhuzi625@sina.com.
FAU - Jin, Meilan
AU  - Jin M
AD  - College of Animal Science and Technology, Southwest University, Chongqing, China. 
      Electronic address: meilan0622@swu.edu.cn.
FAU - Wang, Jian
AU  - Wang J
AD  - College of Animal Science and Technology, Southwest University, Chongqing, China. 
      Electronic address: wj0931@swu.edu.cn.
FAU - Xu, Yuwei
AU  - Xu Y
AD  - College of Animal Science and Technology, Southwest University, Chongqing, China. 
      Electronic address: xuyuwei@swu.edu.cn.
FAU - Wang, Zili
AU  - Wang Z
AD  - College of Animal Science and Technology, Southwest University, Chongqing, China. 
      Electronic address: wzl9698@126.com.
LA  - eng
PT  - Journal Article
DEP - 20190803
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Flavonoids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protective Agents)
RN  - 0 (Transcription Factor RelA)
RN  - VNM47R2QSQ (icariin)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Caco-2 Cells
MH  - Cell Survival/drug effects
MH  - Epithelial Cells/drug effects/*pathology
MH  - Flavonoids/*pharmacology
MH  - Humans
MH  - Inflammation/pathology
MH  - Intestines/*pathology
MH  - Lipopolysaccharides
MH  - Oxidation-Reduction
MH  - Phosphorylation/drug effects
MH  - Protective Agents/*pharmacology
MH  - Transcription Factor RelA/metabolism
OTO - NOTNLM
OT  - Caco-2 cells
OT  - Icariin
OT  - Intestinal injury
OT  - LPS
OT  - Phosphorylated icariin
EDAT- 2019/08/07 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/08/07 06:00
PHST- 2019/05/20 00:00 [received]
PHST- 2019/07/17 00:00 [revised]
PHST- 2019/07/18 00:00 [accepted]
PHST- 2019/08/07 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/08/07 06:00 [entrez]
AID - S0753-3322(19)32344-3 [pii]
AID - 10.1016/j.biopha.2019.109246 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2019 Oct;118:109246. doi: 10.1016/j.biopha.2019.109246. Epub 
      2019 Aug 3.