PMID- 313901
OWN - NLM
STAT- MEDLINE
DCOM- 19791024
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 37
IP  - 2
DP  - 1979 Jun
TI  - Generation of memory cells. III. Antibody class requirements for the generation 
      of B-memory cells by antigen--antibody complexes.
PG  - 345-51
AB  - Immunizing mice with preformed antigen--antibody complexes is a highly effective 
      means of generating B-memory cells. In the present study we have compared the 
      capacity of mouse anti-dinitrophenyl (DNP) IgM, IgG1, IgG2 and IgA antibodies to 
      generate DNP-specific memory, when given in complex with antigen (DNP-KLH: 
      keyhole limpet haemocyanin). Two monoclonal IgM antibodies exerted no adjuvant 
      effect, whereas a monoclonal IgA antibody was effective, IgG2 antibodies were a 
      more powerful adjuvant than IgG1, regardless of whether anti-DNP or anti-KLH 
      antibodies were used. Furthermore, the capacity of DNP-KLH--antibody complexes to 
      localize in splenic lymphoid follicles could be ranked IgG2 greater than IgG1 
      greater than IgA; IgM complexes did not localize in follicles. These results 
      correlate well with data (presented elsewhere) on the capacity of these different 
      antibodies to activate mouse complement, and confirm that C activation is an 
      essential requirement for both follicular localization of immune complexes, and 
      for the generation of B-memory cells. Although activation of the alternative 
      complement pathway is sufficient to effect both processes, the results with IgG2 
      antibodies raise the possibility that classical pathway activation may be more 
      effective.
FAU - Klaus, G G
AU  - Klaus GG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - Animals
MH  - *Antigen-Antibody Complex
MH  - B-Lymphocytes/*immunology
MH  - Dinitrobenzenes/immunology
MH  - Immunoglobulin A/immunology
MH  - Immunoglobulin G/immunology
MH  - Immunoglobulin M/immunology
MH  - Immunoglobulins/*immunology
MH  - *Immunologic Memory
MH  - Male
MH  - Mice
MH  - Spleen/immunology
PMC - PMC1457494
EDAT- 1979/06/01 00:00
MHDA- 1979/06/01 00:01
PMCR- 1980/06/01
CRDT- 1979/06/01 00:00
PHST- 1979/06/01 00:00 [pubmed]
PHST- 1979/06/01 00:01 [medline]
PHST- 1979/06/01 00:00 [entrez]
PHST- 1980/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1979 Jun;37(2):345-51.