PMID- 31389590
OWN - NLM
STAT- MEDLINE
DCOM- 20200918
LR  - 20200918
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 3 Suppl
DP  - 2019 Aug
TI  - Glutamine has a protective role on intestinal tissues via targeting NF-kappaB pathway 
      in rats with sepsis.
PG  - 184-191
LID - 18646 [pii]
LID - 10.26355/eurrev_201908_18646 [doi]
AB  - OBJECTIVE: To study the protective effect of glutamine on the intestinal tissues 
      of septic rats by regulating the nuclear factor-kappaB (NF-kappaB) pathway. MATERIALS AND 
      METHODS: A total of 30 rats were divided into the Sham group, Model group, and 
      Glutamine group using a random number table. The changes in the intestinal 
      tissues in rats were observed via hematoxylin-eosin (HE) staining, and the 
      difference in the content of serum inflammatory factor tumor necrosis factor-alpha 
      (TNF-alpha) was detected via enzyme-linked immunosorbent assay (ELISA). Moreover, the 
      apoptosis of the intestinal tissues was detected via terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay, and the protein 
      expression of NF-kappaB in intestinal tissues was detected via Western blotting. 
      RESULTS: In the Sham group, the rats had normal activity and good mental state, 
      and there were no evident lesions in the abdominal cavity. Compared with the rats 
      in the Sham group, the rats in the Model group had very poor mental state and 
      erected hair, and they trembled and barely moved. After the abdomen was opened, 
      there were bad smell and evident bleeding in the abdominal cavity, and the cecum 
      became black with adhesion and swelling. In the Glutamine group, the symptoms 
      were significantly alleviated compared with the Model group. The morphological 
      observation of the intestinal tissues revealed that in the Sham group, the 
      intestinal villi were regularly and clearly arranged, and there was no congestion 
      in the capillaries. Compared with the Sham group, the intestinal villi were 
      disorderly arranged with rupture in the Model group, and the severe capillary 
      congestion was clearly visible and accompanied by ulcer. In the Glutamine group, 
      the intestinal villi had normal morphology and regular arrangement after 
      treatment, the subepithelial space was significantly dilated, the capillary 
      dilation and the congestion could be seen and the lamina propria was intact. In 
      the Sham group, the pathological score was 0 point, and the intestinal mucosa and 
      villi had normal structure. Compared with that in the Sham group, the 
      pathological score of the intestinal tissues were significantly increased in the 
      Model group (p<0.05). In the Glutamine group, the pathological score 
      significantly declined after treatment compared with that in the Model group 
      (p<0.05). Besides, the content of the inflammatory factor TNF-alpha in the intestinal 
      tissues was the highest in the Model group (p<0.05), and it was lower in the 
      Glutamine group than that in the Sham group (p<0.05), indicating that glutamine 
      can effectively reduce the content of the inflammatory factor TNF-alpha, exerting a 
      certain protective effect on the intestinal tissues. The number of apoptotic 
      intestinal epithelial cells was remarkably increased in the Model group compared 
      with that in the Sham group (p<0.05), and it was remarkably decreased in the 
      Glutamine group compared with that in the Model group (p<0.05). The Model group 
      had a significantly higher protein expression of NF-kappaB in intestinal tissues than 
      in the Sham group and Glutamine group (p<0.05), Sham group had the lowest protein 
      expression of NF-kappaB in intestinal tissues (p<0.05), and the Glutamine group had a 
      significantly lower protein expression of NF-kappaB in intestinal tissues than the 
      Model group (p<0.05). CONCLUSIONS: Glutamine inhibits the protein expression of 
      NF-kappaB, thereby exerting a protective effect on intestinal tissues of sepsis rats.
FAU - Wu, T-Y
AU  - Wu TY
AD  - Department of Critical Care Medicine, Haijiya Hospital, Shanxian County, Heze, 
      China. 155233475@qq.com.
FAU - Zhang, H-B
AU  - Zhang HB
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (NF-kappa B)
RN  - 0RH81L854J (Glutamine)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Glutamine/*administration & dosage/pharmacology
MH  - Intestinal Mucosa/*cytology/drug effects/metabolism
MH  - NF-kappa B/*metabolism
MH  - Rats
MH  - Sepsis/*drug therapy/metabolism
MH  - Signal Transduction/drug effects
EDAT- 2019/08/08 06:00
MHDA- 2020/09/20 06:00
CRDT- 2019/08/08 06:00
PHST- 2019/08/08 06:00 [entrez]
PHST- 2019/08/08 06:00 [pubmed]
PHST- 2020/09/20 06:00 [medline]
AID - 18646 [pii]
AID - 10.26355/eurrev_201908_18646 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Aug;23(3 Suppl):184-191. doi: 
      10.26355/eurrev_201908_18646.