PMID- 31391353
OWN - NLM
STAT- MEDLINE
DCOM- 20200422
LR  - 20200422
IS  - 1348-4540 (Electronic)
IS  - 0918-8959 (Linking)
VI  - 66
IP  - 11
DP  - 2019 Nov 28
TI  - The injury of recurrent laryngeal nerve and thyroid regeneration after 
      irreversible electroporation ablation of most part of thyroid gland-an 
      experimental study on swine model.
PG  - 1017-1027
LID - 10.1507/endocrj.EJ19-0095 [doi]
AB  - To study the thyroid regeneration and injury of recurrent laryngeal nerve after 
      irreversible electroporation (IRE). 12 pigs were divided into three groups: six 
      pigs underwent IRE, other pigs were used as controls. IRE was performed near 
      tracheoesophageal groove, to ablate most part of thyroid gland. Parathyroid and 
      thyroid function, recurrent laryngeal nerve injury and thyroid computed 
      tomography (CT) imaging were regularly investigated. The histopathology results 
      were analyzed to detect thyroid regeneration. Masson's trichrome method for 
      collagen and immunohistochemistry were performed for Soluble protein-100 (S100) 
      and neurofilaments on nerve section. In IRE group, there were no symptoms of 
      recurrent laryngeal nerve-related injury. No abnormalities of recurrent laryngeal 
      nerve were shown on hematoxylin-eosin (HE) staining, Masson's trichrome staining, 
      Neurofilament (NF) staining and S100 staining. There were no significant changes 
      for thyroid and parathyroid function in all pigs. Immediately after IRE, CT 
      showed hypoattenuation in the ablated thyroid gland and it became swelling. 14 
      days after IRE, thyroid CT showed hetergenous attenuation in the electroporation 
      zone, and the size and attenuation of thyroid gland were normal after two months. 
      There was cell apoptosis in the thyroid gland after IRE. Seven and 14 days after 
      IRE, there was fragmentation of nucleus within the follicle, and some follicles 
      were empty. Two months later, complete regeneration of thyroid tissue was shown. 
      IRE was shown to be both effective and safe with complete regeneration of thyroid 
      tissue and preservation of the function and structure of the recurrent laryngeal 
      nerve.
FAU - Li, Sheng
AU  - Li S
AD  - Department of Radiology, Sun Yat-sen University Cancer Center, State Key 
      Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer 
      Medicine, Guangzhou, Guangdong 510060, P. R. China.
FAU - Yun, Miao
AU  - Yun M
AD  - Department of Ultrasound, Sun Yat-sen University Cancer Center, State Key 
      Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer 
      Medicine, Guangzhou, Guangdong 510060, P. R. China.
FAU - Tian, Li
AU  - Tian L
AD  - Department of Radiology, Sun Yat-sen University Cancer Center, State Key 
      Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer 
      Medicine, Guangzhou, Guangdong 510060, P. R. China.
FAU - Wu, Peihong
AU  - Wu P
AD  - Department of Radiology, Sun Yat-sen University Cancer Center, State Key 
      Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer 
      Medicine, Guangzhou, Guangdong 510060, P. R. China.
FAU - Liu, Longzhong
AU  - Liu L
AD  - Department of Ultrasound, Sun Yat-sen University Cancer Center, State Key 
      Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer 
      Medicine, Guangzhou, Guangdong 510060, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20190807
PL  - Japan
TA  - Endocr J
JT  - Endocrine journal
JID - 9313485
RN  - 0 (S100 Proteins)
SB  - IM
MH  - Ablation Techniques/*methods
MH  - Animals
MH  - Apoptosis
MH  - Electroporation/*methods
MH  - Immunohistochemistry
MH  - Intermediate Filaments/metabolism
MH  - Recurrent Laryngeal Nerve/metabolism
MH  - Recurrent Laryngeal Nerve Injuries/*epidemiology/metabolism
MH  - *Regeneration
MH  - S100 Proteins/metabolism
MH  - Swine
MH  - Swine, Miniature
MH  - Thyroid Gland/diagnostic imaging/physiology/*surgery
MH  - Tomography, X-Ray Computed
OTO - NOTNLM
OT  - Computed tomography
OT  - Irreversible electroporation
OT  - Pathology
OT  - Recurrent laryngeal nerve
OT  - Thyroid regeneration
EDAT- 2019/08/09 06:00
MHDA- 2020/04/23 06:00
CRDT- 2019/08/09 06:00
PHST- 2019/08/09 06:00 [pubmed]
PHST- 2020/04/23 06:00 [medline]
PHST- 2019/08/09 06:00 [entrez]
AID - 10.1507/endocrj.EJ19-0095 [doi]
PST - ppublish
SO  - Endocr J. 2019 Nov 28;66(11):1017-1027. doi: 10.1507/endocrj.EJ19-0095. Epub 2019 
      Aug 7.