PMID- 31392095
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 7
DP  - 2019
TI  - Characterization of the karyotype and accumulation of repetitive sequences in 
      Australian Darling hardyhead Craterocephalus amniculus (Atheriniformes, 
      Teleostei).
PG  - e7347
LID - 10.7717/peerj.7347 [doi]
LID - e7347
AB  - Belonging to the order Atheriniformes, Craterocephalus is one of the most 
      widespread genera of freshwater fishes in Australia, spanning along the northern 
      coast from central Western Australia to central New South Wales and across the 
      Murray-Darling and Lake Eyre basins. In this study, both conventional cytogenetic 
      techniques (Giemsa, C-banding, CMA(3)/DAPI staining), and fluorescence in situ 
      hybridization (FISH) with telomeric DNA and rDNA probes were used to examine the 
      karyotypes and other chromosomal characteristics of Darling hardyhead 
      (Craterocephalus amniculus) from New South Wales, Australia. We identified a 
      diploid chromosome number 2n = 48 (NF = 58) in all studied individuals. FISH with 
      rDNA probes showed a nonsyntenic pattern, with signals on one pair of 
      subtelocentric chromosomes for 5S rDNA and one pair of submetacentric chromosomes 
      for 28S rDNA. C-banding displayed the accumulation of constitutive 
      heterochromatin in the centromeric regions of approximately 40 chromosomes. 
      CMA(3)/DAPI fluorescence staining revealed extremely GC-rich signals in the 
      pericentromeric region of one submetacentric chromosomal pair with size 
      polymorphism. We detected telomeric signals at the end of all chromosomes and no 
      interstitial signals.
FAU - Majtanova, Zuzana
AU  - Majtanova Z
AD  - Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech 
      Academy of Sciences, Libechov, Czech Republic.
FAU - Moy, Karl G
AU  - Moy KG
AD  - Institute for Applied Ecology, University of Canberra, Canberra, Australian 
      Capital Territory, Australia.
FAU - Unmack, Peter J
AU  - Unmack PJ
AD  - Institute for Applied Ecology, University of Canberra, Canberra, Australian 
      Capital Territory, Australia.
FAU - Rab, Petr
AU  - Rab P
AD  - Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech 
      Academy of Sciences, Libechov, Czech Republic.
FAU - Ezaz, Tariq
AU  - Ezaz T
AD  - Institute for Applied Ecology, University of Canberra, Canberra, Australian 
      Capital Territory, Australia.
LA  - eng
PT  - Journal Article
DEP - 20190730
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC6677120
OTO - NOTNLM
OT  - Atherinidae
OT  - Chromosomal markers
OT  - Chromosomes
OT  - FISH
OT  - Fish cytotaxonomy
OT  - Heterochromatin
OT  - Karyotype
OT  - Telomeres
OT  - rDNA
COIS- The authors declare there are no competing interests.
EDAT- 2019/08/09 06:00
MHDA- 2019/08/09 06:01
PMCR- 2019/07/30
CRDT- 2019/08/09 06:00
PHST- 2019/04/18 00:00 [received]
PHST- 2019/06/25 00:00 [accepted]
PHST- 2019/08/09 06:00 [entrez]
PHST- 2019/08/09 06:00 [pubmed]
PHST- 2019/08/09 06:01 [medline]
PHST- 2019/07/30 00:00 [pmc-release]
AID - 7347 [pii]
AID - 10.7717/peerj.7347 [doi]
PST - epublish
SO  - PeerJ. 2019 Jul 30;7:e7347. doi: 10.7717/peerj.7347. eCollection 2019.