PMID- 31387799 OWN - NLM STAT- MEDLINE DCOM- 20200311 LR - 20201209 IS - 2468-0869 (Electronic) IS - 2468-0451 (Linking) VI - 24 IP - 4 DP - 2019 Nov TI - Microbiological evaluation of the ability of the DEKO-190 Washer/Disinfector to remove Clostridium difficile spores from bedpan surfaces. PG - 208-211 LID - S2468-0451(19)30037-9 [pii] LID - 10.1016/j.idh.2019.07.001 [doi] AB - BACKGROUND: Clostridium difficile is a major nosocomial pathogen causing mild diarrhoea to life-threatening pseudomembranous colitis, and its spores frequently contaminate hospital environments and equipment. Washer/Disinfectors (WDs) are commonly used to clean and decontaminate soiled equipment in health care facilities. This study aimed to evaluate the effectiveness of the DEKO-190 WD in removing C. difficile spores from bedpans. METHODS: Plastic carriers were inoculated with suspensions of C. difficile spores in autoclaved (sterile) human faeces. The carriers were then taped to a sterile plastic bedpan which was subjected to short, long or intensive wash cycles in the WD using one of two test detergents: Formula A (generic) and Formula B (highly alkaline). Mean log(10) reductions in spores were calculated for each wash cycle. RESULTS: Mean log(10) reductions were 3.21(SEM +/- 0.20) and 2.82 (+/-0.13) for Formula A and B, respectively, for the short cycle. The mean log(10) reductions using the long wash cycle were 3.65 (+/-0.44) using Formula A and 5.30 (+/-0.43) using Formula B, while log(10) reductions were 3.37 (+/-0.58) (Formula A) and 4.64 (+/-0.47) (Formula B) for the intensive cycle. Washing with the DEKO-190 significantly reduced spore concentrations on carrier surfaces on a bedpan. Spore counts were most effectively reduced when carriers were washed on a long or intensive wash cycle using an alkaline detergent. CI - Copyright (c) 2019 Australasian College for Infection Prevention and Control. Published by Elsevier B.V. All rights reserved. FAU - Collins, Deirdre A AU - Collins DA AD - School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Australia; School of Medical & Health Sciences, Edith Cowan University, Joondalup, Australia. Electronic address: deirdre.collins@ecu.edu.au. FAU - Carson, Kerry C AU - Carson KC AD - Department of Microbiology, PathWest Laboratory Medicine, Nedlands, Australia. FAU - Riley, Thomas V AU - Riley TV AD - School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Australia; School of Medical & Health Sciences, Edith Cowan University, Joondalup, Australia; Department of Microbiology, PathWest Laboratory Medicine, Nedlands, Australia; School of Veterinary & Life Sciences, Murdoch University, Murdoch, Australia. LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190803 PL - Netherlands TA - Infect Dis Health JT - Infection, disease & health JID - 101689703 RN - 0 (Disinfectants) SB - IM MH - Clostridioides difficile/drug effects/*growth & development MH - Clothing MH - Colony Count, Microbial MH - Cross Infection/microbiology/prevention & control MH - Disinfectants/*pharmacology MH - Disinfection/instrumentation/*methods MH - Equipment and Supplies, Hospital/*microbiology MH - Hospitals MH - Humans MH - Spores, Bacterial/*drug effects/growth & development OTO - NOTNLM OT - Clostridium difficile OT - Infection control and prevention OT - Spores OT - Washer disinfector EDAT- 2019/08/08 06:00 MHDA- 2020/03/12 06:00 CRDT- 2019/08/08 06:00 PHST- 2019/05/17 00:00 [received] PHST- 2019/07/11 00:00 [revised] PHST- 2019/07/15 00:00 [accepted] PHST- 2019/08/08 06:00 [pubmed] PHST- 2020/03/12 06:00 [medline] PHST- 2019/08/08 06:00 [entrez] AID - S2468-0451(19)30037-9 [pii] AID - 10.1016/j.idh.2019.07.001 [doi] PST - ppublish SO - Infect Dis Health. 2019 Nov;24(4):208-211. doi: 10.1016/j.idh.2019.07.001. Epub 2019 Aug 3.