PMID- 31389573
OWN - NLM
STAT- MEDLINE
DCOM- 20200904
LR  - 20200904
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 3 Suppl
DP  - 2019 Aug
TI  - Repair of spinal cord injury in rats by umbilical cord mesenchymal stem cells 
      through P38MAPK signaling pathway.
PG  - 47-53
LID - 18627 [pii]
LID - 10.26355/eurrev_201908_18627 [doi]
AB  - OBJECTIVE: To explore the repair of spinal cord injury (SCI) in rats by umbilical 
      cord mesenchymal stem cells (UCMSCs) through the p38mitogen-activated protein 
      kinase (MAPK) signaling pathway. MATERIALS AND METHODS: A total of 45 healthy 
      adult male Sprague-Dawley rats weighing 180-220 g and aged 6-8 weeks old were 
      randomly divided into group A (SCI model + transplantation of UCMSCs, n=15), 
      group B (sham operation), and group C (SCI model + injection of an equal dose of 
      DMEM, n=15) using a random number table. The morphology of spinal cord tissues 
      was observed via hematoxylin-eosin (HE) staining, and the protein expression of 
      phosphorylated p38 (p-p38) in spinal cord tissues, the expression of glial 
      fibrillary acidic protein (GFAP) in the injury region, and the spinal cord 
      neuronal apoptosis were detected via Western blotting, immunofluorescence 
      labeling and terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) assay, respectively. RESULTS: In group B, there was no 
      significant damage to the structure of spinal cord tissues. In group C, the 
      spinal cord tissues had a disordered structure and significant fragmentation, the 
      damage to grey matter was the greatest. Also, almost all of the grey matter was 
      destroyed and dissolved, with a large number of scars and cavitation, and it was 
      hard to distinguish the gray matter and white matter. In group A, the spinal cord 
      tissues had a clear structure, there were smaller necrotic cavitation regions in 
      the grey-white matter, and the number of cavitation significantly declined 
      compared with that in group C. The results of immunofluorescence assay revealed 
      that the expression of GFAP in spinal cord tissues was the lowest in group B, 
      while it was remarkably decreased in group A compared with that in group C 
      (p<0.05), suggesting that injecting UCMSCs via the caudal vein can prominently 
      reduce the expression of GFAP in spinal cord tissues. Moreover, the spinal cord 
      neuronal apoptosis rate was (4.21+/-0.19), (0.72+/-0.21) and (4.57+/-0.31), 
      respectively, in group A, group B, and group C. It can be seen that the spinal 
      cord neuronal apoptosis rate significantly declined in group A due to the 
      treatment with UCMSCs. Also, the significant difference compared with that in 
      group C, while it was significantly increased in group A compared with that in 
      group B, but lower than group C (p<0.05). According to the results of Western 
      blotting, the protein expression of p-p38 in spinal cord tissues was remarkably 
      decreased in group B compared with that in group A and group C (p<0.05), while it 
      was also markedly decreased in group A compared with that in group C (p<0.05), 
      indicating that injecting UCMSCs via the caudal vein can significantly lower the 
      protein expression of p-p38 in spinal cord tissues. CONCLUSIONS: UCMSCs promote 
      the recovery of neurological function, inhibit the p38 MAPK pathway activated 
      after SCI, and reduce the spinal cord neuronal apoptosis in SCI rats.
FAU - Tian, D-Z
AU  - Tian DZ
AD  - Department of Neurosurgery, Cardiovascular Specialist Units, Affiliated Hospital 
      of Yanan University, Yan'an, Shaanxi, China. zhuq968@163.com.
FAU - Deng, D
AU  - Deng D
FAU - Qiang, J-L
AU  - Qiang JL
FAU - Zhu, Q
AU  - Zhu Q
FAU - Li, Q-C
AU  - Li QC
FAU - Yi, Z-G
AU  - Yi ZG
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (GFAP protein, rat)
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Glial Fibrillary Acidic Protein/*metabolism
MH  - MAP Kinase Signaling System
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/cytology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/metabolism/*therapy
MH  - Umbilical Cord/*cytology
EDAT- 2019/08/08 06:00
MHDA- 2020/09/05 06:00
CRDT- 2019/08/08 06:00
PHST- 2019/08/08 06:00 [entrez]
PHST- 2019/08/08 06:00 [pubmed]
PHST- 2020/09/05 06:00 [medline]
AID - 18627 [pii]
AID - 10.26355/eurrev_201908_18627 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Aug;23(3 Suppl):47-53. doi: 
      10.26355/eurrev_201908_18627.