PMID- 31389596
OWN - NLM
STAT- MEDLINE
DCOM- 20200918
LR  - 20200918
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 3 Suppl
DP  - 2019 Aug
TI  - LncRNA MEG3 accelerates apoptosis of hypoxic myocardial cells via FoxO1 signaling 
      pathway.
PG  - 334-340
LID - 18665 [pii]
LID - 10.26355/eurrev_201908_18665 [doi]
AB  - OBJECTIVE: To explore the effects of long non-coding ribonucleic acid (lncRNA) 
      maternally expressed gene 3 (MEG3) on the proliferation and apoptosis of hypoxic 
      myocardial cells by regulating the expression of forkhead box O1 (FoxO1). 
      MATERIALS AND METHODS: The myocardial A10 cell lines were divided into myocardial 
      cell group (group A), hypoxic myocardial cell group (group B), and hypoxic 
      myocardial cell + transfection with lncRNA MEG3 mimic group (group C). The 
      correlations of the adenosine triphosphate (ATP) concentration, the degree of 
      apoptosis, and the proliferation with FoxO1 and FoxO3a proteins in the cells were 
      observed via ATP assay, Cell Counting Kit-8 (CCK-8) assay, terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, 
      Western blotting, and quantitative Reverse Transcription-Polymerase Chain 
      Reaction (qRT-PCR), respectively. RESULTS: The ATP concentration in myocardial 
      cells was the highest in group A (p<0.05), and it was higher in group B than that 
      in group C (p<0.05). The results of the CCK-8 assay showed that the proliferation 
      rate of the myocardial cells was the highest in group A and the lowest in group C 
      (p<0.05), and it was significantly increased in group B compared with that in 
      group C (p<0.05). The results of the TUNEL assay revealed that the normal cells 
      displayed the purple color, and apoptotic cells displayed the green color. The 
      myocardial cells were arranged orderly, and the number of apoptotic cells was 
      smaller in group A, the number of apoptotic cells was significantly larger in 
      group B than that in group A, and it was the largest in group C (p<0.05). 
      Moreover, the results of Western blotting manifested that the concentrations of 
      FoxO1 and FoxO3 proteins in myocardial cells were the lowest in group A (p<0.05), 
      and they were significantly higher in group C than those in group B (p<0.05). 
      According to the results of qRT-PCR, the mRNA expressions of FoxO1 and FoxO3 in 
      myocardial cells were the lowest in group A (p<0.05), and they were remarkably 
      lower in group B than those in group C (p<0.05). CONCLUSIONS: LncRNA MEG3 can 
      increase the activity of FoxO1 to promote myocardial apoptosis in a hypoxic 
      environment.
FAU - Zhao, L-Y
AU  - Zhao LY
AD  - Medical Treatment Center, Weifang People's Hospital Brain Hospital, Weifang, 
      China. Xuying169@163.com.
FAU - Li, X
AU  - Li X
FAU - Gao, L
AU  - Gao L
FAU - Xu, Y
AU  - Xu Y
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (FOXO1 protein, human)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (MEG3 non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Apoptosis
MH  - Cell Hypoxia
MH  - Cell Line
MH  - Cell Proliferation
MH  - Forkhead Box Protein O1/*genetics/*metabolism
MH  - Humans
MH  - Myocytes, Cardiac/*cytology/metabolism
MH  - RNA, Long Noncoding/*genetics
MH  - Signal Transduction
EDAT- 2019/08/08 06:00
MHDA- 2020/09/20 06:00
CRDT- 2019/08/08 06:00
PHST- 2019/08/08 06:00 [entrez]
PHST- 2019/08/08 06:00 [pubmed]
PHST- 2020/09/20 06:00 [medline]
AID - 18665 [pii]
AID - 10.26355/eurrev_201908_18665 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Aug;23(3 Suppl):334-340. doi: 
      10.26355/eurrev_201908_18665.